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Characterization of methicillin-resistant non-Staphylococcus aureus staphylococci carriage isolates from different bovine populations

Wannes Vanderhaeghen, Stien Vandendriessche, Florence Crombé UGent, Stéphanie Nemeghaire, Marc Dispas, Olivier Denis, Katleen Hermans UGent, Freddy Haesebrouck UGent and Patrick Butaye UGent (2013) JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. 68(2). p.300-307
abstract
This study aimed at investigating bovine non-Staphylococcus aureus staphylococci for their role as a potential reservoir for methicillin resistance. Nasal swab samples were collected from 150 veal calves on 15 veal farms, 100 dairy cows on 10 dairy farms and 100 beef cows on 10 beef farms. Suspected staphylococcal isolates were investigated by PCR for the presence of the classic mecA and mecA(LGA251). Methicillin-resistant non-S. aureus staphylococci (MRNAS) were genotypically identified and were characterized by broth microdilution antimicrobial susceptibility testing and staphylococcal cassette chromosome mec (SCCmec) typing. The MRNAS (n101) carriage rate was estimated as 30.29 (95 CI 6.1474.28) in veal calves, 13.1 (95 CI 1.2863.72) in dairy cows and 24.8 (95 CI 11.9744.42) in beef cows. Carriage rates were not significantly different between the three populations (P0.05). mecA(LGA251) was not detected. Most (n80) MRNAS were identified as Staphylococcus sciuri, Staphylococcus lentus or Staphylococcus fleurettii. Resistance to aminoglycosides, macrolidelincosamidestreptogramin antimicrobials, tetracycline and ciprofloxacin was frequently detected. Two linezolid-resistant MRNAS from veal calves carried the multidrug-resistance gene cfr. SCCmec cassettes of type III predominated (n46); another 40 SCCmec cassettes harboured a class A mec complex without identifiable ccr complex; type IVa, type V and several other non-typeable cassettes were detected in low frequencies, especially in methicillin-resistant Staphylococcus epidermidis. The SCCmec types predominating in bovine MRNAS differ from those mostly detected in livestock-associated methicillin-resistant S. aureus strains. Yet, the detection of cfr and the high level of other antimicrobial resistances suggest a potentially important role of bovine MRNAS as a reservoir for resistance determinants other than SCCmec.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SCCmec, Staphylococcus sciuri, cattle, coagulase-negative staphylococci, CASSETTE CHROMOSOME MEC, COAGULASE-NEGATIVE STAPHYLOCOCCI, MULTIRESISTANCE GENE CFR, ANIMAL ORIGIN, 1ST REPORT, SCCMEC, IDENTIFICATION, SEQUENCE, ELEMENTS, PLASMID
journal title
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
J. Antimicrob. Chemother.
volume
68
issue
2
pages
300 - 307
Web of Science type
Article
Web of Science id
000313652700007
JCR category
PHARMACOLOGY & PHARMACY
JCR impact factor
5.439 (2013)
JCR rank
17/256 (2013)
JCR quartile
1 (2013)
ISSN
0305-7453
DOI
10.1093/jac/dks403
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3199404
handle
http://hdl.handle.net/1854/LU-3199404
date created
2013-04-23 16:24:07
date last changed
2016-12-19 15:43:11
@article{3199404,
  abstract     = {This study aimed at investigating bovine non-Staphylococcus aureus staphylococci for their role as a potential reservoir for methicillin resistance. 
Nasal swab samples were collected from 150 veal calves on 15 veal farms, 100 dairy cows on 10 dairy farms and 100 beef cows on 10 beef farms. Suspected staphylococcal isolates were investigated by PCR for the presence of the classic mecA and mecA(LGA251). Methicillin-resistant non-S. aureus staphylococci (MRNAS) were genotypically identified and were characterized by broth microdilution antimicrobial susceptibility testing and staphylococcal cassette chromosome mec (SCCmec) typing. 
The MRNAS (n101) carriage rate was estimated as 30.29 (95 CI 6.1474.28) in veal calves, 13.1 (95 CI 1.2863.72) in dairy cows and 24.8 (95 CI 11.9744.42) in beef cows. Carriage rates were not significantly different between the three populations (P0.05). mecA(LGA251) was not detected. Most (n80) MRNAS were identified as Staphylococcus sciuri, Staphylococcus lentus or Staphylococcus fleurettii. Resistance to aminoglycosides, macrolidelincosamidestreptogramin antimicrobials, tetracycline and ciprofloxacin was frequently detected. Two linezolid-resistant MRNAS from veal calves carried the multidrug-resistance gene cfr. SCCmec cassettes of type III predominated (n46); another 40 SCCmec cassettes harboured a class A mec complex without identifiable ccr complex; type IVa, type V and several other non-typeable cassettes were detected in low frequencies, especially in methicillin-resistant Staphylococcus epidermidis. 
The SCCmec types predominating in bovine MRNAS differ from those mostly detected in livestock-associated methicillin-resistant S. aureus strains. Yet, the detection of cfr and the high level of other antimicrobial resistances suggest a potentially important role of bovine MRNAS as a reservoir for resistance determinants other than SCCmec.},
  author       = {Vanderhaeghen, Wannes and Vandendriessche, Stien and Cromb{\'e}, Florence and Nemeghaire, St{\'e}phanie and Dispas, Marc and Denis, Olivier and Hermans, Katleen and Haesebrouck, Freddy and Butaye, Patrick},
  issn         = {0305-7453},
  journal      = {JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY},
  keyword      = {SCCmec,Staphylococcus sciuri,cattle,coagulase-negative staphylococci,CASSETTE CHROMOSOME MEC,COAGULASE-NEGATIVE STAPHYLOCOCCI,MULTIRESISTANCE GENE CFR,ANIMAL ORIGIN,1ST REPORT,SCCMEC,IDENTIFICATION,SEQUENCE,ELEMENTS,PLASMID},
  language     = {eng},
  number       = {2},
  pages        = {300--307},
  title        = {Characterization of methicillin-resistant non-Staphylococcus aureus staphylococci carriage isolates from different bovine populations},
  url          = {http://dx.doi.org/10.1093/jac/dks403},
  volume       = {68},
  year         = {2013},
}

Chicago
Vanderhaeghen, Wannes, Stien Vandendriessche, Florence Crombé, Stéphanie Nemeghaire, Marc Dispas, Olivier Denis, Katleen Hermans, Freddy Haesebrouck, and Patrick Butaye. 2013. “Characterization of Methicillin-resistant non-Staphylococcus Aureus Staphylococci Carriage Isolates from Different Bovine Populations.” Journal of Antimicrobial Chemotherapy 68 (2): 300–307.
APA
Vanderhaeghen, W., Vandendriessche, S., Crombé, F., Nemeghaire, S., Dispas, M., Denis, O., Hermans, K., et al. (2013). Characterization of methicillin-resistant non-Staphylococcus aureus staphylococci carriage isolates from different bovine populations. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 68(2), 300–307.
Vancouver
1.
Vanderhaeghen W, Vandendriessche S, Crombé F, Nemeghaire S, Dispas M, Denis O, et al. Characterization of methicillin-resistant non-Staphylococcus aureus staphylococci carriage isolates from different bovine populations. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. 2013;68(2):300–7.
MLA
Vanderhaeghen, Wannes, Stien Vandendriessche, Florence Crombé, et al. “Characterization of Methicillin-resistant non-Staphylococcus Aureus Staphylococci Carriage Isolates from Different Bovine Populations.” JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 68.2 (2013): 300–307. Print.