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PIN proteins perform a rate-limiting function in cellular auxin efflux

(2006) SCIENCE. 312(5775). p.914-918
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Abstract
Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed ( PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport - related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
Keywords
TRANSGENIC PLANTS, ARABIDOPSIS-THALIANA, TRANSPORT, GRADIENTS, GENE, GRAVITROPISM, EXPRESSION, REGULATOR

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Chicago
Petrášek, Jan, Jozef Mravec, Rodolphe Bouchard, Joshua J Blakeslee, Melinda Abas, Daniela Seifertová, Justyna Wiśniewska, et al. 2006. “PIN Proteins Perform a Rate-limiting Function in Cellular Auxin Efflux.” Science 312 (5775): 914–918.
APA
Petrášek, J., Mravec, J., Bouchard, R., Blakeslee, J. J., Abas, M., Seifertová, D., Wiśniewska, J., et al. (2006). PIN proteins perform a rate-limiting function in cellular auxin efflux. SCIENCE, 312(5775), 914–918.
Vancouver
1.
Petrášek J, Mravec J, Bouchard R, Blakeslee JJ, Abas M, Seifertová D, et al. PIN proteins perform a rate-limiting function in cellular auxin efflux. SCIENCE. 2006;312(5775):914–8.
MLA
Petrášek, Jan, Jozef Mravec, Rodolphe Bouchard, et al. “PIN Proteins Perform a Rate-limiting Function in Cellular Auxin Efflux.” SCIENCE 312.5775 (2006): 914–918. Print.
@article{3202731,
  abstract     = {Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed ( PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport - related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.},
  author       = {Petr{\'a}\v{s}ek, Jan and Mravec, Jozef and Bouchard, Rodolphe and Blakeslee, Joshua J and Abas, Melinda and Seifertov{\'a}, Daniela and Wi\'{s}niewska, Justyna and Tadele, Zerihun and Kube\v{s}, Martin and \v{C}ovanov{\'a}, Milada and Dhonukshe, Pankaj and Sk\r{u}pa, Petr and Benkova, Eva and Perry, Lucie and K\v{r}e\v{c}ek, Pavel and Lee, Ok Ran and Fink, Gerald R and Geisler, Markus and Murphy, Angus S and Luschnig, Christian and Za\v{z}{\'i}malov{\'a}, Eva and Friml, Jiri},
  issn         = {0036-8075},
  journal      = {SCIENCE},
  keyword      = {TRANSGENIC PLANTS,ARABIDOPSIS-THALIANA,TRANSPORT,GRADIENTS,GENE,GRAVITROPISM,EXPRESSION,REGULATOR},
  language     = {eng},
  number       = {5775},
  pages        = {914--918},
  title        = {PIN proteins perform a rate-limiting function in cellular auxin efflux},
  url          = {http://dx.doi.org/10.1126/science.1123542},
  volume       = {312},
  year         = {2006},
}

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