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Genome-wide expression analysis reveals TORC1-dependent and -independent functions of Sch9

Bart Smets, Pepijn De Snijder, Kristof Engelen, Eva Joossens, Ruben Ghillebert, Karin Thevissen, Kathleen Marchal UGent and Joris Winderickx (2008) FEMS YEAST RESEARCH. 8(8). p.1276-1288
abstract
The protein kinase Sch9 is proposed to be a downstream effector of TORC1 that is required for activation of ribosome biogenesis and repression of entry into G(0). However, Sch9 apparently functions antagonistically to TORC1, when considering the induction of several stress defence genes that are normally repressed by TORC1. To further investigate the relationship between Sch9 and TORC1, we compared the rapamycin-induced transcriptional responses in an sch9 Delta mutant and the isogenic wild type. The data indicate that Sch9 is necessary for proper integration of the rapamycin-induced stress signal, i.e. in sch9 Delta cells, typical effects of rapamycin-like repression of ribosomal protein genes and induction of stress response genes are diminished or abolished. Moreover, they reveal for the first time a direct link between Sch9 and nitrogen metabolism. A sch9 Delta mutant has an increased basal activation of targets of the general amino acid control pathway and of the nitrogen discrimination pathway, including the ammonium permease MEP2 and the amino acid permease GAP1. The mutant also shows enhanced expression of the transcription factor Gcn4 required for amino acid biosynthesis. Our data favour a model in which (1) the role of Sch9 in the general stress response switches depending on TORC1 activity and (2) Sch9 and TORC1 have independent and additive effects on genes induced upon nitrogen and amino acid starvation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
rapamycin, Sch9, yeast, stress response, translation, nitrogen sensing, YEAST SACCHAROMYCES-CEREVISIAE, PROTEIN-KINASE-A, CELL-GROWTH CONTROL, GENE-EXPRESSION, TRANSCRIPTION FACTORS, REGULATED TRANSCRIPTION, AMMONIUM TRANSPORTERS, NUTRIENT AVAILABILITY, SIGNALING PATHWAY, STATIONARY-PHASE, TOR
journal title
FEMS YEAST RESEARCH
FEMS Yeast Res.
volume
8
issue
8
pages
1276 - 1288
Web of Science type
Article
Web of Science id
000260537300008
JCR category
MYCOLOGY
JCR impact factor
2.579 (2008)
JCR rank
4/19 (2008)
JCR quartile
1 (2008)
ISSN
1567-1356
DOI
10.1111/j.1567-1364.2008.00432.x
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3187065
handle
http://hdl.handle.net/1854/LU-3187065
date created
2013-04-08 16:30:10
date last changed
2016-12-19 15:40:04
@article{3187065,
  abstract     = {The protein kinase Sch9 is proposed to be a downstream effector of TORC1 that is required for activation of ribosome biogenesis and repression of entry into G(0). However, Sch9 apparently functions antagonistically to TORC1, when considering the induction of several stress defence genes that are normally repressed by TORC1. To further investigate the relationship between Sch9 and TORC1, we compared the rapamycin-induced transcriptional responses in an sch9 Delta mutant and the isogenic wild type. The data indicate that Sch9 is necessary for proper integration of the rapamycin-induced stress signal, i.e. in sch9 Delta cells, typical effects of rapamycin-like repression of ribosomal protein genes and induction of stress response genes are diminished or abolished. Moreover, they reveal for the first time a direct link between Sch9 and nitrogen metabolism. A sch9 Delta mutant has an increased basal activation of targets of the general amino acid control pathway and of the nitrogen discrimination pathway, including the ammonium permease MEP2 and the amino acid permease GAP1. The mutant also shows enhanced expression of the transcription factor Gcn4 required for amino acid biosynthesis. Our data favour a model in which (1) the role of Sch9 in the general stress response switches depending on TORC1 activity and (2) Sch9 and TORC1 have independent and additive effects on genes induced upon nitrogen and amino acid starvation.},
  author       = {Smets, Bart and De Snijder, Pepijn and Engelen, Kristof and Joossens, Eva and Ghillebert, Ruben and Thevissen, Karin and Marchal, Kathleen and Winderickx, Joris},
  issn         = {1567-1356},
  journal      = {FEMS YEAST RESEARCH},
  keyword      = {rapamycin,Sch9,yeast,stress response,translation,nitrogen sensing,YEAST SACCHAROMYCES-CEREVISIAE,PROTEIN-KINASE-A,CELL-GROWTH CONTROL,GENE-EXPRESSION,TRANSCRIPTION FACTORS,REGULATED TRANSCRIPTION,AMMONIUM TRANSPORTERS,NUTRIENT AVAILABILITY,SIGNALING PATHWAY,STATIONARY-PHASE,TOR},
  language     = {eng},
  number       = {8},
  pages        = {1276--1288},
  title        = {Genome-wide expression analysis reveals TORC1-dependent and -independent functions of Sch9},
  url          = {http://dx.doi.org/10.1111/j.1567-1364.2008.00432.x},
  volume       = {8},
  year         = {2008},
}

Chicago
Smets, Bart, Pepijn De Snijder, Kristof Engelen, Eva Joossens, Ruben Ghillebert, Karin Thevissen, Kathleen Marchal, and Joris Winderickx. 2008. “Genome-wide Expression Analysis Reveals TORC1-dependent and -independent Functions of Sch9.” Fems Yeast Research 8 (8): 1276–1288.
APA
Smets, Bart, De Snijder, P., Engelen, K., Joossens, E., Ghillebert, R., Thevissen, K., Marchal, K., et al. (2008). Genome-wide expression analysis reveals TORC1-dependent and -independent functions of Sch9. FEMS YEAST RESEARCH, 8(8), 1276–1288.
Vancouver
1.
Smets B, De Snijder P, Engelen K, Joossens E, Ghillebert R, Thevissen K, et al. Genome-wide expression analysis reveals TORC1-dependent and -independent functions of Sch9. FEMS YEAST RESEARCH. 2008;8(8):1276–88.
MLA
Smets, Bart, Pepijn De Snijder, Kristof Engelen, et al. “Genome-wide Expression Analysis Reveals TORC1-dependent and -independent Functions of Sch9.” FEMS YEAST RESEARCH 8.8 (2008): 1276–1288. Print.