Advanced search
1 file | 381.79 KB

Multilocus sequence typing of the porcine and human gastric pathogen Helicobacter suis

Jungang Liang (UGent) , Richard Ducatelle (UGent) , Frank Pasmans (UGent) , Annemieke Smet (UGent) , Freddy Haesebrouck (UGent) and Bram Flahou (UGent)
Author
Organization
Abstract
Helicobacter suis is a Gram-negative bacterium colonizing the majority of pigs, in which it causes gastritis and decreased daily weight gain. H. suis is also the most prevalent gastric non-Helicobacter pylori Helicobacter species in humans, capable of causing gastric disorders. To gain insight into the genetic diversity of porcine and human H. suis strains, a multilocus sequence typing (MLST) method was developed. In a preliminary study, 7 housekeeping genes (atpA, efp, mutY, ppa, trpC, ureI, and yphC) of 10 H. suis isolates cultured in vitro were investigated as MLST candidates. All genes, except the ureI gene, which was replaced by part of the ureAB gene cluster of H. suis, displayed several variable nucleotide sites. Subsequently, internal gene fragments, ranging from 379 to 732 bp and comprising several variable nucleotide sites, were selected. For validation of the developed MLST technique, gastric tissue from 17 H. suis-positive pigs from 4 different herds and from 1 H. suis-infected human patient was used for direct, culture-independent strain typing of H. suis. In addition to the 10 unique sequence types (STs) among the 10 isolates grown in vitro, 15 additional STs could be assigned. Individual animals were colonized by only 1 H. suis strain, whereas multiple H. suis strains were present in all herds tested, revealing that H. suis is a genetically diverse bacterial species. The human H. suis strain showed a very close relationship to porcine strains. In conclusion, the developed MLST scheme may prove useful for direct, culture-independent typing of porcine and human H. suis strains.
Keywords
SWINE, MUCOSA, ORGANISMS, SAMPLES, INFECTION, IDENTIFICATION, PREVALENCE, POPULATION, PIGS, PYLORI

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 381.79 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Liang, Jungang, Richard Ducatelle, Frank Pasmans, Annemieke Smet, Freddy Haesebrouck, and Bram Flahou. 2013. “Multilocus Sequence Typing of the Porcine and Human Gastric Pathogen Helicobacter Suis.” Journal of Clinical Microbiology 51 (3): 920–926.
APA
Liang, J., Ducatelle, R., Pasmans, F., Smet, A., Haesebrouck, F., & Flahou, B. (2013). Multilocus sequence typing of the porcine and human gastric pathogen Helicobacter suis. JOURNAL OF CLINICAL MICROBIOLOGY, 51(3), 920–926.
Vancouver
1.
Liang J, Ducatelle R, Pasmans F, Smet A, Haesebrouck F, Flahou B. Multilocus sequence typing of the porcine and human gastric pathogen Helicobacter suis. JOURNAL OF CLINICAL MICROBIOLOGY. 2013;51(3):920–6.
MLA
Liang, Jungang, Richard Ducatelle, Frank Pasmans, et al. “Multilocus Sequence Typing of the Porcine and Human Gastric Pathogen Helicobacter Suis.” JOURNAL OF CLINICAL MICROBIOLOGY 51.3 (2013): 920–926. Print.
@article{3199384,
  abstract     = {Helicobacter suis is a Gram-negative bacterium colonizing the majority of pigs, in which it causes gastritis and decreased daily weight gain. H. suis is also the most prevalent gastric non-Helicobacter pylori Helicobacter species in humans, capable of causing gastric disorders. To gain insight into the genetic diversity of porcine and human H. suis strains, a multilocus sequence typing (MLST) method was developed. In a preliminary study, 7 housekeeping genes (atpA, efp, mutY, ppa, trpC, ureI, and yphC) of 10 H. suis isolates cultured in vitro were investigated as MLST candidates. All genes, except the ureI gene, which was replaced by part of the ureAB gene cluster of H. suis, displayed several variable nucleotide sites. Subsequently, internal gene fragments, ranging from 379 to 732 bp and comprising several variable nucleotide sites, were selected. For validation of the developed MLST technique, gastric tissue from 17 H. suis-positive pigs from 4 different herds and from 1 H. suis-infected human patient was used for direct, culture-independent strain typing of H. suis. In addition to the 10 unique sequence types (STs) among the 10 isolates grown in vitro, 15 additional STs could be assigned. Individual animals were colonized by only 1 H. suis strain, whereas multiple H. suis strains were present in all herds tested, revealing that H. suis is a genetically diverse bacterial species. The human H. suis strain showed a very close relationship to porcine strains. In conclusion, the developed MLST scheme may prove useful for direct, culture-independent typing of porcine and human H. suis strains.},
  author       = {Liang, Jungang and Ducatelle, Richard and Pasmans, Frank and Smet, Annemieke and Haesebrouck, Freddy and Flahou, Bram},
  issn         = {0095-1137},
  journal      = {JOURNAL OF CLINICAL MICROBIOLOGY},
  keyword      = {SWINE,MUCOSA,ORGANISMS,SAMPLES,INFECTION,IDENTIFICATION,PREVALENCE,POPULATION,PIGS,PYLORI},
  language     = {eng},
  number       = {3},
  pages        = {920--926},
  title        = {Multilocus sequence typing of the porcine and human gastric pathogen Helicobacter suis},
  url          = {http://dx.doi.org/10.1128/JCM.02399-12},
  volume       = {51},
  year         = {2013},
}

Altmetric
View in Altmetric
Web of Science
Times cited: