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SCFTIR1/AFB-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism

Pawel Radoslaw Baster (UGent) , Stephanie Robert (UGent) , Jürgen Kleine-Vehn (UGent) , Steffen Vanneste (UGent) , Urszula Kania (UGent) , Wim Grunewald (UGent) , Bert De Rybel (UGent) , Tom Beeckman (UGent) and Jiri Friml (UGent)
(2013) EMBO JOURNAL. 32(2). p.260-274
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Abstract
The distribution of the phytohormone auxin regulates many aspects of plant development including growth response to gravity. Gravitropic root curvature involves coordinated and asymmetric cell elongation between the lower and upper side of the root, mediated by differential cellular auxin levels. The asymmetry in the auxin distribution is established and maintained by a spatio-temporal regulation of the PIN-FORMED (PIN) auxin transporter activity. We provide novel insights into the complex regulation of PIN abundance and activity during root gravitropism. We show that PIN2 turnover is differentially regulated on the upper and lower side of gravistimulated roots by distinct but partially overlapping auxin feedback mechanisms. In addition to regulating transcription and clathrin-mediated internalization, auxin also controls PIN abundance at the plasma membrane by promoting their vacuolar targeting and degradation. This effect of elevated auxin levels requires the activity of SKP-Cullin-F-box(TIR1/AFB) (SCFTIR1/AFB)-dependent pathway. Importantly, also suboptimal auxin levels mediate PIN degradation utilizing the same signalling pathway. These feedback mechanisms are functionally important during gravitropic response and ensure fine-tuning of auxin fluxes for maintaining as well as terminating asymmetric growth.
Keywords
PLANT DEVELOPMENT, PLASMA-MEMBRANE, ARABIDOPSIS-THALIANA, BOX PROTEIN TIR1, FACTOR GENE FAMILY, YUCCA FLAVIN MONOOXYGENASES, EFFLUX CARRIER POLARITY, PIN, gravitropism, degradation, auxin, BINDING-PROTEIN, DEPENDENT ENDOCYTOSIS, UBIQUITIN LIGASE

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Citation

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Chicago
Baster, Pawel Radoslaw, Stephanie Robert, Jürgen Kleine-Vehn, Steffen Vanneste, Urszula Kania, Wim Grunewald, Bert De Rybel, Tom Beeckman, and Jiri Friml. 2013. “SCFTIR1/AFB-auxin Signalling Regulates PIN Vacuolar Trafficking and Auxin Fluxes During Root Gravitropism.” Embo Journal 32 (2): 260–274.
APA
Baster, P. R., Robert, S., Kleine-Vehn, J., Vanneste, S., Kania, U., Grunewald, W., De Rybel, B., et al. (2013). SCFTIR1/AFB-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism. EMBO JOURNAL, 32(2), 260–274.
Vancouver
1.
Baster PR, Robert S, Kleine-Vehn J, Vanneste S, Kania U, Grunewald W, et al. SCFTIR1/AFB-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism. EMBO JOURNAL. 2013;32(2):260–74.
MLA
Baster, Pawel Radoslaw, Stephanie Robert, Jürgen Kleine-Vehn, et al. “SCFTIR1/AFB-auxin Signalling Regulates PIN Vacuolar Trafficking and Auxin Fluxes During Root Gravitropism.” EMBO JOURNAL 32.2 (2013): 260–274. Print.
@article{3202591,
  abstract     = {The distribution of the phytohormone auxin regulates many aspects of plant development including growth response to gravity. Gravitropic root curvature involves coordinated and asymmetric cell elongation between the lower and upper side of the root, mediated by differential cellular auxin levels. The asymmetry in the auxin distribution is established and maintained by a spatio-temporal regulation of the PIN-FORMED (PIN) auxin transporter activity. We provide novel insights into the complex regulation of PIN abundance and activity during root gravitropism. We show that PIN2 turnover is differentially regulated on the upper and lower side of gravistimulated roots by distinct but partially overlapping auxin feedback mechanisms. In addition to regulating transcription and clathrin-mediated internalization, auxin also controls PIN abundance at the plasma membrane by promoting their vacuolar targeting and degradation. This effect of elevated auxin levels requires the activity of SKP-Cullin-F-box(TIR1/AFB) (SCFTIR1/AFB)-dependent pathway. Importantly, also suboptimal auxin levels mediate PIN degradation utilizing the same signalling pathway. These feedback mechanisms are functionally important during gravitropic response and ensure fine-tuning of auxin fluxes for maintaining as well as terminating asymmetric growth.},
  author       = {Baster, Pawel Radoslaw and Robert, Stephanie and Kleine-Vehn, J{\"u}rgen and Vanneste, Steffen and Kania, Urszula and Grunewald, Wim and De Rybel, Bert and Beeckman, Tom and Friml, Jiri},
  issn         = {0261-4189},
  journal      = {EMBO JOURNAL},
  keyword      = {PLANT DEVELOPMENT,PLASMA-MEMBRANE,ARABIDOPSIS-THALIANA,BOX PROTEIN TIR1,FACTOR GENE FAMILY,YUCCA FLAVIN MONOOXYGENASES,EFFLUX CARRIER POLARITY,PIN,gravitropism,degradation,auxin,BINDING-PROTEIN,DEPENDENT ENDOCYTOSIS,UBIQUITIN LIGASE},
  language     = {eng},
  number       = {2},
  pages        = {260--274},
  title        = {SCFTIR1/AFB-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism},
  url          = {http://dx.doi.org/10.1038/emboj.2012.310},
  volume       = {32},
  year         = {2013},
}

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