Ghent University Academic Bibliography

Advanced

PKA and Sch9 control a molecular switch important for the proper adaptation to nutrient availability

Johnny Roosen, Kristof Engelen, Kathleen Marchal UGent, Janick Mathys, Gerard Griffioen, Elisabetta Cameroni, Johan M Thevelein, Claudio De Virgilio, Bart De Moor and Joris Winderickx (2005) MOLECULAR MICROBIOLOGY. 55(3). p.862-880
abstract
In the yeast Saccharomyces cerevisiae, PKA and Sch9 exert similar physiological roles in response to nutrient availability. However, their functional redundancy complicates to distinguish properly the target genes for both kinases. In this article, we analysed different phenotypic read-outs. The data unequivocally showed that both kinases act through separate signalling cascades. In addition, genome-wide expression analysis under conditions and with strains in which either PKA and/or Sch9 signalling was specifically affected, demonstrated that both kinases synergistically or oppositely regulate given gene targets. Unlike PKA, which negatively regulates stress-responsive element (STRE)- and post-diauxic shift (PDS)-driven gene expression, Sch9 appears to exert additional positive control on the Rim15-effector Gis1 to regulate PDS-driven gene expression. The data presented are consistent with a cyclic AMP (cAMP)-gating phenomenon recognized in higher eukaryotes consisting of a main gatekeeper, the protein kinase PKA, switching on or off the activities and signals transmitted through primary pathways such as, in case of yeast, the Sch9-controlled signalling route. This mechanism allows fine-tuning various nutritional responses in yeast cells, allowing them to adapt metabolism and growth appropriately.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
YEAST SACCHAROMYCES-CEREVISIAE, DEPENDENT PROTEIN-KINASE, CYCLIC-AMP PHOSPHODIESTERASE, GENE-EXPRESSION, SIGNAL-TRANSDUCTION, CATALYTIC SUBUNITS, STRESS-RESPONSE, TRANSCRIPTIONAL ACTIVATION, MEDIATED TRANSCRIPTION, NEGATIVE REGULATION
journal title
MOLECULAR MICROBIOLOGY
Mol. Microbiol.
volume
55
issue
3
pages
862 - 880
Web of Science type
Article
Web of Science id
000226457800018
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
6.203 (2005)
JCR rank
33/256 (2005)
JCR quartile
1 (2005)
ISSN
0950-382X
DOI
10.1111/j.1365-2958.2004.04429.x
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3187054
handle
http://hdl.handle.net/1854/LU-3187054
date created
2013-04-08 16:30:10
date last changed
2016-12-19 15:45:57
@article{3187054,
  abstract     = {In the yeast Saccharomyces cerevisiae, PKA and Sch9 exert similar physiological roles in response to nutrient availability. However, their functional redundancy complicates to distinguish properly the target genes for both kinases. In this article, we analysed different phenotypic read-outs. The data unequivocally showed that both kinases act through separate signalling cascades. In addition, genome-wide expression analysis under conditions and with strains in which either PKA and/or Sch9 signalling was specifically affected, demonstrated that both kinases synergistically or oppositely regulate given gene targets. Unlike PKA, which negatively regulates stress-responsive element (STRE)- and post-diauxic shift (PDS)-driven gene expression, Sch9 appears to exert additional positive control on the Rim15-effector Gis1 to regulate PDS-driven gene expression. The data presented are consistent with a cyclic AMP (cAMP)-gating phenomenon recognized in higher eukaryotes consisting of a main gatekeeper, the protein kinase PKA, switching on or off the activities and signals transmitted through primary pathways such as, in case of yeast, the Sch9-controlled signalling route. This mechanism allows fine-tuning various nutritional responses in yeast cells, allowing them to adapt metabolism and growth appropriately.},
  author       = {Roosen, Johnny and Engelen, Kristof and Marchal, Kathleen and Mathys, Janick and Griffioen, Gerard and Cameroni, Elisabetta and Thevelein, Johan M and De Virgilio, Claudio and De Moor, Bart and Winderickx, Joris},
  issn         = {0950-382X},
  journal      = {MOLECULAR MICROBIOLOGY},
  keyword      = {YEAST SACCHAROMYCES-CEREVISIAE,DEPENDENT PROTEIN-KINASE,CYCLIC-AMP PHOSPHODIESTERASE,GENE-EXPRESSION,SIGNAL-TRANSDUCTION,CATALYTIC SUBUNITS,STRESS-RESPONSE,TRANSCRIPTIONAL ACTIVATION,MEDIATED TRANSCRIPTION,NEGATIVE REGULATION},
  language     = {eng},
  number       = {3},
  pages        = {862--880},
  title        = {PKA and Sch9 control a molecular switch important for the proper adaptation to nutrient availability},
  url          = {http://dx.doi.org/10.1111/j.1365-2958.2004.04429.x},
  volume       = {55},
  year         = {2005},
}

Chicago
Roosen, Johnny, Kristof Engelen, Kathleen Marchal, Janick Mathys, Gerard Griffioen, Elisabetta Cameroni, Johan M Thevelein, Claudio De Virgilio, Bart De Moor, and Joris Winderickx. 2005. “PKA and Sch9 Control a Molecular Switch Important for the Proper Adaptation to Nutrient Availability.” Molecular Microbiology 55 (3): 862–880.
APA
Roosen, J., Engelen, K., Marchal, K., Mathys, J., Griffioen, G., Cameroni, E., Thevelein, J. M., et al. (2005). PKA and Sch9 control a molecular switch important for the proper adaptation to nutrient availability. MOLECULAR MICROBIOLOGY, 55(3), 862–880.
Vancouver
1.
Roosen J, Engelen K, Marchal K, Mathys J, Griffioen G, Cameroni E, et al. PKA and Sch9 control a molecular switch important for the proper adaptation to nutrient availability. MOLECULAR MICROBIOLOGY. 2005;55(3):862–80.
MLA
Roosen, Johnny, Kristof Engelen, Kathleen Marchal, et al. “PKA and Sch9 Control a Molecular Switch Important for the Proper Adaptation to Nutrient Availability.” MOLECULAR MICROBIOLOGY 55.3 (2005): 862–880. Print.