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Dynamics of an oligotrophic bacterial aquifer community during contact with a groundwater plume contaminated with benzene toluene, ethylbenzene, and xylenes: an in situ mesocosm study

Barbara Hendrickx, Winnie Dejonghe, Wesley Boënne, Maria Brennerova, Miroslav Cernik, Tomas Lederer, Margareta Bucheli-Witschel, Leen Bastiaens, Willy Verstraete UGent and Eva M Top, et al. (2005) APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 71(7). p.3815-3825
abstract
An in situ mesocosm system was designed to monitor the in situ dynamics of the microbial community in polluted aquifers. The mesocosm system consists of a permeable membrane pocket filled with aquifer material and placed within a polypropylene holder, which is inserted below groundwater level in a monitoring well. After a specific time period, the microcosm is recovered from the well and its bacterial community is analyzed. Using this system, we examined the effect of benzene, toluene, ethylbenzene, and xylene (BTEX) contamination on the response of an aquifer bacterial community by denaturing gradient gel electrophoresis analysis of PCR-amplified 16S rRNA genes and PCR detection of BTEX degradation genes. Mesocosms were filled with nonsterile or sterile aquifer material derived from an uncontaminated area and positioned in a well located in either the uncontaminated area or a nearby contaminated area. In the contaminated area, the bacterial community in the microcosms rapidly evolved into a stable community identical to that in the adjacent aquifer but different from that in the uncontaminated area. At the contaminated location, bacteria with tmoA- and xylM/xylE1-like BTEX catabolic genotypes colonized the aquifer, while at the uncontaminated location only tmoA-like genotypes were detected. The communities in the mesocosms and in the aquifer adjacent to the wells in the contaminated area consisted mainly of Proteobacteria. At the uncontaminated location, Actinobacteria and Proteobacteria were found. Our results indicate that communities with long-term stability in their structures follow the contamination plume and rapidly colonize downstream areas upon contamination.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
AROMATIC-HYDROCARBONS, 16S RIBOSOMAL-RNA, PSEUDOMONAS-PUTIDA, HYPOXIC ENVIRONMENTS, MICROBIAL DIVERSITY, DEGRADING BACTERIA, ORGANIC-SOLVENTS, GENE DIVERSITY, SOIL, BIOREMEDIATION
journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Appl. Environ. Microbiol.
volume
71
issue
7
pages
3815-3825 pages
Web of Science type
Article
Web of Science id
000230445700054
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.818 (2005)
JCR rank
21/135 (2005)
JCR quartile
1 (2005)
ISSN
0099-2240
DOI
10.1128/AEM.71.7.3815-3825.2005
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
320200
handle
http://hdl.handle.net/1854/LU-320200
date created
2005-08-09 16:29:00
date last changed
2010-04-08 09:32:04
@article{320200,
  abstract     = {An in situ mesocosm system was designed to monitor the in situ dynamics of the microbial community in polluted aquifers. The mesocosm system consists of a permeable membrane pocket filled with aquifer material and placed within a polypropylene holder, which is inserted below groundwater level in a monitoring well. After a specific time period, the microcosm is recovered from the well and its bacterial community is analyzed. Using this system, we examined the effect of benzene, toluene, ethylbenzene, and xylene (BTEX) contamination on the response of an aquifer bacterial community by denaturing gradient gel electrophoresis analysis of PCR-amplified 16S rRNA genes and PCR detection of BTEX degradation genes. Mesocosms were filled with nonsterile or sterile aquifer material derived from an uncontaminated area and positioned in a well located in either the uncontaminated area or a nearby contaminated area. In the contaminated area, the bacterial community in the microcosms rapidly evolved into a stable community identical to that in the adjacent aquifer but different from that in the uncontaminated area. At the contaminated location, bacteria with tmoA- and xylM/xylE1-like BTEX catabolic genotypes colonized the aquifer, while at the uncontaminated location only tmoA-like genotypes were detected. The communities in the mesocosms and in the aquifer adjacent to the wells in the contaminated area consisted mainly of Proteobacteria. At the uncontaminated location, Actinobacteria and Proteobacteria were found. Our results indicate that communities with long-term stability in their structures follow the contamination plume and rapidly colonize downstream areas upon contamination.},
  author       = {Hendrickx, Barbara and Dejonghe, Winnie and Bo{\"e}nne, Wesley and Brennerova, Maria and Cernik, Miroslav and Lederer, Tomas and Bucheli-Witschel, Margareta and Bastiaens, Leen and Verstraete, Willy and Top, Eva M and Diels, Ludo and Springael, Dirk},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {AROMATIC-HYDROCARBONS,16S RIBOSOMAL-RNA,PSEUDOMONAS-PUTIDA,HYPOXIC ENVIRONMENTS,MICROBIAL DIVERSITY,DEGRADING BACTERIA,ORGANIC-SOLVENTS,GENE DIVERSITY,SOIL,BIOREMEDIATION},
  language     = {eng},
  number       = {7},
  pages        = {3815--3825},
  title        = {Dynamics of an oligotrophic bacterial aquifer community during contact with a groundwater plume contaminated with benzene toluene, ethylbenzene, and xylenes: an in situ mesocosm study},
  url          = {http://dx.doi.org/10.1128/AEM.71.7.3815-3825.2005},
  volume       = {71},
  year         = {2005},
}

Chicago
Hendrickx, Barbara, Winnie Dejonghe, Wesley Boënne, Maria Brennerova, Miroslav Cernik, Tomas Lederer, Margareta Bucheli-Witschel, et al. 2005. “Dynamics of an Oligotrophic Bacterial Aquifer Community During Contact with a Groundwater Plume Contaminated with Benzene Toluene, Ethylbenzene, and Xylenes: An in Situ Mesocosm Study.” Applied and Environmental Microbiology 71 (7): 3815–3825.
APA
Hendrickx, B., Dejonghe, W., Boënne, W., Brennerova, M., Cernik, M., Lederer, T., Bucheli-Witschel, M., et al. (2005). Dynamics of an oligotrophic bacterial aquifer community during contact with a groundwater plume contaminated with benzene toluene, ethylbenzene, and xylenes: an in situ mesocosm study. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(7), 3815–3825.
Vancouver
1.
Hendrickx B, Dejonghe W, Boënne W, Brennerova M, Cernik M, Lederer T, et al. Dynamics of an oligotrophic bacterial aquifer community during contact with a groundwater plume contaminated with benzene toluene, ethylbenzene, and xylenes: an in situ mesocosm study. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2005;71(7):3815–25.
MLA
Hendrickx, Barbara, Winnie Dejonghe, Wesley Boënne, et al. “Dynamics of an Oligotrophic Bacterial Aquifer Community During Contact with a Groundwater Plume Contaminated with Benzene Toluene, Ethylbenzene, and Xylenes: An in Situ Mesocosm Study.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 71.7 (2005): 3815–3825. Print.