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Bayesian modelling to estimate the test characteristics of coprology, coproantigen ELISA and a novel real-time PCR for the diagnosis of taeniasis

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Abstract
Objective : To estimate and compare the performances of coprology, copro-Ag ELISA and real-time polymerase chain reaction assay (copro-PCR) for detection of Taenia solium tapeworm carriers. Methods : The three diagnostic tests were applied on 817 stool samples collected in two Zambian communities where taeniasis is endemic. A Bayesian approach was used to allow estimation of the test characteristics. Two (0.2%; 95% Confidence Interval (CI): 00.8), 67 (8.2%; 95% CI: 6.410.3) and 10 (1.2%; 95% CI: 0.52.2) samples were positive using coprology, copro-Ag ELISA and copro-PCR, respectively. Results : Specificities of 99.9%, 92.0% and 99.0% were determined for coprology, copro-Ag ELISA and copro-PCR, respectively. Sensitivities of 52.5%, 84.5% and 82.7% were determined for coprology, copro-Ag ELISA and copro-PCR, respectively. Conclusions : We urge for additional studies exploring possible cross-reactions of the copro-Ag ELISA and for the use of more sensitive tests, such as copro-PCR, for the detection of tapeworm carriers, which is a key factor in controlling the parasite in endemic areas.
Keywords
specificity, POLYMERASE-CHAIN-REACTION, sensitivity, diagnosis, Bayesian methodology, Taenia solium, taeniasis, SOLIUM TAENIASIS, DIFFERENTIAL-DIAGNOSIS, FECAL SAMPLES, CYSTICERCOSIS, ASSAY, INFECTIONS, FRAMEWORK, SAGINATA

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MLA
Praet, Nicolas, Jaco J Verweij, Kabemba E Mwape, et al. “Bayesian Modelling to Estimate the Test Characteristics of Coprology, Coproantigen ELISA and a Novel Real-time PCR for the Diagnosis of Taeniasis.” TROPICAL MEDICINE & INTERNATIONAL HEALTH 18.5 (2013): 608–614. Print.
APA
Praet, Nicolas, Verweij, J. J., Mwape, K. E., Phiri, I. K., Muma, J. B., Zulu, G., van Lieshout, L., et al. (2013). Bayesian modelling to estimate the test characteristics of coprology, coproantigen ELISA and a novel real-time PCR for the diagnosis of taeniasis. TROPICAL MEDICINE & INTERNATIONAL HEALTH, 18(5), 608–614.
Chicago author-date
Praet, Nicolas, Jaco J Verweij, Kabemba E Mwape, Isaac K Phiri, John B Muma, Gideon Zulu, Lisette van Lieshout, et al. 2013. “Bayesian Modelling to Estimate the Test Characteristics of Coprology, Coproantigen ELISA and a Novel Real-time PCR for the Diagnosis of Taeniasis.” Tropical Medicine & International Health 18 (5): 608–614.
Chicago author-date (all authors)
Praet, Nicolas, Jaco J Verweij, Kabemba E Mwape, Isaac K Phiri, John B Muma, Gideon Zulu, Lisette van Lieshout, Richar Rodriguez-Hidalgo, Washington Benitez-Ortiz, Pierre Dorny, and Sarah Gabriël. 2013. “Bayesian Modelling to Estimate the Test Characteristics of Coprology, Coproantigen ELISA and a Novel Real-time PCR for the Diagnosis of Taeniasis.” Tropical Medicine & International Health 18 (5): 608–614.
Vancouver
1.
Praet N, Verweij JJ, Mwape KE, Phiri IK, Muma JB, Zulu G, et al. Bayesian modelling to estimate the test characteristics of coprology, coproantigen ELISA and a novel real-time PCR for the diagnosis of taeniasis. TROPICAL MEDICINE & INTERNATIONAL HEALTH. 2013;18(5):608–14.
IEEE
[1]
N. Praet et al., “Bayesian modelling to estimate the test characteristics of coprology, coproantigen ELISA and a novel real-time PCR for the diagnosis of taeniasis,” TROPICAL MEDICINE & INTERNATIONAL HEALTH, vol. 18, no. 5, pp. 608–614, 2013.
@article{3214149,
  abstract     = {Objective : To estimate and compare the performances of coprology, copro-Ag ELISA and real-time polymerase chain reaction assay (copro-PCR) for detection of Taenia solium tapeworm carriers.
Methods : The three diagnostic tests were applied on 817 stool samples collected in two Zambian communities where taeniasis is endemic. A Bayesian approach was used to allow estimation of the test characteristics. Two (0.2%; 95% Confidence Interval (CI): 00.8), 67 (8.2%; 95% CI: 6.410.3) and 10 (1.2%; 95% CI: 0.52.2) samples were positive using coprology, copro-Ag ELISA and copro-PCR, respectively.
Results : Specificities of 99.9%, 92.0% and 99.0% were determined for coprology, copro-Ag ELISA and copro-PCR, respectively. Sensitivities of 52.5%, 84.5% and 82.7% were determined for coprology, copro-Ag ELISA and copro-PCR, respectively.
Conclusions : We urge for additional studies exploring possible cross-reactions of the copro-Ag ELISA and for the use of more sensitive tests, such as copro-PCR, for the detection of tapeworm carriers, which is a key factor in controlling the parasite in endemic areas.},
  author       = {Praet, Nicolas and Verweij, Jaco J and Mwape, Kabemba E and Phiri, Isaac K and Muma, John B and Zulu, Gideon and van Lieshout, Lisette and Rodriguez-Hidalgo, Richar and Benitez-Ortiz, Washington and Dorny, Pierre and Gabriël, Sarah},
  issn         = {1360-2276},
  journal      = {TROPICAL MEDICINE & INTERNATIONAL HEALTH},
  keywords     = {specificity,POLYMERASE-CHAIN-REACTION,sensitivity,diagnosis,Bayesian methodology,Taenia solium,taeniasis,SOLIUM TAENIASIS,DIFFERENTIAL-DIAGNOSIS,FECAL SAMPLES,CYSTICERCOSIS,ASSAY,INFECTIONS,FRAMEWORK,SAGINATA},
  language     = {eng},
  number       = {5},
  pages        = {608--614},
  title        = {Bayesian modelling to estimate the test characteristics of coprology, coproantigen ELISA and a novel real-time PCR for the diagnosis of taeniasis},
  url          = {http://dx.doi.org/10.1111/tmi.12089},
  volume       = {18},
  year         = {2013},
}

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