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PIN proteins perform a rate-limiting function in cellular auxin efflux

(2006) SCIENCE. 312(5775). p.914-918
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Abstract
Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed ( PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport - related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
Keywords
TRANSGENIC PLANTS, ARABIDOPSIS-THALIANA, TRANSPORT, GRADIENTS, GENE, GRAVITROPISM, EXPRESSION, REGULATOR

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MLA
Petrášek, Jan, Jozef Mravec, Rodolphe Bouchard, et al. “PIN Proteins Perform a Rate-limiting Function in Cellular Auxin Efflux.” SCIENCE 312.5775 (2006): 914–918. Print.
APA
Petrášek, J., Mravec, J., Bouchard, R., Blakeslee, J. J., Abas, M., Seifertová, D., Wiśniewska, J., et al. (2006). PIN proteins perform a rate-limiting function in cellular auxin efflux. SCIENCE, 312(5775), 914–918.
Chicago author-date
Petrášek, Jan, Jozef Mravec, Rodolphe Bouchard, Joshua J Blakeslee, Melinda Abas, Daniela Seifertová, Justyna Wiśniewska, et al. 2006. “PIN Proteins Perform a Rate-limiting Function in Cellular Auxin Efflux.” Science 312 (5775): 914–918.
Chicago author-date (all authors)
Petrášek, Jan, Jozef Mravec, Rodolphe Bouchard, Joshua J Blakeslee, Melinda Abas, Daniela Seifertová, Justyna Wiśniewska, Zerihun Tadele, Martin Kubeš, Milada Čovanová, Pankaj Dhonukshe, Petr Skůpa, Eva Benkova, Lucie Perry, Pavel Křeček, Ok Ran Lee, Gerald R Fink, Markus Geisler, Angus S Murphy, Christian Luschnig, Eva Zažímalová, and Jiri Friml. 2006. “PIN Proteins Perform a Rate-limiting Function in Cellular Auxin Efflux.” Science 312 (5775): 914–918.
Vancouver
1.
Petrášek J, Mravec J, Bouchard R, Blakeslee JJ, Abas M, Seifertová D, et al. PIN proteins perform a rate-limiting function in cellular auxin efflux. SCIENCE. 2006;312(5775):914–8.
IEEE
[1]
J. Petrášek et al., “PIN proteins perform a rate-limiting function in cellular auxin efflux,” SCIENCE, vol. 312, no. 5775, pp. 914–918, 2006.
@article{3202731,
  abstract     = {Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed ( PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport - related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.},
  author       = {Petrášek, Jan and Mravec, Jozef and Bouchard, Rodolphe and Blakeslee, Joshua J and Abas, Melinda and Seifertová, Daniela and Wiśniewska, Justyna and Tadele, Zerihun and Kubeš, Martin and Čovanová, Milada and Dhonukshe, Pankaj and Skůpa, Petr and Benkova, Eva and Perry, Lucie and Křeček, Pavel and Lee, Ok Ran and Fink, Gerald R and Geisler, Markus and Murphy, Angus S and Luschnig, Christian and Zažímalová, Eva and Friml, Jiri},
  issn         = {0036-8075},
  journal      = {SCIENCE},
  keywords     = {TRANSGENIC PLANTS,ARABIDOPSIS-THALIANA,TRANSPORT,GRADIENTS,GENE,GRAVITROPISM,EXPRESSION,REGULATOR},
  language     = {eng},
  number       = {5775},
  pages        = {914--918},
  title        = {PIN proteins perform a rate-limiting function in cellular auxin efflux},
  url          = {http://dx.doi.org/10.1126/science.1123542},
  volume       = {312},
  year         = {2006},
}

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