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Overexpression of the trehalase gene AtTRE1 leads to increased drought stress tolerance in Arabidopsis and is involved in abscisic acid-induced stomatal closure

Hilde Van Houtte, Lies Vandesteene, Lorena Lopez Galvis UGent, Liesbeth Lemmens, Ewaut Kissel, Sebastien Carpentier, Regina Feil, Nelson Avonce, Tom Beeckman UGent, John E Lunn, et al. (2013) PLANT PHYSIOLOGY. 161(3). p.1158-1171
abstract
Introduction of microbial trehalose biosynthesis enzymes has been reported to enhance abiotic stress resistance in plants but also resulted in undesirable traits. Here, we present an approach for engineering drought stress tolerance by modifying the endogenous trehalase activity in Arabidopsis (Arabidopsis thaliana). AtTRE1 encodes the Arabidopsis trehalase, the only enzyme known in this species to specifically hydrolyze trehalose into glucose. AtTRE1-overexpressing and Attre1 mutant lines were constructed and tested for their performance in drought stress assays. AtTRE1-overexpressing plants had decreased trehalose levels and recovered better after drought stress, whereas Attre1 mutants had elevated trehalose contents and exhibited a drought-susceptible phenotype. Leaf detachment assays showed that Attre1 mutants lose water faster than wild-type plants, whereas AtTRE1-overexpressing plants have a better water-retaining capacity. In vitro studies revealed that abscisic acid-mediated closure of stomata is impaired in Attre1 lines, whereas the AtTRE1 overexpressors are more sensitive toward abscisic acid-dependent stomatal closure. This observation is further supported by the altered leaf temperatures seen in trehalase-modified plantlets during in vivo drought stress studies. Our results show that overexpression of plant trehalase improves drought stress tolerance in Arabidopsis and that trehalase plays a role in the regulation of stomatal closure in the plant drought stress response.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
TRANSCRIPTION FACTOR, TREHALOSE-6-PHOSPHATE SYNTHASE, ROOT-NODULES, ABIOTIC-STRESS, SIGNAL-TRANSDUCTION, REDOX ACTIVATION, VEGETATIVE GROWTH, STARCH SYNTHESIS, TRANSGENIC PLANTS, GUARD-CELLS
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
161
issue
3
pages
1158 - 1171
Web of Science type
Article
Web of Science id
000316984100010
JCR category
PLANT SCIENCES
JCR impact factor
7.394 (2013)
JCR rank
6/199 (2013)
JCR quartile
1 (2013)
ISSN
0032-0889
DOI
10.1104/pp.112.211391
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3211384
handle
http://hdl.handle.net/1854/LU-3211384
date created
2013-05-13 14:30:19
date last changed
2016-12-19 15:46:52
@article{3211384,
  abstract     = {Introduction of microbial trehalose biosynthesis enzymes has been reported to enhance abiotic stress resistance in plants but also resulted in undesirable traits. Here, we present an approach for engineering drought stress tolerance by modifying the endogenous trehalase activity in Arabidopsis (Arabidopsis thaliana). AtTRE1 encodes the Arabidopsis trehalase, the only enzyme known in this species to specifically hydrolyze trehalose into glucose. AtTRE1-overexpressing and Attre1 mutant lines were constructed and tested for their performance in drought stress assays. AtTRE1-overexpressing plants had decreased trehalose levels and recovered better after drought stress, whereas Attre1 mutants had elevated trehalose contents and exhibited a drought-susceptible phenotype. Leaf detachment assays showed that Attre1 mutants lose water faster than wild-type plants, whereas AtTRE1-overexpressing plants have a better water-retaining capacity. In vitro studies revealed that abscisic acid-mediated closure of stomata is impaired in Attre1 lines, whereas the AtTRE1 overexpressors are more sensitive toward abscisic acid-dependent stomatal closure. This observation is further supported by the altered leaf temperatures seen in trehalase-modified plantlets during in vivo drought stress studies. Our results show that overexpression of plant trehalase improves drought stress tolerance in Arabidopsis and that trehalase plays a role in the regulation of stomatal closure in the plant drought stress response.},
  author       = {Van Houtte, Hilde and Vandesteene, Lies and Lopez Galvis, Lorena and Lemmens, Liesbeth and Kissel, Ewaut and Carpentier, Sebastien and Feil, Regina and Avonce, Nelson and Beeckman, Tom and Lunn, John E and Van Dijck, Patrick},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {TRANSCRIPTION FACTOR,TREHALOSE-6-PHOSPHATE SYNTHASE,ROOT-NODULES,ABIOTIC-STRESS,SIGNAL-TRANSDUCTION,REDOX ACTIVATION,VEGETATIVE GROWTH,STARCH SYNTHESIS,TRANSGENIC PLANTS,GUARD-CELLS},
  language     = {eng},
  number       = {3},
  pages        = {1158--1171},
  title        = {Overexpression of the trehalase gene AtTRE1 leads to increased drought stress tolerance in Arabidopsis and is involved in abscisic acid-induced stomatal closure},
  url          = {http://dx.doi.org/10.1104/pp.112.211391},
  volume       = {161},
  year         = {2013},
}

Chicago
Van Houtte, Hilde, Lies Vandesteene, Lorena Lopez Galvis, Liesbeth Lemmens, Ewaut Kissel, Sebastien Carpentier, Regina Feil, et al. 2013. “Overexpression of the Trehalase Gene AtTRE1 Leads to Increased Drought Stress Tolerance in Arabidopsis and Is Involved in Abscisic Acid-induced Stomatal Closure.” Plant Physiology 161 (3): 1158–1171.
APA
Van Houtte, H., Vandesteene, L., Lopez Galvis, L., Lemmens, L., Kissel, E., Carpentier, S., Feil, R., et al. (2013). Overexpression of the trehalase gene AtTRE1 leads to increased drought stress tolerance in Arabidopsis and is involved in abscisic acid-induced stomatal closure. PLANT PHYSIOLOGY, 161(3), 1158–1171.
Vancouver
1.
Van Houtte H, Vandesteene L, Lopez Galvis L, Lemmens L, Kissel E, Carpentier S, et al. Overexpression of the trehalase gene AtTRE1 leads to increased drought stress tolerance in Arabidopsis and is involved in abscisic acid-induced stomatal closure. PLANT PHYSIOLOGY. 2013;161(3):1158–71.
MLA
Van Houtte, Hilde, Lies Vandesteene, Lorena Lopez Galvis, et al. “Overexpression of the Trehalase Gene AtTRE1 Leads to Increased Drought Stress Tolerance in Arabidopsis and Is Involved in Abscisic Acid-induced Stomatal Closure.” PLANT PHYSIOLOGY 161.3 (2013): 1158–1171. Print.