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Alteration of microtubule dynamic instability during preprophase band formation revealed by yellow fluorescent protein-CLIP170 microtubule plus-end labeling

(2003) PLANT CELL. 15(3). p.597-611
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Abstract
At the onset of mitosis, plant cells form a microtubular preprophase band that defines the plane of cell division, but the mechanism of its formation remains a mystery. Here, we describe the use of mammalian yellow fluorescent protein-tagged CLIP170 to visualize the dynamic plus ends of plant microtubules in transfected cowpea protoplasts and in stably transformed and dividing tobacco Bright Yellow 2 cells. Using plus-end labeling, we observed dynamic instability in different microtubular conformations in live plant cells. The interphase plant microtubules grow at 5 mum/min, shrink at 20 mum/min, and display catastrophe and rescue frequencies of 0.02 and 0.08 events/s, respectively, exhibiting faster turnover than their mammalian counterparts, Strikingly, during preprophase band formation, the growth rate and catastrophe frequency of plant microtubules double, whereas the shrinkage rate and rescue frequency remain unchanged, making microtubules shorter and more dynamic. Using these novel insights and four-dimensional time-lapse imaging data, we propose a model that can explain the mechanism by which changes in microtubule dynamic instability drive the dramatic rearrangements of microtubules during preprophase band and spindle formation in plant cells.
Keywords
ARABIDOPSIS-THALIANA, IN-VIVO, LIVING CELLS, ROOT-TIP CELLS, XENOPUS EGG EXTRACTS, TOBACCO BY-2 CELLS, CYCLE-DEPENDENT CHANGES, CORTICAL MICROTUBULES, HIGHER-PLANTS, CYTOSKELETON

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Chicago
Dhonukshe, Pankaj, and Theodorus WJ Gadella Jr. 2003. “Alteration of Microtubule Dynamic Instability During Preprophase Band Formation Revealed by Yellow Fluorescent protein-CLIP170 Microtubule Plus-end Labeling.” Plant Cell 15 (3): 597–611.
APA
Dhonukshe, P., & Gadella Jr, T. W. (2003). Alteration of microtubule dynamic instability during preprophase band formation revealed by yellow fluorescent protein-CLIP170 microtubule plus-end labeling. PLANT CELL, 15(3), 597–611.
Vancouver
1.
Dhonukshe P, Gadella Jr TW. Alteration of microtubule dynamic instability during preprophase band formation revealed by yellow fluorescent protein-CLIP170 microtubule plus-end labeling. PLANT CELL. 2003;15(3):597–611.
MLA
Dhonukshe, Pankaj, and Theodorus WJ Gadella Jr. “Alteration of Microtubule Dynamic Instability During Preprophase Band Formation Revealed by Yellow Fluorescent protein-CLIP170 Microtubule Plus-end Labeling.” PLANT CELL 15.3 (2003): 597–611. Print.
@article{3202782,
  abstract     = {At the onset of mitosis, plant cells form a microtubular preprophase band that defines the plane of cell division, but the mechanism of its formation remains a mystery. Here, we describe the use of mammalian yellow fluorescent protein-tagged CLIP170 to visualize the dynamic plus ends of plant microtubules in transfected cowpea protoplasts and in stably transformed and dividing tobacco Bright Yellow 2 cells. Using plus-end labeling, we observed dynamic instability in different microtubular conformations in live plant cells. The interphase plant microtubules grow at 5 mum/min, shrink at 20 mum/min, and display catastrophe and rescue frequencies of 0.02 and 0.08 events/s, respectively, exhibiting faster turnover than their mammalian counterparts, Strikingly, during preprophase band formation, the growth rate and catastrophe frequency of plant microtubules double, whereas the shrinkage rate and rescue frequency remain unchanged, making microtubules shorter and more dynamic. Using these novel insights and four-dimensional time-lapse imaging data, we propose a model that can explain the mechanism by which changes in microtubule dynamic instability drive the dramatic rearrangements of microtubules during preprophase band and spindle formation in plant cells.},
  author       = {Dhonukshe, Pankaj and Gadella Jr, Theodorus WJ},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  language     = {eng},
  number       = {3},
  pages        = {597--611},
  title        = {Alteration of microtubule dynamic instability during preprophase band formation revealed by yellow fluorescent protein-CLIP170 microtubule plus-end labeling},
  url          = {http://dx.doi.org/10.1105/tpc.008961},
  volume       = {15},
  year         = {2003},
}

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