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M2e-displaying virus-like particles with associated RNA promote T helper 1 type adaptive immunity against influenza A

(2013) PLOS ONE. 8(3).
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Abstract
The ectodomain of influenza A matrix protein 2 (M2e) is a candidate for a universal influenza A vaccine. We used recombinant Hepatitis B core antigen to produce virus-like particles presenting M2e (M2e-VLPs). We produced the VLPs with and without entrapped nucleic acids and compared their immunogenicity and protective efficacy. Immunization of BALB/c mice with M2e-VLPs containing nucleic acids induced a stronger, Th1-biased antibody response compared to particles lacking nucleic acids. The former also induced a stronger M2e-specific CD4(+) T cell response, as determined by ELISPOT. Mice vaccinated with alum-adjuvanted M2e-VLPs containing the nucleic acid-binding domain were better protected against influenza A virus challenge than mice vaccinated with similar particles lacking this domain, as deduced from the loss in body weight following challenge with X47 (H3N2) or PR/8 virus. Challenge of mice that had been immunized with M2e-VLPs with or without nucleic acids displayed significantly lower mortality, morbidity and lung virus titers than control-immunized groups. We conclude that nucleic acids present in M2e-VLPs correlate with improved immune protection.
Keywords
HEPATITIS-B-VIRUS, MATRIX PROTEIN-2, CORE PARTICLES, EXTRACELLULAR DOMAIN, PANDEMIC INFLUENZA, ANTIBODY-RESPONSE, M2 PROTEIN, VACCINE, PROTECTION, IMMUNOGENICITY

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Citation

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Chicago
Ibanez, Lorena, Kenny Roose, Marina De Filette, Michael Schotsaert, Jessica De Sloovere, Stefan Roels, Charlotte Pollard, et al. 2013. “M2e-displaying Virus-like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity Against Influenza A.” Plos One 8 (3).
APA
Ibanez, L., Roose, K., De Filette, M., Schotsaert, M., De Sloovere, J., Roels, S., Pollard, C., et al. (2013). M2e-displaying virus-like particles with associated RNA promote T helper 1 type adaptive immunity against influenza A. PLOS ONE, 8(3).
Vancouver
1.
Ibanez L, Roose K, De Filette M, Schotsaert M, De Sloovere J, Roels S, et al. M2e-displaying virus-like particles with associated RNA promote T helper 1 type adaptive immunity against influenza A. PLOS ONE. 2013;8(3).
MLA
Ibanez, Lorena et al. “M2e-displaying Virus-like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity Against Influenza A.” PLOS ONE 8.3 (2013): n. pag. Print.
@article{3205191,
  abstract     = {The ectodomain of influenza A matrix protein 2 (M2e) is a candidate for a universal influenza A vaccine. We used recombinant Hepatitis B core antigen to produce virus-like particles presenting M2e (M2e-VLPs). We produced the VLPs with and without entrapped nucleic acids and compared their immunogenicity and protective efficacy. Immunization of BALB/c mice with M2e-VLPs containing nucleic acids induced a stronger, Th1-biased antibody response compared to particles lacking nucleic acids. The former also induced a stronger M2e-specific CD4(+) T cell response, as determined by ELISPOT. Mice vaccinated with alum-adjuvanted M2e-VLPs containing the nucleic acid-binding domain were better protected against influenza A virus challenge than mice vaccinated with similar particles lacking this domain, as deduced from the loss in body weight following challenge with X47 (H3N2) or PR/8 virus. Challenge of mice that had been immunized with M2e-VLPs with or without nucleic acids displayed significantly lower mortality, morbidity and lung virus titers than control-immunized groups. We conclude that nucleic acids present in M2e-VLPs correlate with improved immune protection.},
  articleno    = {e59081},
  author       = {Ibanez, Lorena and Roose, Kenny and De Filette, Marina and Schotsaert, Michael and De Sloovere, Jessica and Roels, Stefan and Pollard, Charlotte and Schepens, Bert and Grooten, Johan and Fiers, Walter and Saelens, Xavier},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {HEPATITIS-B-VIRUS,MATRIX PROTEIN-2,CORE PARTICLES,EXTRACELLULAR DOMAIN,PANDEMIC INFLUENZA,ANTIBODY-RESPONSE,M2 PROTEIN,VACCINE,PROTECTION,IMMUNOGENICITY},
  language     = {eng},
  number       = {3},
  pages        = {12},
  title        = {M2e-displaying virus-like particles with associated RNA promote T helper 1 type adaptive immunity against influenza A},
  url          = {http://dx.doi.org/10.1371/journal.pone.0059081},
  volume       = {8},
  year         = {2013},
}

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