Alginate-hydroxypropyl cellulose hydrogel microbeads for alkaline phosphatase encapsulation
- Author
- Anna Karewicz, Katarzyna Zasada, Dorota Bielska, Timothy Douglas (UGent) , John Jansen, Sander Leeuwenburgh and Maria Nowakowska
- Organization
- Abstract
- There is a growing interest in using proteins as therapeutics agents. Unfortunately, they suffer from limited stability and bioavailability. We aimed to develop a new delivery system for proteins. ALP, a model protein, was successfully encapsulated in the physically cross-linked sodium alginate/hydroxypropylcellulose (ALG-HPC) hydrogel microparticles. The obtained objects had regular, spherical shape and a diameter of similar to 4 mu m, as confirmed by optical microscopy and SEM analysis. The properties of the obtained microbeads could be controlled by temperature and additional coating or crosslinking procedures. The slow, sustained release of ALP in its active form with no initial burst effect was observed for chitosan-coated microspheres at pH = 7.4 and 37 degrees C. Activity of ALP released from ALG/HPC microspheres was confirmed by the occurance of effectively induced mineralization. SEM and AFM images revealed formation of the interpenetrated three-dimensional network of mineral, originating from the microbeads' surfaces. FTIR and XRD analyses confirmed formation of hydroxyapatite.
- Keywords
- controlled release, Biomineralization, hydrogels, microspheres, polysaccharides, protein delivery, IN-VITRO, MINERALIZATION, CHITOSAN, PROTEINS, COLLAGEN, COLITIS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-3218381
- MLA
- Karewicz, Anna, et al. “Alginate-Hydroxypropyl Cellulose Hydrogel Microbeads for Alkaline Phosphatase Encapsulation.” JOURNAL OF MICROENCAPSULATION, vol. 31, no. 1, 2014, pp. 68–76, doi:10.3109/02652048.2013.805841.
- APA
- Karewicz, A., Zasada, K., Bielska, D., Douglas, T., Jansen, J., Leeuwenburgh, S., & Nowakowska, M. (2014). Alginate-hydroxypropyl cellulose hydrogel microbeads for alkaline phosphatase encapsulation. JOURNAL OF MICROENCAPSULATION, 31(1), 68–76. https://doi.org/10.3109/02652048.2013.805841
- Chicago author-date
- Karewicz, Anna, Katarzyna Zasada, Dorota Bielska, Timothy Douglas, John Jansen, Sander Leeuwenburgh, and Maria Nowakowska. 2014. “Alginate-Hydroxypropyl Cellulose Hydrogel Microbeads for Alkaline Phosphatase Encapsulation.” JOURNAL OF MICROENCAPSULATION 31 (1): 68–76. https://doi.org/10.3109/02652048.2013.805841.
- Chicago author-date (all authors)
- Karewicz, Anna, Katarzyna Zasada, Dorota Bielska, Timothy Douglas, John Jansen, Sander Leeuwenburgh, and Maria Nowakowska. 2014. “Alginate-Hydroxypropyl Cellulose Hydrogel Microbeads for Alkaline Phosphatase Encapsulation.” JOURNAL OF MICROENCAPSULATION 31 (1): 68–76. doi:10.3109/02652048.2013.805841.
- Vancouver
- 1.Karewicz A, Zasada K, Bielska D, Douglas T, Jansen J, Leeuwenburgh S, et al. Alginate-hydroxypropyl cellulose hydrogel microbeads for alkaline phosphatase encapsulation. JOURNAL OF MICROENCAPSULATION. 2014;31(1):68–76.
- IEEE
- [1]A. Karewicz et al., “Alginate-hydroxypropyl cellulose hydrogel microbeads for alkaline phosphatase encapsulation,” JOURNAL OF MICROENCAPSULATION, vol. 31, no. 1, pp. 68–76, 2014.
@article{3218381,
abstract = {{There is a growing interest in using proteins as therapeutics agents. Unfortunately, they suffer from limited stability and bioavailability. We aimed to develop a new delivery system for proteins. ALP, a model protein, was successfully encapsulated in the physically cross-linked sodium alginate/hydroxypropylcellulose (ALG-HPC) hydrogel microparticles. The obtained objects had regular, spherical shape and a diameter of similar to 4 mu m, as confirmed by optical microscopy and SEM analysis. The properties of the obtained microbeads could be controlled by temperature and additional coating or crosslinking procedures. The slow, sustained release of ALP in its active form with no initial burst effect was observed for chitosan-coated microspheres at pH = 7.4 and 37 degrees C. Activity of ALP released from ALG/HPC microspheres was confirmed by the occurance of effectively induced mineralization. SEM and AFM images revealed formation of the interpenetrated three-dimensional network of mineral, originating from the microbeads' surfaces. FTIR and XRD analyses confirmed formation of hydroxyapatite.}},
author = {{Karewicz, Anna and Zasada, Katarzyna and Bielska, Dorota and Douglas, Timothy and Jansen, John and Leeuwenburgh, Sander and Nowakowska, Maria}},
issn = {{0265-2048}},
journal = {{JOURNAL OF MICROENCAPSULATION}},
keywords = {{controlled release,Biomineralization,hydrogels,microspheres,polysaccharides,protein delivery,IN-VITRO,MINERALIZATION,CHITOSAN,PROTEINS,COLLAGEN,COLITIS}},
language = {{eng}},
number = {{1}},
pages = {{68--76}},
title = {{Alginate-hydroxypropyl cellulose hydrogel microbeads for alkaline phosphatase encapsulation}},
url = {{http://doi.org/10.3109/02652048.2013.805841}},
volume = {{31}},
year = {{2014}},
}
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