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Clathrin-mediated constitutive endocytosis of PIN auxin efflux carriers in Arabidopsis

(2007) CURRENT BIOLOGY. 17(6). p.520-527
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Abstract
Endocytosis is an essential process by which eukaryotic cells internalize exogenous material or regulate signaling At the cell,surface [1]. Different endocytic pathways are well established in yeast and animals; prominent among them is clathrin-dependent endocytosis [2, 3]. In plants, endocytosis is poorly defined, and no molecular mechanism for cargo internalization has been demonstrated so far [4, 5], although the internalization of receptor-ligand complexes at the plant plasma membrane has recently been shown [6]. Here we demonstrate by means of a green-to-red photo-convertible fluorescent reporter, EosFP [7], the constitutive endocytosis of PIN auxin efflux carriers [8] and their recycling to the plasma membrane. Using a plant clathrin-specific antibody, we show the presence of clathrin at different stages of coated-vesicle formation at the plasma membrane in Arabidopsis. Genetic interference with clathrin function inhibits PIN internalization and endocytosis in general. Furthermore, pharmacological interference with cargo recruitment into the clathrin pathway blocks internalization of PINs and other plasma-membrane proteins. Our data demonstrate that clathrin-dependent endocytosis is operational in plants and constitutes the predominant pathway for the internalization of numerous plasma-membrane-resid nt proteins including PIN auxin efflux carriers.
Keywords
FLUORESCENT MARKER, TRANSFERRIN RECEPTOR, TYRPHOSTIN A23, PLANT-CELLS, BREFELDIN-A, TRAFFICKING, TRANSPORT, GRADIENTS, PROTEINS, COMPLEX

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Chicago
Dhonukshe, Pankaj, Fernando Aniento, Inhwan Hwang, David G Robinson, Jozef Mravec, York-Dieter Stierhof, and Jiri Friml. 2007. “Clathrin-mediated Constitutive Endocytosis of PIN Auxin Efflux Carriers in Arabidopsis.” Current Biology 17 (6): 520–527.
APA
Dhonukshe, P., Aniento, F., Hwang, I., Robinson, D. G., Mravec, J., Stierhof, Y.-D., & Friml, J. (2007). Clathrin-mediated constitutive endocytosis of PIN auxin efflux carriers in Arabidopsis. CURRENT BIOLOGY, 17(6), 520–527.
Vancouver
1.
Dhonukshe P, Aniento F, Hwang I, Robinson DG, Mravec J, Stierhof Y-D, et al. Clathrin-mediated constitutive endocytosis of PIN auxin efflux carriers in Arabidopsis. CURRENT BIOLOGY. 2007;17(6):520–7.
MLA
Dhonukshe, Pankaj, Fernando Aniento, Inhwan Hwang, et al. “Clathrin-mediated Constitutive Endocytosis of PIN Auxin Efflux Carriers in Arabidopsis.” CURRENT BIOLOGY 17.6 (2007): 520–527. Print.
@article{3202852,
  abstract     = {Endocytosis is an essential process by which eukaryotic cells internalize exogenous material or regulate signaling At the cell,surface [1]. Different endocytic pathways are well established in yeast and animals; prominent among them is clathrin-dependent endocytosis [2, 3]. In plants, endocytosis is poorly defined, and no molecular mechanism for cargo internalization has been demonstrated so far [4, 5], although the internalization of receptor-ligand complexes at the plant plasma membrane has recently been shown [6]. Here we demonstrate by means of a green-to-red photo-convertible fluorescent reporter, EosFP [7], the constitutive endocytosis of PIN auxin efflux carriers [8] and their recycling to the plasma membrane. Using a plant clathrin-specific antibody, we show the presence of clathrin at different stages of coated-vesicle formation at the plasma membrane in Arabidopsis. Genetic interference with clathrin function inhibits PIN internalization and endocytosis in general. Furthermore, pharmacological interference with cargo recruitment into the clathrin pathway blocks internalization of PINs and other plasma-membrane proteins. Our data demonstrate that clathrin-dependent endocytosis is operational in plants and constitutes the predominant pathway for the internalization of numerous plasma-membrane-resid nt proteins including PIN auxin efflux carriers.},
  author       = {Dhonukshe, Pankaj and Aniento, Fernando and Hwang, Inhwan and Robinson, David G and Mravec, Jozef and Stierhof, York-Dieter and Friml, Jiri},
  issn         = {0960-9822},
  journal      = {CURRENT BIOLOGY},
  language     = {eng},
  number       = {6},
  pages        = {520--527},
  title        = {Clathrin-mediated constitutive endocytosis of PIN auxin efflux carriers in Arabidopsis},
  url          = {http://dx.doi.org/10.1016/j.cub.2007.01.052},
  volume       = {17},
  year         = {2007},
}

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