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Phospholipase D activation correlates with microtubule reorganization in living plant cells

(2003) PLANT CELL. 15(11). p.2666-2679
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Abstract
A phospholipase D (PLD) was shown recently to decorate microtubules in plant cells. Therefore, we used tobacco BY-2 cells expressing the microtubule reporter GFP-MAP4 to test whether PLD activation affects the organization of plant microtubules. Within 30 min of adding n-butanol, a potent activator of PLD, cortical microtubules were released from the plasma membrane and partially depolymerized, as visualized with four-dimensional confocal imaging. The isomers sec- and tert-butanol, which did not activate PLD, did not affect microtubule organization. The effect of treatment on PLD activation was monitored by the in vivo formation of phosphatidylbutanol, a specific reporter of PLD activity. Tobacco cells also were treated with mastoparan, xylanase, NaCl, and hypoosmotic stress as reported activators of PLD. We confirmed the reports and found that all treatments induced microtubule reorganization and PLD activation within the same time frame. PLD still was activated in microtubule-stabilized (taxol) and microtubule-depolymerized (oryzalin) situations, suggesting that PLD activation triggers microtubular reorganization and not vice versa. Exogenously applied water-soluble synthetic phosphatidic acid did not affect the microtubular cytoskeleton. Cell cycle studies revealed that n-butanol influenced not just interphase cortical microtubules but also those in the preprophase band and phragmoplast, but not those in the spindle structure. Cell growth and division were inhibited in the presence of n-butanol, whereas sec- and tert-butanol had no such effects. Using these novel insights, we propose a model for the mechanism by which PLD activation triggers microtubule reorganization in plant cells.
Keywords
PLASMA-MEMBRANE ROSETTES, DIACYLGLYCEROL PYROPHOSPHATE, ARABIDOPSIS SUSPENSION CELLS, CELLULOSE MICROFIBRIL DEPOSITION, TOBACCO BY-2 CELLS, ABSCISIC-ACID, D-ALPHA, PHOSPHATIDIC-ACID, ACID SIGNAL-TRANSDUCTION, CORTICAL MICROTUBULES

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Citation

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Chicago
Dhonukshe, Pankaj, Ana M Laxalt, Joachim Goedhart, Theodorus WJ Gadella, and Teun Munnik. 2003. “Phospholipase D Activation Correlates with Microtubule Reorganization in Living Plant Cells.” Plant Cell 15 (11): 2666–2679.
APA
Dhonukshe, P., Laxalt, A. M., Goedhart, J., Gadella, T. W., & Munnik, T. (2003). Phospholipase D activation correlates with microtubule reorganization in living plant cells. PLANT CELL, 15(11), 2666–2679.
Vancouver
1.
Dhonukshe P, Laxalt AM, Goedhart J, Gadella TW, Munnik T. Phospholipase D activation correlates with microtubule reorganization in living plant cells. PLANT CELL. 2003;15(11):2666–79.
MLA
Dhonukshe, Pankaj et al. “Phospholipase D Activation Correlates with Microtubule Reorganization in Living Plant Cells.” PLANT CELL 15.11 (2003): 2666–2679. Print.
@article{3202775,
  abstract     = {A phospholipase D (PLD) was shown recently to decorate microtubules in plant cells. Therefore, we used tobacco BY-2 cells expressing the microtubule reporter GFP-MAP4 to test whether PLD activation affects the organization of plant microtubules. Within 30 min of adding n-butanol, a potent activator of PLD, cortical microtubules were released from the plasma membrane and partially depolymerized, as visualized with four-dimensional confocal imaging. The isomers sec- and tert-butanol, which did not activate PLD, did not affect microtubule organization. The effect of treatment on PLD activation was monitored by the in vivo formation of phosphatidylbutanol, a specific reporter of PLD activity. Tobacco cells also were treated with mastoparan, xylanase, NaCl, and hypoosmotic stress as reported activators of PLD. We confirmed the reports and found that all treatments induced microtubule reorganization and PLD activation within the same time frame. PLD still was activated in microtubule-stabilized (taxol) and microtubule-depolymerized (oryzalin) situations, suggesting that PLD activation triggers microtubular reorganization and not vice versa. Exogenously applied water-soluble synthetic phosphatidic acid did not affect the microtubular cytoskeleton. Cell cycle studies revealed that n-butanol influenced not just interphase cortical microtubules but also those in the preprophase band and phragmoplast, but not those in the spindle structure. Cell growth and division were inhibited in the presence of n-butanol, whereas sec- and tert-butanol had no such effects. Using these novel insights, we propose a model for the mechanism by which PLD activation triggers microtubule reorganization in plant cells.},
  author       = {Dhonukshe, Pankaj and Laxalt, Ana M and Goedhart, Joachim and Gadella, Theodorus WJ and Munnik, Teun},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  language     = {eng},
  number       = {11},
  pages        = {2666--2679},
  title        = {Phospholipase D activation correlates with microtubule reorganization in living plant cells},
  url          = {http://dx.doi.org/10.1105/tpc.014977},
  volume       = {15},
  year         = {2003},
}

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