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Concurrent overactivation of the cytosolic glutamine synthetase and the GABA-shunt in the ABA-deficient sitiens mutant of tomato leads to resistance against Botrytis cinerea

Hamed Seifi (UGent) , Katrien Curvers (UGent) , David De Vleesschauwer (UGent) , Ilse Delaere (UGent) , Aziz Aziz and Monica Höfte (UGent)
(2013) NEW PHYTOLOGIST. 199(2). p.490-504
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Abstract
Deficiency of abscisic acid in the sitiens mutant of tomato culminates in increased resistance to Botrytis cinerea through a rapid epidermal hypersensitive response (HR) and associated phenylpropanoid-pathway-derived cell wall fortifications. This study focused on understanding the role of primary carbon/nitrogen (C/N) metabolism in the resistance response of sitiens to B. cinerea. how alterations in C/N metabolism are linked with the HR-mediated epidermal arrest of the pathogen, has been also investigated. Temporal alterations in the GABA shunt, GS/GOGAT cycle and phenylpropanoid pathway were transcriptionally, enzymatically and metabolically monitored in both wild-type and sitiens plants. Virus induced gene silencing, microscopic analyses and pharmacological assays were used to further confirm the data. Our results on the sitiens-B. cinerea interaction favor a model in which cell viability in the cells surrounding the invaded tissue is maintained by a constant replenishment of the TCA cycle through overactivation of the GS/GOGAT cycle and the GABA shunt, resulting in resistance via both tightly controlling the defense-associated HR and slowing down the pathogen-induced senescence. Collectively, this study shows that maintaining cell viability via alterations in host C/N metabolism plays a vital role in the resistance response against necrotrophic pathogens.
Keywords
central C/N metabolism, Botrytis cinerea, Abscisic acid, senescence, necrotrophic interaction, GABA shunt, PHENYLALANINE AMMONIA-LYASE, Solanum lycopersicum, GS/GOGAT cycle, GAMMA-AMINOBUTYRIC-ACID, SUCCINIC-SEMIALDEHYDE-DEHYDROGENASE, HYPERSENSITIVE RESPONSE, IN-VIVO, DIFFERENTIAL REGULATION, NITROGEN MOBILIZATION, ARABIDOPSIS-THALIANA, PRIMARY METABOLISM, DEFENSE RESPONSES

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MLA
Seifi, Hamed, Katrien Curvers, David De Vleesschauwer, et al. “Concurrent Overactivation of the Cytosolic Glutamine Synthetase and the GABA-shunt in the ABA-deficient Sitiens Mutant of Tomato Leads to Resistance Against Botrytis Cinerea.” NEW PHYTOLOGIST 199.2 (2013): 490–504. Print.
APA
Seifi, H., Curvers, K., De Vleesschauwer, D., Delaere, I., Aziz, A., & Höfte, M. (2013). Concurrent overactivation of the cytosolic glutamine synthetase and the GABA-shunt in the ABA-deficient sitiens mutant of tomato leads to resistance against Botrytis cinerea. NEW PHYTOLOGIST, 199(2), 490–504.
Chicago author-date
Seifi, Hamed, Katrien Curvers, David De Vleesschauwer, Ilse Delaere, Aziz Aziz, and Monica Höfte. 2013. “Concurrent Overactivation of the Cytosolic Glutamine Synthetase and the GABA-shunt in the ABA-deficient Sitiens Mutant of Tomato Leads to Resistance Against Botrytis Cinerea.” New Phytologist 199 (2): 490–504.
Chicago author-date (all authors)
Seifi, Hamed, Katrien Curvers, David De Vleesschauwer, Ilse Delaere, Aziz Aziz, and Monica Höfte. 2013. “Concurrent Overactivation of the Cytosolic Glutamine Synthetase and the GABA-shunt in the ABA-deficient Sitiens Mutant of Tomato Leads to Resistance Against Botrytis Cinerea.” New Phytologist 199 (2): 490–504.
Vancouver
1.
Seifi H, Curvers K, De Vleesschauwer D, Delaere I, Aziz A, Höfte M. Concurrent overactivation of the cytosolic glutamine synthetase and the GABA-shunt in the ABA-deficient sitiens mutant of tomato leads to resistance against Botrytis cinerea. NEW PHYTOLOGIST. 2013;199(2):490–504.
IEEE
[1]
H. Seifi, K. Curvers, D. De Vleesschauwer, I. Delaere, A. Aziz, and M. Höfte, “Concurrent overactivation of the cytosolic glutamine synthetase and the GABA-shunt in the ABA-deficient sitiens mutant of tomato leads to resistance against Botrytis cinerea,” NEW PHYTOLOGIST, vol. 199, no. 2, pp. 490–504, 2013.
@article{3191478,
  abstract     = {{Deficiency of abscisic acid in the sitiens mutant of tomato culminates in increased resistance to Botrytis cinerea through a rapid epidermal hypersensitive response (HR) and associated phenylpropanoid-pathway-derived cell wall fortifications. This study focused on understanding the role of primary carbon/nitrogen (C/N) metabolism in the resistance response of sitiens to B. cinerea. how alterations in C/N metabolism are linked with the HR-mediated epidermal arrest of the pathogen, has been also investigated.
Temporal alterations in the GABA shunt, GS/GOGAT cycle and phenylpropanoid pathway were transcriptionally, enzymatically and metabolically monitored in both wild-type and sitiens plants. Virus induced gene silencing, microscopic analyses and pharmacological assays were used to further confirm the data.
Our results on the sitiens-B. cinerea interaction favor a model in which cell viability in the cells surrounding the invaded tissue is maintained by a constant replenishment of the TCA cycle through overactivation of the GS/GOGAT cycle and the GABA shunt, resulting in resistance via both tightly controlling the defense-associated HR and slowing down the pathogen-induced senescence.
Collectively, this study shows that maintaining cell viability via alterations in host C/N metabolism plays a vital role in the resistance response against necrotrophic pathogens.}},
  author       = {{Seifi, Hamed and Curvers, Katrien and De Vleesschauwer, David and Delaere, Ilse and Aziz, Aziz and Höfte, Monica}},
  issn         = {{0028-646X}},
  journal      = {{NEW PHYTOLOGIST}},
  keywords     = {{central C/N metabolism,Botrytis cinerea,Abscisic acid,senescence,necrotrophic interaction,GABA shunt,PHENYLALANINE AMMONIA-LYASE,Solanum lycopersicum,GS/GOGAT cycle,GAMMA-AMINOBUTYRIC-ACID,SUCCINIC-SEMIALDEHYDE-DEHYDROGENASE,HYPERSENSITIVE RESPONSE,IN-VIVO,DIFFERENTIAL REGULATION,NITROGEN MOBILIZATION,ARABIDOPSIS-THALIANA,PRIMARY METABOLISM,DEFENSE RESPONSES}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{490--504}},
  title        = {{Concurrent overactivation of the cytosolic glutamine synthetase and the GABA-shunt in the ABA-deficient sitiens mutant of tomato leads to resistance against Botrytis cinerea}},
  url          = {{http://dx.doi.org/10.1111/nph.12283}},
  volume       = {{199}},
  year         = {{2013}},
}

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