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CLASP-mediated cortical microtubule organization guides PIN polarization axis

(2013) NATURE. 495(7442). p.529-533
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Abstract
Recent evidence indicates a correlation between orientation of the plant cortical microtubule cytoskeleton and localization of polar cargoes(1). However, the molecules and mechanisms that create this correlation have remained unknown. Here we show that, in Arabidopsis thaliana, the microtubule orientation regulators CLASP(2,3) and MAP65 (refs 3, 4) control the abundance of polarity regulator PINOID kinase(5,6) at the plasma membrane. By localized upregulation of clathrin-dependent endocytosis(7) at cortical microtubule- and clathrin-rich domains orthogonal to the axis of polarity, PINOID accelerates the removal of auxin transporter PIN proteins(8-10) from those sites. This mechanism links directional microtubule organization to the polar localization of auxin transporter PIN proteins, and clarifies how microtubule-enriched cell sides are kept distinct from polar delivery domains. Our results identify the molecular machinery that connects microtubule organization to the regulation of the axis of PIN polarization.
Keywords
PLANT DEVELOPMENT, AUXIN-EFFLUX, ARABIDOPSIS ROOT, PLASMA-MEMBRANE, CELL-DIVISION, ENDOCYTOSIS, POLARITY, TRANSPORT, PROTEINS, LOCALIZATION

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Citation

Please use this url to cite or link to this publication:

Chicago
Kakar, Klementina, Hongtao Zhang, Ben Scheres, and Pankaj Dhonukshe. 2013. “CLASP-mediated Cortical Microtubule Organization Guides PIN Polarization Axis.” Nature 495 (7442): 529–533.
APA
Kakar, K., Zhang, H., Scheres, B., & Dhonukshe, P. (2013). CLASP-mediated cortical microtubule organization guides PIN polarization axis. NATURE, 495(7442), 529–533.
Vancouver
1.
Kakar K, Zhang H, Scheres B, Dhonukshe P. CLASP-mediated cortical microtubule organization guides PIN polarization axis. NATURE. 2013;495(7442):529–33.
MLA
Kakar, Klementina, Hongtao Zhang, Ben Scheres, et al. “CLASP-mediated Cortical Microtubule Organization Guides PIN Polarization Axis.” NATURE 495.7442 (2013): 529–533. Print.
@article{3202625,
  abstract     = {Recent evidence indicates a correlation between orientation of the plant cortical microtubule cytoskeleton and localization of polar cargoes(1). However, the molecules and mechanisms that create this correlation have remained unknown. Here we show that, in Arabidopsis thaliana, the microtubule orientation regulators CLASP(2,3) and MAP65 (refs 3, 4) control the abundance of polarity regulator PINOID kinase(5,6) at the plasma membrane. By localized upregulation of clathrin-dependent endocytosis(7) at cortical microtubule- and clathrin-rich domains orthogonal to the axis of polarity, PINOID accelerates the removal of auxin transporter PIN proteins(8-10) from those sites. This mechanism links directional microtubule organization to the polar localization of auxin transporter PIN proteins, and clarifies how microtubule-enriched cell sides are kept distinct from polar delivery domains. Our results identify the molecular machinery that connects microtubule organization to the regulation of the axis of PIN polarization.},
  author       = {Kakar, Klementina and Zhang, Hongtao and Scheres, Ben and Dhonukshe, Pankaj},
  issn         = {0028-0836},
  journal      = {NATURE},
  keyword      = {PLANT DEVELOPMENT,AUXIN-EFFLUX,ARABIDOPSIS ROOT,PLASMA-MEMBRANE,CELL-DIVISION,ENDOCYTOSIS,POLARITY,TRANSPORT,PROTEINS,LOCALIZATION},
  language     = {eng},
  number       = {7442},
  pages        = {529--533},
  title        = {CLASP-mediated cortical microtubule organization guides PIN polarization axis},
  url          = {http://dx.doi.org/10.1038/nature11980},
  volume       = {495},
  year         = {2013},
}

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