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Different 5' leader sequences modulate β-glucuronidase accumulation levels in transgenic Nicotiana tabacum plants

(1995) EUPHYTICA. 85(1-3). p.209-216
Author
Organization
Abstract
Three random synthetic leaders and three naturally-occurring leaders, the tobacco mosaic virus (TMV) coat protein, the satellite tobacco necrosis virus (STNV) and the plant chlorophyll a/b-binding protein (Cab22L), were shown to modulate the beta-glucuronidase reporter protein accumulation levels in transient expression experiments. The same chimeric constructs also confer differential distribution patterns of reporter protein accumulation in stably-transformed tobacco calli or regenerated transgenic plants. When the highest expression levels with a given leader are compared, the 31-nucleotide random leader stimulates translation 20- and 100-fold relative to the 9- and 4-nucleotide synthetic leaders respectively. However, this 31-nucleotide random leader is approx. 2 to 3-fold weaker than the 30-nucleotide STNV leader and even 5-fold weaker than both the 79-nucleotide TMV leader and the 66-nucleotide Cab22L leader. These results confirm the findings in transient expression experiments and stress the importance of the 5'-untranslated region for the production of heterologous proteins in transgenic plants.
Keywords
COPY NUMBER, GENE-EXPRESSION, RNA, REPORTER, SYNTHASE PROMOTERS, 5'-LEADER SEQUENCE, TRANSLATION INVITRO, UNTRANSLATED REGION, TOBACCO NECROSIS VIRUS, CAULIFLOWER MOSAIC-VIRUS, VARIATION OF GENE EXPRESSION, 5'-UNTRANSLATED REGION, TRANSLATIONAL CONTROL, SILENCING, BETA-GLUCURONIDASE, PLANT

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Citation

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Chicago
De Loose, Marc, Xavier Danthinne, Erik Van Bockstaele, Marc Van Montagu, and Anna Depicker. 1995. “Different 5’ Leader Sequences Modulate Β-glucuronidase Accumulation Levels in Transgenic Nicotiana Tabacum Plants.” Euphytica 85 (1-3): 209–216.
APA
De Loose, M., Danthinne, X., Van Bockstaele, E., Van Montagu, M., & Depicker, A. (1995). Different 5’ leader sequences modulate β-glucuronidase accumulation levels in transgenic Nicotiana tabacum plants. EUPHYTICA, 85(1-3), 209–216. Presented at the Eucarpia Genetic Manipulation in Plant Breeding Section Meeting.
Vancouver
1.
De Loose M, Danthinne X, Van Bockstaele E, Van Montagu M, Depicker A. Different 5’ leader sequences modulate β-glucuronidase accumulation levels in transgenic Nicotiana tabacum plants. EUPHYTICA. 1995;85(1-3):209–16.
MLA
De Loose, Marc, Xavier Danthinne, Erik Van Bockstaele, et al. “Different 5’ Leader Sequences Modulate Β-glucuronidase Accumulation Levels in Transgenic Nicotiana Tabacum Plants.” EUPHYTICA 85.1-3 (1995): 209–216. Print.
@article{320403,
  abstract     = {Three random synthetic leaders and three naturally-occurring leaders, the tobacco mosaic virus (TMV) coat protein, the satellite tobacco necrosis virus (STNV) and the plant chlorophyll a/b-binding protein (Cab22L), were shown to modulate the beta-glucuronidase reporter protein accumulation levels in transient expression experiments. The same chimeric constructs also confer differential distribution patterns of reporter protein accumulation in stably-transformed tobacco calli or regenerated transgenic plants. When the highest expression levels with a given leader are compared, the 31-nucleotide random leader stimulates translation 20- and 100-fold relative to the 9- and 4-nucleotide synthetic leaders respectively. However, this 31-nucleotide random leader is approx. 2 to 3-fold weaker than the 30-nucleotide STNV leader and even 5-fold weaker than both the 79-nucleotide TMV leader and the 66-nucleotide Cab22L leader. These results confirm the findings in transient expression experiments and stress the importance of the 5'-untranslated region for the production of heterologous proteins in transgenic plants.},
  author       = {De Loose, Marc and Danthinne, Xavier and Van Bockstaele, Erik and Van Montagu, Marc and Depicker, Anna},
  issn         = {0014-2336},
  journal      = {EUPHYTICA},
  keyword      = {COPY NUMBER,GENE-EXPRESSION,RNA,REPORTER,SYNTHASE PROMOTERS,5'-LEADER SEQUENCE,TRANSLATION INVITRO,UNTRANSLATED REGION,TOBACCO NECROSIS VIRUS,CAULIFLOWER MOSAIC-VIRUS,VARIATION OF GENE EXPRESSION,5'-UNTRANSLATED REGION,TRANSLATIONAL CONTROL,SILENCING,BETA-GLUCURONIDASE,PLANT},
  language     = {eng},
  location     = {Cork, Ireland},
  number       = {1-3},
  pages        = {209--216},
  title        = {Different 5' leader sequences modulate \ensuremath{\beta}-glucuronidase accumulation levels in transgenic Nicotiana tabacum plants},
  url          = {http://dx.doi.org/10.1007/BF00023950},
  volume       = {85},
  year         = {1995},
}

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