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Microtubule plus-ends reveal essential links between intracellular polarization and localized modulation of endocytosis during division-plane establishment in plant cells

Pankaj Dhonukshe UGent, Jaideep Mathur, Martin Hülskamp and Theodorus WJ Gadella (2005) BMC BIOLOGY. 3.
abstract
Background: A key event in plant morphogenesis is the establishment of a division plane. A plant-specific microtubular preprophase band (PPB) accurately predicts the line of cell division, whereas the phragmoplast, another plant-specific array, executes cell division by maintaining this predicted line. Although establishment of these specific arrays apparently involves intracellular repolarization events that focus cellular resources to a division site, it still remains unclear how microtubules position the cell division planes. Here we study GFP-AtEB1 decorated microtubule plus-ends to dissect events at the division plane. Results: Early mitotic events included guided growth of endoplasmic microtubules (EMTs) towards the PPB site and their coincident localization with endocytic vesicles. Consequently, an endosomal belt lay in close proximity to the microtubular PPB at its maturation and was maintained during spindle formation. During cytokinesis, EMTs radiated from the former spindle poles in a geometrical conformation correlating with cell-plate navigation and tilt-correction. Naphthylphtalamic acid (NPA), an inhibitor of polar auxin efflux, caused abnormal PPBs and shifted division planes. Conclusion: Our observations reveal a spatio-temporal link between microtubules and intracellular polarization essential for localized endocytosis and precise establishment of the division plane in plants. Additionally, they implicate the growth regulator, auxin, in this important cellular event.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PREPROPHASE-BAND FORMATION, TUMOR-SUPPRESSOR PROTEIN, MITOTIC SPINDLE, ENDOPLASMIC-RETICULUM, PLASMA-MEMBRANE, BINDING-PROTEIN, SPATIAL CONTROL, FISSION YEAST, CYTOKINESIS, AUXIN EFFLUX
journal title
BMC BIOLOGY
BMC Biol.
volume
3
article number
11
pages
15 pages
Web of Science type
Article
Web of Science id
000236367100001
ISSN
1741-7007
DOI
10.1186/1741-7007-3-11
language
English
UGent publication?
no
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
3202769
handle
http://hdl.handle.net/1854/LU-3202769
date created
2013-04-26 13:41:05
date last changed
2016-12-21 15:42:06
@article{3202769,
  abstract     = {Background: A key event in plant morphogenesis is the establishment of a division plane. A plant-specific microtubular preprophase band (PPB) accurately predicts the line of cell division, whereas the phragmoplast, another plant-specific array, executes cell division by maintaining this predicted line. Although establishment of these specific arrays apparently involves intracellular repolarization events that focus cellular resources to a division site, it still remains unclear how microtubules position the cell division planes. Here we study GFP-AtEB1 decorated microtubule plus-ends to dissect events at the division plane. 
Results: Early mitotic events included guided growth of endoplasmic microtubules (EMTs) towards the PPB site and their coincident localization with endocytic vesicles. Consequently, an endosomal belt lay in close proximity to the microtubular PPB at its maturation and was maintained during spindle formation. During cytokinesis, EMTs radiated from the former spindle poles in a geometrical conformation correlating with cell-plate navigation and tilt-correction. Naphthylphtalamic acid (NPA), an inhibitor of polar auxin efflux, caused abnormal PPBs and shifted division planes. 
Conclusion: Our observations reveal a spatio-temporal link between microtubules and intracellular polarization essential for localized endocytosis and precise establishment of the division plane in plants. Additionally, they implicate the growth regulator, auxin, in this important cellular event.},
  articleno    = {11},
  author       = {Dhonukshe, Pankaj and Mathur, Jaideep and H{\"u}lskamp, Martin and Gadella, Theodorus WJ},
  issn         = {1741-7007},
  journal      = {BMC BIOLOGY},
  keyword      = {PREPROPHASE-BAND FORMATION,TUMOR-SUPPRESSOR PROTEIN,MITOTIC SPINDLE,ENDOPLASMIC-RETICULUM,PLASMA-MEMBRANE,BINDING-PROTEIN,SPATIAL CONTROL,FISSION YEAST,CYTOKINESIS,AUXIN EFFLUX},
  language     = {eng},
  pages        = {15},
  title        = {Microtubule plus-ends reveal essential links between intracellular polarization and localized modulation of endocytosis during division-plane establishment in plant cells},
  url          = {http://dx.doi.org/10.1186/1741-7007-3-11},
  volume       = {3},
  year         = {2005},
}

Chicago
Dhonukshe, Pankaj, Jaideep Mathur, Martin Hülskamp, and Theodorus WJ Gadella. 2005. “Microtubule Plus-ends Reveal Essential Links Between Intracellular Polarization and Localized Modulation of Endocytosis During Division-plane Establishment in Plant Cells.” Bmc Biology 3.
APA
Dhonukshe, P., Mathur, J., Hülskamp, M., & Gadella, T. W. (2005). Microtubule plus-ends reveal essential links between intracellular polarization and localized modulation of endocytosis during division-plane establishment in plant cells. BMC BIOLOGY, 3.
Vancouver
1.
Dhonukshe P, Mathur J, Hülskamp M, Gadella TW. Microtubule plus-ends reveal essential links between intracellular polarization and localized modulation of endocytosis during division-plane establishment in plant cells. BMC BIOLOGY. 2005;3.
MLA
Dhonukshe, Pankaj, Jaideep Mathur, Martin Hülskamp, et al. “Microtubule Plus-ends Reveal Essential Links Between Intracellular Polarization and Localized Modulation of Endocytosis During Division-plane Establishment in Plant Cells.” BMC BIOLOGY 3 (2005): n. pag. Print.