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AUREOCHROME1a-mediated induction of the diatom-specific cyclin dsCYC2 controls the onset of cell division in diatoms (Phaeodactylum tricornutum)

(2013) PLANT CELL. 25(1). p.215-228
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Cell division in photosynthetic organisms is tightly regulated by light. Although the light dependency of the onset of the cell cycle has been well characterized in various phototrophs, little is known about the cellular signaling cascades connecting light perception to cell cycle activation and progression. Here, we demonstrate that diatom-specific cyclin 2 (dsCYC2) in Phaeodactylum tricornutum displays a transcriptional peak within 15 min after light exposure, long before the onset of cell division. The product of dsCYC2 binds to the cyclin-dependent kinase CDKA1 and can complement G1 cyclin-deficient yeast. Consistent with the role of dsCYC2 in controlling a G1-to-S light-dependent cell cycle checkpoint, dsCYC2 silencing decreases the rate of cell division in diatoms exposed to light-dark cycles but not to constant light. Transcriptional induction of dsCYC2 is triggered by blue light in a fluence rate-dependent manner. Consistent with this, dsCYC2 is a transcriptional target of the blue light sensor AUREOCHROME1a, which functions synergistically with the basic leucine zipper (bZIP) transcription factor bZIP10 to induce dsCYC2 transcription. The functional characterization of a cyclin whose transcription is controlled by light and whose activity connects light signaling to cell cycle progression contributes significantly to our understanding of the molecular mechanisms underlying light-dependent cell cycle onset in diatoms.
Keywords
PLANT, YEAST, HIGH LIGHT, SACCHAROMYCES-CEREVISIAE, MARINE-PHYTOPLANKTON, GENE-EXPRESSION, PHOTOPROTECTION, PROGRESSION, PHOTOSYNTHESIS, PROTEIN

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Citation

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Chicago
Huysman, Marie, Antonio E Fortunato, Michiel Matthijs, Benjamin Schellenberger Costa, Rudy Vanderhaeghen, Hilde Van Den Daele, Matthias Sachse, et al. 2013. “AUREOCHROME1a-mediated Induction of the Diatom-specific Cyclin dsCYC2 Controls the Onset of Cell Division in Diatoms (Phaeodactylum Tricornutum).” Plant Cell 25 (1): 215–228.
APA
Huysman, M., Fortunato, A. E., Matthijs, M., Costa, B. S., Vanderhaeghen, R., Van Den Daele, H., Sachse, M., et al. (2013). AUREOCHROME1a-mediated induction of the diatom-specific cyclin dsCYC2 controls the onset of cell division in diatoms (Phaeodactylum tricornutum). PLANT CELL, 25(1), 215–228.
Vancouver
1.
Huysman M, Fortunato AE, Matthijs M, Costa BS, Vanderhaeghen R, Van Den Daele H, et al. AUREOCHROME1a-mediated induction of the diatom-specific cyclin dsCYC2 controls the onset of cell division in diatoms (Phaeodactylum tricornutum). PLANT CELL. 2013;25(1):215–28.
MLA
Huysman, Marie, Antonio E Fortunato, Michiel Matthijs, et al. “AUREOCHROME1a-mediated Induction of the Diatom-specific Cyclin dsCYC2 Controls the Onset of Cell Division in Diatoms (Phaeodactylum Tricornutum).” PLANT CELL 25.1 (2013): 215–228. Print.
@article{3188021,
  abstract     = {Cell division in photosynthetic organisms is tightly regulated by light. Although the light dependency of the onset of the cell cycle has been well characterized in various phototrophs, little is known about the cellular signaling cascades connecting light perception to cell cycle activation and progression. Here, we demonstrate that diatom-specific cyclin 2 (dsCYC2) in Phaeodactylum tricornutum displays a transcriptional peak within 15 min after light exposure, long before the onset of cell division. The product of dsCYC2 binds to the cyclin-dependent kinase CDKA1 and can complement G1 cyclin-deficient yeast. Consistent with the role of dsCYC2 in controlling a G1-to-S light-dependent cell cycle checkpoint, dsCYC2 silencing decreases the rate of cell division in diatoms exposed to light-dark cycles but not to constant light. Transcriptional induction of dsCYC2 is triggered by blue light in a fluence rate-dependent manner. Consistent with this, dsCYC2 is a transcriptional target of the blue light sensor AUREOCHROME1a, which functions synergistically with the basic leucine zipper (bZIP) transcription factor bZIP10 to induce dsCYC2 transcription. The functional characterization of a cyclin whose transcription is controlled by light and whose activity connects light signaling to cell cycle progression contributes significantly to our understanding of the molecular mechanisms underlying light-dependent cell cycle onset in diatoms.},
  author       = {Huysman, Marie and Fortunato, Antonio E and Matthijs, Michiel and Costa, Benjamin Schellenberger and Vanderhaeghen, Rudy and Van Den Daele, Hilde and Sachse, Matthias and Inz{\'e}, Dirk and Bowler, Chris and Kroth, Peter G and Wilhelm, Christian and Falciatore, Angela and Vyverman, Wim and De Veylder, Lieven},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {PLANT,YEAST,HIGH LIGHT,SACCHAROMYCES-CEREVISIAE,MARINE-PHYTOPLANKTON,GENE-EXPRESSION,PHOTOPROTECTION,PROGRESSION,PHOTOSYNTHESIS,PROTEIN},
  language     = {eng},
  number       = {1},
  pages        = {215--228},
  title        = {AUREOCHROME1a-mediated induction of the diatom-specific cyclin dsCYC2 controls the onset of cell division in diatoms (Phaeodactylum tricornutum)},
  url          = {http://dx.doi.org/10.1105/tpc.112.106377},
  volume       = {25},
  year         = {2013},
}

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