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Subcellular trafficking of the Arabidopsis auxin influx carrier AUX1 uses a novel pathway distinct from PIN1

Jürgen Kleine-Vehn UGent, Pankaj Dhonukshe UGent, Ranjan Swarup, Malcolm Bennett and Jiri Friml (2006) PLANT CELL. 18(11). p.3171-3181
abstract
The directional flow of the plant hormone auxin mediates multiple developmental processes, including patterning and tropisms. Apical and basal plasma membrane localization of AUXIN-RESISTANT1 (AUX1) and PIN-FORMED1 (PIN1) auxin transport components underpins the directionality of intercellular auxin flow in Arabidopsis thaliana roots. Here, we examined the mechanism of polar trafficking of AUX1. Real-time live cell analysis along with subcellular markers revealed that AUX1 resides at the apical plasma membrane of protophloem cells and at highly dynamic subpopulations of Golgi apparatus and endosomes in all cell types. Plasma membrane and intracellular pools of AUX1 are interconnected by actin-dependent constitutive trafficking, which is not sensitive to the vesicle trafficking inhibitor brefeldin A. AUX1 subcellular dynamics are not influenced by the auxin influx inhibitor NOA but are blocked by the auxin efflux inhibitors TIBA and PBA. Furthermore, auxin transport inhibitors and interference with the sterol composition of membranes disrupt polar AUX1 distribution at the plasma membrane. Compared with PIN1 trafficking, AUX1 dynamics display different sensitivities to trafficking inhibitors and are independent of the endosomal trafficking regulator ARF GEF GNOM. Hence, AUX1 uses a novel trafficking pathway in plants that is distinct from PIN trafficking, providing an additional mechanism for the fine regulation of auxin transport.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GNOM ARF-GEF, LATERAL ROOT CAP, CELL POLARITY, EPITHELIAL-CELLS, COATED VESICLES, PERMEASE AUX1, PLANT-CELLS, BREFELDIN-A, TRANSPORT, PROTEIN
journal title
PLANT CELL
Plant Cell
volume
18
issue
11
pages
3171 - 3181
Web of Science type
Article
Web of Science id
000243093700025
JCR category
PLANT SCIENCES
JCR impact factor
9.868 (2006)
JCR rank
3/145 (2006)
JCR quartile
1 (2006)
ISSN
1040-4651
DOI
10.1105/tpc.106.042770
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3202714
handle
http://hdl.handle.net/1854/LU-3202714
date created
2013-04-26 13:41:05
date last changed
2016-12-19 15:43:05
@article{3202714,
  abstract     = {The directional flow of the plant hormone auxin mediates multiple developmental processes, including patterning and tropisms. Apical and basal plasma membrane localization of AUXIN-RESISTANT1 (AUX1) and PIN-FORMED1 (PIN1) auxin transport components underpins the directionality of intercellular auxin flow in Arabidopsis thaliana roots. Here, we examined the mechanism of polar trafficking of AUX1. Real-time live cell analysis along with subcellular markers revealed that AUX1 resides at the apical plasma membrane of protophloem cells and at highly dynamic subpopulations of Golgi apparatus and endosomes in all cell types. Plasma membrane and intracellular pools of AUX1 are interconnected by actin-dependent constitutive trafficking, which is not sensitive to the vesicle trafficking inhibitor brefeldin A. AUX1 subcellular dynamics are not influenced by the auxin influx inhibitor NOA but are blocked by the auxin efflux inhibitors TIBA and PBA. Furthermore, auxin transport inhibitors and interference with the sterol composition of membranes disrupt polar AUX1 distribution at the plasma membrane. Compared with PIN1 trafficking, AUX1 dynamics display different sensitivities to trafficking inhibitors and are independent of the endosomal trafficking regulator ARF GEF GNOM. Hence, AUX1 uses a novel trafficking pathway in plants that is distinct from PIN trafficking, providing an additional mechanism for the fine regulation of auxin transport.},
  author       = {Kleine-Vehn, J{\"u}rgen and Dhonukshe, Pankaj and Swarup, Ranjan and Bennett, Malcolm and Friml, Jiri},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {GNOM ARF-GEF,LATERAL ROOT CAP,CELL POLARITY,EPITHELIAL-CELLS,COATED VESICLES,PERMEASE AUX1,PLANT-CELLS,BREFELDIN-A,TRANSPORT,PROTEIN},
  language     = {eng},
  number       = {11},
  pages        = {3171--3181},
  title        = {Subcellular trafficking of the Arabidopsis auxin influx carrier AUX1 uses a novel pathway distinct from PIN1},
  url          = {http://dx.doi.org/10.1105/tpc.106.042770},
  volume       = {18},
  year         = {2006},
}

Chicago
Kleine-Vehn, Jürgen, Pankaj Dhonukshe, Ranjan Swarup, Malcolm Bennett, and Jiri Friml. 2006. “Subcellular Trafficking of the Arabidopsis Auxin Influx Carrier AUX1 Uses a Novel Pathway Distinct from PIN1.” Plant Cell 18 (11): 3171–3181.
APA
Kleine-Vehn, J., Dhonukshe, P., Swarup, R., Bennett, M., & Friml, J. (2006). Subcellular trafficking of the Arabidopsis auxin influx carrier AUX1 uses a novel pathway distinct from PIN1. PLANT CELL, 18(11), 3171–3181.
Vancouver
1.
Kleine-Vehn J, Dhonukshe P, Swarup R, Bennett M, Friml J. Subcellular trafficking of the Arabidopsis auxin influx carrier AUX1 uses a novel pathway distinct from PIN1. PLANT CELL. 2006;18(11):3171–81.
MLA
Kleine-Vehn, Jürgen, Pankaj Dhonukshe, Ranjan Swarup, et al. “Subcellular Trafficking of the Arabidopsis Auxin Influx Carrier AUX1 Uses a Novel Pathway Distinct from PIN1.” PLANT CELL 18.11 (2006): 3171–3181. Print.