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CLASP-mediated cortical microtubule organization guides PIN polarization axis

Klementina Kakar, Hongtao Zhang, Ben Scheres and Pankaj Dhonukshe UGent (2013) NATURE. 495(7442). p.529-533
abstract
Recent evidence indicates a correlation between orientation of the plant cortical microtubule cytoskeleton and localization of polar cargoes(1). However, the molecules and mechanisms that create this correlation have remained unknown. Here we show that, in Arabidopsis thaliana, the microtubule orientation regulators CLASP(2,3) and MAP65 (refs 3, 4) control the abundance of polarity regulator PINOID kinase(5,6) at the plasma membrane. By localized upregulation of clathrin-dependent endocytosis(7) at cortical microtubule- and clathrin-rich domains orthogonal to the axis of polarity, PINOID accelerates the removal of auxin transporter PIN proteins(8-10) from those sites. This mechanism links directional microtubule organization to the polar localization of auxin transporter PIN proteins, and clarifies how microtubule-enriched cell sides are kept distinct from polar delivery domains. Our results identify the molecular machinery that connects microtubule organization to the regulation of the axis of PIN polarization.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PLANT DEVELOPMENT, AUXIN-EFFLUX, ARABIDOPSIS ROOT, PLASMA-MEMBRANE, CELL-DIVISION, ENDOCYTOSIS, POLARITY, TRANSPORT, PROTEINS, LOCALIZATION
journal title
NATURE
Nature
volume
495
issue
7442
pages
529 - 533
Web of Science type
Article
Web of Science id
000316682800050
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
42.351 (2013)
JCR rank
1/55 (2013)
JCR quartile
1 (2013)
ISSN
0028-0836
DOI
10.1038/nature11980
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3202625
handle
http://hdl.handle.net/1854/LU-3202625
date created
2013-04-26 11:20:14
date last changed
2016-12-19 15:43:14
@article{3202625,
  abstract     = {Recent evidence indicates a correlation between orientation of the plant cortical microtubule cytoskeleton and localization of polar cargoes(1). However, the molecules and mechanisms that create this correlation have remained unknown. Here we show that, in Arabidopsis thaliana, the microtubule orientation regulators CLASP(2,3) and MAP65 (refs 3, 4) control the abundance of polarity regulator PINOID kinase(5,6) at the plasma membrane. By localized upregulation of clathrin-dependent endocytosis(7) at cortical microtubule- and clathrin-rich domains orthogonal to the axis of polarity, PINOID accelerates the removal of auxin transporter PIN proteins(8-10) from those sites. This mechanism links directional microtubule organization to the polar localization of auxin transporter PIN proteins, and clarifies how microtubule-enriched cell sides are kept distinct from polar delivery domains. Our results identify the molecular machinery that connects microtubule organization to the regulation of the axis of PIN polarization.},
  author       = {Kakar, Klementina and Zhang, Hongtao and Scheres, Ben and Dhonukshe, Pankaj},
  issn         = {0028-0836},
  journal      = {NATURE},
  keyword      = {PLANT DEVELOPMENT,AUXIN-EFFLUX,ARABIDOPSIS ROOT,PLASMA-MEMBRANE,CELL-DIVISION,ENDOCYTOSIS,POLARITY,TRANSPORT,PROTEINS,LOCALIZATION},
  language     = {eng},
  number       = {7442},
  pages        = {529--533},
  title        = {CLASP-mediated cortical microtubule organization guides PIN polarization axis},
  url          = {http://dx.doi.org/10.1038/nature11980},
  volume       = {495},
  year         = {2013},
}

Chicago
Kakar, Klementina, Hongtao Zhang, Ben Scheres, and Pankaj Dhonukshe. 2013. “CLASP-mediated Cortical Microtubule Organization Guides PIN Polarization Axis.” Nature 495 (7442): 529–533.
APA
Kakar, K., Zhang, H., Scheres, B., & Dhonukshe, P. (2013). CLASP-mediated cortical microtubule organization guides PIN polarization axis. NATURE, 495(7442), 529–533.
Vancouver
1.
Kakar K, Zhang H, Scheres B, Dhonukshe P. CLASP-mediated cortical microtubule organization guides PIN polarization axis. NATURE. 2013;495(7442):529–33.
MLA
Kakar, Klementina, Hongtao Zhang, Ben Scheres, et al. “CLASP-mediated Cortical Microtubule Organization Guides PIN Polarization Axis.” NATURE 495.7442 (2013): 529–533. Print.