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Molecular and physiological analysis of growth-limiting drought stress in Brachypodium distachyon leaves

Wim Verelst UGent, Edoardo Bertolini, Stefanie De Bodt UGent, Klaas Vandepoele UGent, Marlies Demeulenaere, Mario Enrico Pé and Dirk Inzé UGent (2013) MOLECULAR PLANT. 6(2). p.311-322
abstract
The drought-tolerant grass Brachypodium distachyon is an emerging model species for temperate grasses and cereal crops. To explore the usefulness of this species for drought studies, a reproducible in vivo drought assay was developed. Spontaneous soil drying led to a 45% reduction in leaf size, and this was mostly due to a decrease in cell expansion, whereas cell division remained largely unaffected by drought. To investigate the molecular basis of the observed leaf growth reduction, the third Brachypodium leaf was dissected in three zones, namely proliferation, expansion, and mature zones, and subjected to transcriptome analysis, based on a whole-genome tiling array. This approach allowed us to highlight that transcriptome profiles of different developmental leaf zones respond differently to drought. Several genes and functional processes involved in drought tolerance were identified. The transcriptome data suggest an increased energy availability in the proliferation zones, along with an up-regulation of sterol synthesis that may influence membrane fluidity. This information may be used to improve the tolerance of temperate cereals to drought, which is undoubtedly one of the major environmental challenges faced by agriculture today and in the near future.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
WATER, GRASS, ARABIDOPSIS, ABIOTIC STRESSES, Brachypodium, drought stress, plant growth, leaf, PROBE LEVEL DATA, AGROBACTERIUM-MEDIATED TRANSFORMATION, PLANT-GROWTH, CELL-DIVISION, TOLERANCE, TREHALOSE
journal title
MOLECULAR PLANT
Mol. Plant.
volume
6
issue
2
pages
311 - 322
Web of Science type
Article
Web of Science id
000317006000009
JCR category
PLANT SCIENCES
JCR impact factor
6.605 (2013)
JCR rank
8/199 (2013)
JCR quartile
1 (2013)
ISSN
1674-2052
DOI
10.1093/mp/sss098
project
Bioinformatics: from nucleotids to networks (N2N)
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3211426
handle
http://hdl.handle.net/1854/LU-3211426
date created
2013-05-13 14:35:42
date last changed
2016-12-19 15:46:29
@article{3211426,
  abstract     = {The drought-tolerant grass Brachypodium distachyon is an emerging model species for temperate grasses and cereal crops. To explore the usefulness of this species for drought studies, a reproducible in vivo drought assay was developed. Spontaneous soil drying led to a 45\% reduction in leaf size, and this was mostly due to a decrease in cell expansion, whereas cell division remained largely unaffected by drought. To investigate the molecular basis of the observed leaf growth reduction, the third Brachypodium leaf was dissected in three zones, namely proliferation, expansion, and mature zones, and subjected to transcriptome analysis, based on a whole-genome tiling array. This approach allowed us to highlight that transcriptome profiles of different developmental leaf zones respond differently to drought. Several genes and functional processes involved in drought tolerance were identified. The transcriptome data suggest an increased energy availability in the proliferation zones, along with an up-regulation of sterol synthesis that may influence membrane fluidity. This information may be used to improve the tolerance of temperate cereals to drought, which is undoubtedly one of the major environmental challenges faced by agriculture today and in the near future.},
  author       = {Verelst, Wim and Bertolini, Edoardo and De Bodt, Stefanie and Vandepoele, Klaas and Demeulenaere, Marlies and P{\'e}, Mario Enrico and Inz{\'e}, Dirk},
  issn         = {1674-2052},
  journal      = {MOLECULAR PLANT},
  keyword      = {WATER,GRASS,ARABIDOPSIS,ABIOTIC STRESSES,Brachypodium,drought stress,plant growth,leaf,PROBE LEVEL DATA,AGROBACTERIUM-MEDIATED TRANSFORMATION,PLANT-GROWTH,CELL-DIVISION,TOLERANCE,TREHALOSE},
  language     = {eng},
  number       = {2},
  pages        = {311--322},
  title        = {Molecular and physiological analysis of growth-limiting drought stress in Brachypodium distachyon leaves},
  url          = {http://dx.doi.org/10.1093/mp/sss098},
  volume       = {6},
  year         = {2013},
}

Chicago
Verelst, Wim, Edoardo Bertolini, Stefanie De Bodt, Klaas Vandepoele, Marlies Demeulenaere, Mario Enrico Pé, and Dirk Inzé. 2013. “Molecular and Physiological Analysis of Growth-limiting Drought Stress in Brachypodium Distachyon Leaves.” Molecular Plant 6 (2): 311–322.
APA
Verelst, W., Bertolini, E., De Bodt, S., Vandepoele, K., Demeulenaere, M., Pé, M. E., & Inzé, D. (2013). Molecular and physiological analysis of growth-limiting drought stress in Brachypodium distachyon leaves. MOLECULAR PLANT, 6(2), 311–322.
Vancouver
1.
Verelst W, Bertolini E, De Bodt S, Vandepoele K, Demeulenaere M, Pé ME, et al. Molecular and physiological analysis of growth-limiting drought stress in Brachypodium distachyon leaves. MOLECULAR PLANT. 2013;6(2):311–22.
MLA
Verelst, Wim, Edoardo Bertolini, Stefanie De Bodt, et al. “Molecular and Physiological Analysis of Growth-limiting Drought Stress in Brachypodium Distachyon Leaves.” MOLECULAR PLANT 6.2 (2013): 311–322. Print.