Bayesian modelling to estimate the test characteristics of coprology, coproantigen ELISA and a novel real-time PCR for the diagnosis of taeniasis
- Author
- Nicolas Praet, Jaco J Verweij, Kabemba E Mwape, Isaac K Phiri, John B Muma, Gideon Zulu, Lisette van Lieshout, Richar Rodriguez-Hidalgo, Washington Benitez-Ortiz, Pierre Dorny (UGent) and Sarah Gabriël (UGent)
- Organization
- Abstract
- Objective : To estimate and compare the performances of coprology, copro-Ag ELISA and real-time polymerase chain reaction assay (copro-PCR) for detection of Taenia solium tapeworm carriers. Methods : The three diagnostic tests were applied on 817 stool samples collected in two Zambian communities where taeniasis is endemic. A Bayesian approach was used to allow estimation of the test characteristics. Two (0.2%; 95% Confidence Interval (CI): 00.8), 67 (8.2%; 95% CI: 6.410.3) and 10 (1.2%; 95% CI: 0.52.2) samples were positive using coprology, copro-Ag ELISA and copro-PCR, respectively. Results : Specificities of 99.9%, 92.0% and 99.0% were determined for coprology, copro-Ag ELISA and copro-PCR, respectively. Sensitivities of 52.5%, 84.5% and 82.7% were determined for coprology, copro-Ag ELISA and copro-PCR, respectively. Conclusions : We urge for additional studies exploring possible cross-reactions of the copro-Ag ELISA and for the use of more sensitive tests, such as copro-PCR, for the detection of tapeworm carriers, which is a key factor in controlling the parasite in endemic areas.
- Keywords
- specificity, POLYMERASE-CHAIN-REACTION, sensitivity, diagnosis, Bayesian methodology, Taenia solium, taeniasis, SOLIUM TAENIASIS, DIFFERENTIAL-DIAGNOSIS, FECAL SAMPLES, CYSTICERCOSIS, ASSAY, INFECTIONS, FRAMEWORK, SAGINATA
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-3214149
- MLA
- Praet, Nicolas, Jaco J Verweij, Kabemba E Mwape, et al. “Bayesian Modelling to Estimate the Test Characteristics of Coprology, Coproantigen ELISA and a Novel Real-time PCR for the Diagnosis of Taeniasis.” TROPICAL MEDICINE & INTERNATIONAL HEALTH 18.5 (2013): 608–614. Print.
- APA
- Praet, Nicolas, Verweij, J. J., Mwape, K. E., Phiri, I. K., Muma, J. B., Zulu, G., van Lieshout, L., et al. (2013). Bayesian modelling to estimate the test characteristics of coprology, coproantigen ELISA and a novel real-time PCR for the diagnosis of taeniasis. TROPICAL MEDICINE & INTERNATIONAL HEALTH, 18(5), 608–614.
- Chicago author-date
- Praet, Nicolas, Jaco J Verweij, Kabemba E Mwape, Isaac K Phiri, John B Muma, Gideon Zulu, Lisette van Lieshout, et al. 2013. “Bayesian Modelling to Estimate the Test Characteristics of Coprology, Coproantigen ELISA and a Novel Real-time PCR for the Diagnosis of Taeniasis.” Tropical Medicine & International Health 18 (5): 608–614.
- Chicago author-date (all authors)
- Praet, Nicolas, Jaco J Verweij, Kabemba E Mwape, Isaac K Phiri, John B Muma, Gideon Zulu, Lisette van Lieshout, Richar Rodriguez-Hidalgo, Washington Benitez-Ortiz, Pierre Dorny, and Sarah Gabriël. 2013. “Bayesian Modelling to Estimate the Test Characteristics of Coprology, Coproantigen ELISA and a Novel Real-time PCR for the Diagnosis of Taeniasis.” Tropical Medicine & International Health 18 (5): 608–614.
- Vancouver
- 1.Praet N, Verweij JJ, Mwape KE, Phiri IK, Muma JB, Zulu G, et al. Bayesian modelling to estimate the test characteristics of coprology, coproantigen ELISA and a novel real-time PCR for the diagnosis of taeniasis. TROPICAL MEDICINE & INTERNATIONAL HEALTH. 2013;18(5):608–14.
- IEEE
- [1]N. Praet et al., “Bayesian modelling to estimate the test characteristics of coprology, coproantigen ELISA and a novel real-time PCR for the diagnosis of taeniasis,” TROPICAL MEDICINE & INTERNATIONAL HEALTH, vol. 18, no. 5, pp. 608–614, 2013.
@article{3214149,
abstract = {Objective : To estimate and compare the performances of coprology, copro-Ag ELISA and real-time polymerase chain reaction assay (copro-PCR) for detection of Taenia solium tapeworm carriers.
Methods : The three diagnostic tests were applied on 817 stool samples collected in two Zambian communities where taeniasis is endemic. A Bayesian approach was used to allow estimation of the test characteristics. Two (0.2%; 95% Confidence Interval (CI): 00.8), 67 (8.2%; 95% CI: 6.410.3) and 10 (1.2%; 95% CI: 0.52.2) samples were positive using coprology, copro-Ag ELISA and copro-PCR, respectively.
Results : Specificities of 99.9%, 92.0% and 99.0% were determined for coprology, copro-Ag ELISA and copro-PCR, respectively. Sensitivities of 52.5%, 84.5% and 82.7% were determined for coprology, copro-Ag ELISA and copro-PCR, respectively.
Conclusions : We urge for additional studies exploring possible cross-reactions of the copro-Ag ELISA and for the use of more sensitive tests, such as copro-PCR, for the detection of tapeworm carriers, which is a key factor in controlling the parasite in endemic areas.},
author = {Praet, Nicolas and Verweij, Jaco J and Mwape, Kabemba E and Phiri, Isaac K and Muma, John B and Zulu, Gideon and van Lieshout, Lisette and Rodriguez-Hidalgo, Richar and Benitez-Ortiz, Washington and Dorny, Pierre and Gabriël, Sarah},
issn = {1360-2276},
journal = {TROPICAL MEDICINE & INTERNATIONAL HEALTH},
keywords = {specificity,POLYMERASE-CHAIN-REACTION,sensitivity,diagnosis,Bayesian methodology,Taenia solium,taeniasis,SOLIUM TAENIASIS,DIFFERENTIAL-DIAGNOSIS,FECAL SAMPLES,CYSTICERCOSIS,ASSAY,INFECTIONS,FRAMEWORK,SAGINATA},
language = {eng},
number = {5},
pages = {608--614},
title = {Bayesian modelling to estimate the test characteristics of coprology, coproantigen ELISA and a novel real-time PCR for the diagnosis of taeniasis},
url = {http://dx.doi.org/10.1111/tmi.12089},
volume = {18},
year = {2013},
}
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