Advanced search
1 file | 808.45 KB Add to list

Brominated furanones inhibit biofilm formation by Salmonella enterica serovar Typhimurium

Author
Organization
Abstract
Salmonella enterica serovar Typhimurium is a main cause of bacterial food-borne diseases. As Salmonella can form biofilms in which it is better protected against antimicrobial agents on a wide diversity of surfaces, it is of interest to explore ways to inhibit biofilm formation. Brominated furanones, originally extracted from the marine alga Delisea pulchra, are known to interfere with biofilm formation in several pathogens. In this study, we have synthesized a small focused library of brominated furanones and tested their activity against S. enterica serovar Typhimurium biofilm formation. We show that several furanones inhibit Salmonella biofilm formation at non-growth-inhibiting concentrations. The most interesting compounds are (Z)-4-bromo-5-(bromomethylene)3- alkyl-2(5H)-furanones with chain lengths of two to six carbon atoms. A microarray study was performed to analyze the gene expression profiles of Salmonella in the presence of (Z)-4-bromo-5-(bromomethylene)3- ethyl-2(5H)-furanone. The induced genes include genes that are involved in metabolism, stress response, and drug sensitivity. Most of the repressed genes are involved in metabolism, the type III secretion system, and flagellar biosynthesis. Follow-up experiments confirmed that this furanone interferes with the synthesis of flagella by Salmonella. No evidence was found that furanones act on the currently known quorumsensing systems in Salmonella. Interestingly, pretreatment with furanones rendered Salmonella biofilms more susceptible to antibiotic treatment. Conclusively, this work demonstrates that particular brominated furanones have potential in the prevention of biofilm formation by Salmonella serovar Typhimurium.
Keywords
SERRATIA-LIQUEFACIENS MG1, DIFFERENTIAL GENE-EXPRESSION, TO-CELL COMMUNICATION, QUORUM-SENSING SIGNAL, ALGA DELISEA-PULCHRA, VIBRIO-HARVEYI, HALOGENATED FURANONES, ESCHERICHIA-COLI, SWARMING MOTILITY, BACILLUS-SUBTILIS

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 808.45 KB

Citation

Please use this url to cite or link to this publication:

MLA
Janssens, Joost CA, Hans Steenackers, Stijn Robijns, et al. “Brominated Furanones Inhibit Biofilm Formation by Salmonella Enterica Serovar Typhimurium.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 74.21 (2008): 6639–6648. Print.
APA
Janssens, J. C., Steenackers, H., Robijns, S., Gellens, E., Levin, J., Zhao, H., Hermans, K., et al. (2008). Brominated furanones inhibit biofilm formation by Salmonella enterica serovar Typhimurium. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74(21), 6639–6648.
Chicago author-date
Janssens, Joost CA, Hans Steenackers, Stijn Robijns, Edith Gellens, Jeremy Levin, Hui Zhao, Kim Hermans, et al. 2008. “Brominated Furanones Inhibit Biofilm Formation by Salmonella Enterica Serovar Typhimurium.” Applied and Environmental Microbiology 74 (21): 6639–6648.
Chicago author-date (all authors)
Janssens, Joost CA, Hans Steenackers, Stijn Robijns, Edith Gellens, Jeremy Levin, Hui Zhao, Kim Hermans, David De Coster, Tine L Verhoeven, Kathleen Marchal, Jos Vanderleyden, Dirk E De Vos, and Sigrid CJ De Keersmaecker. 2008. “Brominated Furanones Inhibit Biofilm Formation by Salmonella Enterica Serovar Typhimurium.” Applied and Environmental Microbiology 74 (21): 6639–6648.
Vancouver
1.
Janssens JC, Steenackers H, Robijns S, Gellens E, Levin J, Zhao H, et al. Brominated furanones inhibit biofilm formation by Salmonella enterica serovar Typhimurium. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2008;74(21):6639–48.
IEEE
[1]
J. C. Janssens et al., “Brominated furanones inhibit biofilm formation by Salmonella enterica serovar Typhimurium,” APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 74, no. 21, pp. 6639–6648, 2008.
@article{3186904,
  abstract     = {Salmonella enterica serovar Typhimurium is a main cause of bacterial food-borne diseases. As Salmonella can form biofilms in which it is better protected against antimicrobial agents on a wide diversity of surfaces, it is of interest to explore ways to inhibit biofilm formation. Brominated furanones, originally extracted from the marine alga Delisea pulchra, are known to interfere with biofilm formation in several pathogens. In this study, we have synthesized a small focused library of brominated furanones and tested their activity against S. enterica serovar Typhimurium biofilm formation. We show that several furanones inhibit Salmonella biofilm formation at non-growth-inhibiting concentrations. The most interesting compounds are (Z)-4-bromo-5-(bromomethylene)3- alkyl-2(5H)-furanones with chain lengths of two to six carbon atoms. A microarray study was performed to analyze the gene expression profiles of Salmonella in the presence of (Z)-4-bromo-5-(bromomethylene)3- ethyl-2(5H)-furanone. The induced genes include genes that are involved in metabolism, stress response, and drug sensitivity. Most of the repressed genes are involved in metabolism, the type III secretion system, and flagellar biosynthesis. Follow-up experiments confirmed that this furanone interferes with the synthesis of flagella by Salmonella. No evidence was found that furanones act on the currently known quorumsensing systems in Salmonella. Interestingly, pretreatment with furanones rendered Salmonella biofilms more susceptible to antibiotic treatment. Conclusively, this work demonstrates that particular brominated furanones have potential in the prevention of biofilm formation by Salmonella serovar Typhimurium.},
  author       = {Janssens, Joost CA and Steenackers, Hans and Robijns, Stijn and Gellens, Edith and Levin, Jeremy and Zhao, Hui and Hermans, Kim and De Coster, David and Verhoeven, Tine L and Marchal, Kathleen and Vanderleyden, Jos and De Vos, Dirk E and De Keersmaecker, Sigrid CJ},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keywords     = {SERRATIA-LIQUEFACIENS MG1,DIFFERENTIAL GENE-EXPRESSION,TO-CELL COMMUNICATION,QUORUM-SENSING SIGNAL,ALGA DELISEA-PULCHRA,VIBRIO-HARVEYI,HALOGENATED FURANONES,ESCHERICHIA-COLI,SWARMING MOTILITY,BACILLUS-SUBTILIS},
  language     = {eng},
  number       = {21},
  pages        = {6639--6648},
  title        = {Brominated furanones inhibit biofilm formation by Salmonella enterica serovar Typhimurium},
  url          = {http://dx.doi.org/10.1128/AEM.01262-08},
  volume       = {74},
  year         = {2008},
}

Altmetric
View in Altmetric
Web of Science
Times cited: