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Regulatory microRNA network identification in bovine blastocyst development

Karen Goossens, Pieter Mestdagh (UGent) , Steve Lefever (UGent) , Mario Van Poucke (UGent) , Alex Van Zeveren (UGent) , Ann Van Soom (UGent) , Jo Vandesompele (UGent) and Luc Peelman (UGent)
(2013) STEM CELLS AND DEVELOPMENT. 22(13). p.1907-1920
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Abstract
Mammalian blastocyst formation is characterized by two lineage segregations resulting in the formation of the trophectoderm, the hypoblast, and the epiblast cell lineages. Cell fate determination during these early lineage segregations is associated with changes in the expression of specific transcription factors. In addition to the transcription factor-based control, it has become clear that also microRNAs (miRNAs) play an important role in the post-transcriptional regulation of pluripotency and differentiation. To elucidate the role of miRNAs in early lineage segregation, we compared the miRNA expression in early bovine blastocysts with the more advanced stage of hatched blastocysts. Reverse transcription-quantitative PCR-based miRNA expression profiling revealed eight upregulated miRNAs (miR-127, miR-130a, miR-155, miR-196a, miR-203, miR-28, miR-29c, and miR-376a) and four downregulated miRNAs (miR-135a, miR-218, miR-335, and miR-449b) in hatched blastocysts. Through an integrative analysis of matching miRNA and mRNA expression data, candidate miRNA-mRNA interaction pairs were prioritized for validation. Using an in vitro luciferase reporter assay, we confirmed a direct interaction between miR-218 and CDH2, miR-218 and NANOG, and miR-449b and NOTCH1. By interfering with the FGF signaling pathway, we found functional evidence that miR-218, mainly expressed in the inner cell mass, regulates the NANOG expression in the bovine blastocyst in response to FGF signaling. The results of this study expand our knowledge about the miRNA signature of the bovine blastocyst and of the interactions between miRNAs and cell fate regulating transcription factors.
Keywords
RNA, DIFFERENTIATION, ROLES, GENE-EXPRESSION, MOUSE BLASTOCYST, CADHERIN EXPRESSION, PREIMPLANTATION EMBRYOS, REAL-TIME PCR, EMBRYONIC STEM-CELLS, EARLY LINEAGE SEGREGATION

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Chicago
Goossens, Karen, Pieter Mestdagh, Steve Lefever, Mario Van Poucke, Alex Van Zeveren, Ann Van Soom, Jo Vandesompele, and Luc Peelman. 2013. “Regulatory microRNA Network Identification in Bovine Blastocyst Development.” Stem Cells and Development 22 (13): 1907–1920.
APA
Goossens, Karen, Mestdagh, P., Lefever, S., Van Poucke, M., Van Zeveren, A., Van Soom, A., Vandesompele, J., et al. (2013). Regulatory microRNA network identification in bovine blastocyst development. STEM CELLS AND DEVELOPMENT, 22(13), 1907–1920.
Vancouver
1.
Goossens K, Mestdagh P, Lefever S, Van Poucke M, Van Zeveren A, Van Soom A, et al. Regulatory microRNA network identification in bovine blastocyst development. STEM CELLS AND DEVELOPMENT. 2013;22(13):1907–20.
MLA
Goossens, Karen, Pieter Mestdagh, Steve Lefever, et al. “Regulatory microRNA Network Identification in Bovine Blastocyst Development.” STEM CELLS AND DEVELOPMENT 22.13 (2013): 1907–1920. Print.
@article{3213443,
  abstract     = {Mammalian blastocyst formation is characterized by two lineage segregations resulting in the formation of the trophectoderm, the hypoblast, and the epiblast cell lineages. Cell fate determination during these early lineage segregations is associated with changes in the expression of specific transcription factors. In addition to the transcription factor-based control, it has become clear that also microRNAs (miRNAs) play an important role in the post-transcriptional regulation of pluripotency and differentiation. To elucidate the role of miRNAs in early lineage segregation, we compared the miRNA expression in early bovine blastocysts with the more advanced stage of hatched blastocysts. Reverse transcription-quantitative PCR-based miRNA expression profiling revealed eight upregulated miRNAs (miR-127, miR-130a, miR-155, miR-196a, miR-203, miR-28, miR-29c, and miR-376a) and four downregulated miRNAs (miR-135a, miR-218, miR-335, and miR-449b) in hatched blastocysts. Through an integrative analysis of matching miRNA and mRNA expression data, candidate miRNA-mRNA interaction pairs were prioritized for validation. Using an in vitro luciferase reporter assay, we confirmed a direct interaction between miR-218 and CDH2, miR-218 and NANOG, and miR-449b and NOTCH1. By interfering with the FGF signaling pathway, we found functional evidence that miR-218, mainly expressed in the inner cell mass, regulates the NANOG expression in the bovine blastocyst in response to FGF signaling. The results of this study expand our knowledge about the miRNA signature of the bovine blastocyst and of the interactions between miRNAs and cell fate regulating transcription factors.},
  author       = {Goossens, Karen and Mestdagh, Pieter and Lefever, Steve and Van Poucke, Mario and Van Zeveren, Alex and Van Soom, Ann and Vandesompele, Jo and Peelman, Luc},
  issn         = {1547-3287},
  journal      = {STEM CELLS AND DEVELOPMENT},
  keywords     = {RNA,DIFFERENTIATION,ROLES,GENE-EXPRESSION,MOUSE BLASTOCYST,CADHERIN EXPRESSION,PREIMPLANTATION EMBRYOS,REAL-TIME PCR,EMBRYONIC STEM-CELLS,EARLY LINEAGE SEGREGATION},
  language     = {eng},
  number       = {13},
  pages        = {1907--1920},
  title        = {Regulatory microRNA network identification in bovine blastocyst development},
  url          = {http://dx.doi.org/10.1089/scd.2012.0708},
  volume       = {22},
  year         = {2013},
}

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