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Molecular and physiological analysis of growth-limiting drought stress in Brachypodium distachyon leaves

(2013) MOLECULAR PLANT. 6(2). p.311-322
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Organization
Project
Bioinformatics: from nucleotids to networks (N2N)
Project
Biotechnology for a sustainable economy (Bio-Economy)
Abstract
The drought-tolerant grass Brachypodium distachyon is an emerging model species for temperate grasses and cereal crops. To explore the usefulness of this species for drought studies, a reproducible in vivo drought assay was developed. Spontaneous soil drying led to a 45% reduction in leaf size, and this was mostly due to a decrease in cell expansion, whereas cell division remained largely unaffected by drought. To investigate the molecular basis of the observed leaf growth reduction, the third Brachypodium leaf was dissected in three zones, namely proliferation, expansion, and mature zones, and subjected to transcriptome analysis, based on a whole-genome tiling array. This approach allowed us to highlight that transcriptome profiles of different developmental leaf zones respond differently to drought. Several genes and functional processes involved in drought tolerance were identified. The transcriptome data suggest an increased energy availability in the proliferation zones, along with an up-regulation of sterol synthesis that may influence membrane fluidity. This information may be used to improve the tolerance of temperate cereals to drought, which is undoubtedly one of the major environmental challenges faced by agriculture today and in the near future.
Keywords
WATER, GRASS, ARABIDOPSIS, ABIOTIC STRESSES, Brachypodium, drought stress, plant growth, leaf, PROBE LEVEL DATA, AGROBACTERIUM-MEDIATED TRANSFORMATION, PLANT-GROWTH, CELL-DIVISION, TOLERANCE, TREHALOSE

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Citation

Please use this url to cite or link to this publication:

Chicago
Verelst, Wim, Edoardo Bertolini, Stefanie De Bodt, Klaas Vandepoele, Marlies Demeulenaere, Mario Enrico Pé, and Dirk Inzé. 2013. “Molecular and Physiological Analysis of Growth-limiting Drought Stress in Brachypodium Distachyon Leaves.” Molecular Plant 6 (2): 311–322.
APA
Verelst, W., Bertolini, E., De Bodt, S., Vandepoele, K., Demeulenaere, M., Pé, M. E., & Inzé, D. (2013). Molecular and physiological analysis of growth-limiting drought stress in Brachypodium distachyon leaves. MOLECULAR PLANT, 6(2), 311–322.
Vancouver
1.
Verelst W, Bertolini E, De Bodt S, Vandepoele K, Demeulenaere M, Pé ME, et al. Molecular and physiological analysis of growth-limiting drought stress in Brachypodium distachyon leaves. MOLECULAR PLANT. 2013;6(2):311–22.
MLA
Verelst, Wim, Edoardo Bertolini, Stefanie De Bodt, et al. “Molecular and Physiological Analysis of Growth-limiting Drought Stress in Brachypodium Distachyon Leaves.” MOLECULAR PLANT 6.2 (2013): 311–322. Print.
@article{3211426,
  abstract     = {The drought-tolerant grass Brachypodium distachyon is an emerging model species for temperate grasses and cereal crops. To explore the usefulness of this species for drought studies, a reproducible in vivo drought assay was developed. Spontaneous soil drying led to a 45\% reduction in leaf size, and this was mostly due to a decrease in cell expansion, whereas cell division remained largely unaffected by drought. To investigate the molecular basis of the observed leaf growth reduction, the third Brachypodium leaf was dissected in three zones, namely proliferation, expansion, and mature zones, and subjected to transcriptome analysis, based on a whole-genome tiling array. This approach allowed us to highlight that transcriptome profiles of different developmental leaf zones respond differently to drought. Several genes and functional processes involved in drought tolerance were identified. The transcriptome data suggest an increased energy availability in the proliferation zones, along with an up-regulation of sterol synthesis that may influence membrane fluidity. This information may be used to improve the tolerance of temperate cereals to drought, which is undoubtedly one of the major environmental challenges faced by agriculture today and in the near future.},
  author       = {Verelst, Wim and Bertolini, Edoardo and De Bodt, Stefanie and Vandepoele, Klaas and Demeulenaere, Marlies and P{\'e}, Mario Enrico and Inz{\'e}, Dirk},
  issn         = {1674-2052},
  journal      = {MOLECULAR PLANT},
  keyword      = {WATER,GRASS,ARABIDOPSIS,ABIOTIC STRESSES,Brachypodium,drought stress,plant growth,leaf,PROBE LEVEL DATA,AGROBACTERIUM-MEDIATED TRANSFORMATION,PLANT-GROWTH,CELL-DIVISION,TOLERANCE,TREHALOSE},
  language     = {eng},
  number       = {2},
  pages        = {311--322},
  title        = {Molecular and physiological analysis of growth-limiting drought stress in Brachypodium distachyon leaves},
  url          = {http://dx.doi.org/10.1093/mp/sss098},
  volume       = {6},
  year         = {2013},
}

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