Arabidopsis tubulin folding cofactor B interacts with α-tubulin in vivo
- Author
- Pankaj Dhonukshe (UGent) , Bastiaan OR Bargmann and Theodorus WJ Gadella
- Organization
- Abstract
- Microtubule biogenesis requires alpha beta tubulin dimers that are generated from alpha and beta tubulin following post-translational modification by several tubulin folding cofactors (TFCs). Here we report the isolation and characterization of Arabidopsis TFCB (AtTFCB). AtTFCB is expressed in all organs of Arabidopsis. The subcellular localization of AtTFCB is mainly cytosolic. AtTFCB-overexpressing cells have fewer microtubules compared with the controls. Multimode fluorescence resonance energy transfer (FRET) microscopy reveals a direct physical interaction of AtTFCB with alpha tubulin in living plant cells. We conclude that AtTFCB interacts with alpha tubulin in vivo and its overexpression reduces the number of microtubules.
- Keywords
- ENERGY-TRANSFER, PROTEIN, FRET MICROSCOPY, LIVING PLANT-CELLS, tubulin folding, microtubule, GFP, cofactor, FRET, THALIANA, PATHWAY, GENES
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-3202721
- MLA
- Dhonukshe, Pankaj, et al. “Arabidopsis Tubulin Folding Cofactor B Interacts with α-Tubulin in Vivo.” PLANT AND CELL PHYSIOLOGY, vol. 47, no. 10, 2006, pp. 1406–11, doi:10.1093/pcp/pcl001.
- APA
- Dhonukshe, P., Bargmann, B. O., & Gadella, T. W. (2006). Arabidopsis tubulin folding cofactor B interacts with α-tubulin in vivo. PLANT AND CELL PHYSIOLOGY, 47(10), 1406–1411. https://doi.org/10.1093/pcp/pcl001
- Chicago author-date
- Dhonukshe, Pankaj, Bastiaan OR Bargmann, and Theodorus WJ Gadella. 2006. “Arabidopsis Tubulin Folding Cofactor B Interacts with α-Tubulin in Vivo.” PLANT AND CELL PHYSIOLOGY 47 (10): 1406–11. https://doi.org/10.1093/pcp/pcl001.
- Chicago author-date (all authors)
- Dhonukshe, Pankaj, Bastiaan OR Bargmann, and Theodorus WJ Gadella. 2006. “Arabidopsis Tubulin Folding Cofactor B Interacts with α-Tubulin in Vivo.” PLANT AND CELL PHYSIOLOGY 47 (10): 1406–1411. doi:10.1093/pcp/pcl001.
- Vancouver
- 1.Dhonukshe P, Bargmann BO, Gadella TW. Arabidopsis tubulin folding cofactor B interacts with α-tubulin in vivo. PLANT AND CELL PHYSIOLOGY. 2006;47(10):1406–11.
- IEEE
- [1]P. Dhonukshe, B. O. Bargmann, and T. W. Gadella, “Arabidopsis tubulin folding cofactor B interacts with α-tubulin in vivo,” PLANT AND CELL PHYSIOLOGY, vol. 47, no. 10, pp. 1406–1411, 2006.
@article{3202721,
abstract = {{Microtubule biogenesis requires alpha beta tubulin dimers that are generated from alpha and beta tubulin following post-translational modification by several tubulin folding cofactors (TFCs). Here we report the isolation and characterization of Arabidopsis TFCB (AtTFCB). AtTFCB is expressed in all organs of Arabidopsis. The subcellular localization of AtTFCB is mainly cytosolic. AtTFCB-overexpressing cells have fewer microtubules compared with the controls. Multimode fluorescence resonance energy transfer (FRET) microscopy reveals a direct physical interaction of AtTFCB with alpha tubulin in living plant cells. We conclude that AtTFCB interacts with alpha tubulin in vivo and its overexpression reduces the number of microtubules.}},
author = {{Dhonukshe, Pankaj and Bargmann, Bastiaan OR and Gadella, Theodorus WJ}},
issn = {{0032-0781}},
journal = {{PLANT AND CELL PHYSIOLOGY}},
keywords = {{ENERGY-TRANSFER,PROTEIN,FRET MICROSCOPY,LIVING PLANT-CELLS,tubulin folding,microtubule,GFP,cofactor,FRET,THALIANA,PATHWAY,GENES}},
language = {{eng}},
number = {{10}},
pages = {{1406--1411}},
title = {{Arabidopsis tubulin folding cofactor B interacts with α-tubulin in vivo}},
url = {{http://dx.doi.org/10.1093/pcp/pcl001}},
volume = {{47}},
year = {{2006}},
}
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