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Human hepatic progenitor cells express vasoactive intestinal peptide receptor type 2 and receive nerve endings

(2007) LIVER INTERNATIONAL. 27(3). p.323-328
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Abstract
We recently showed that human hepatic progenitor cells (HPCs) express muscarinic acetylcholine (Ach) receptor subtype 3 and that - following liver transplantation - HPC numbers are significantly reduced. To further elaborate on this, we examined whether HPC also express receptors for vasoactive intestinal peptide (VIP), which, besides Ach, also is an important parasympathetic neurotransmitter. VIP expressing nerves are known to be present in the liver. We performed immunohistochemistry for VIP receptor subtypes 1 and 2 (VIPR1 and 2), on sections of normal and diseased human liver (n=17), and double staining for VIPR2 and known HPC markers. We performed RT-PCR for VIPR1 and 2 on total RNA from purified rat HPC. To document the probability of direct interaction, we also performed double immunostaining for nerve markers and HPC markers on human liver sections. VIPR2 immunostaining was clearly positive in HPC and reactive bile ductules on paraffin-embedded and frozen tissue sections. We could not demonstrate VIPR1 protein expression in the liver, with either of two VIPR1 antibodies tested. The presence of VIPR2 mRNA in HPC was confirmed by RT-PCR. Nerve endings were shown to abut on reactive bile ductules. We show here for the first time that HPC express VIPR2 and receive nerve endings. These features, and the fact that HPC numbers are influenced by the presence or absence of the autonomic innervation of the liver, suggest a direct interaction.
Keywords
parasympathetic, innervation, stem cells, HUMAN LIVER, RAT, POLYPEPTIDE, CHOLANGIOCYTES, INNERVATION, SECRETION, GROWTH, LIGHT

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Citation

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Chicago
Cassiman, David, Nicoletta Sinelli, Ilse Bockx, Sara Vander Borght, Bryon Petersen, Rita De Vos, Jos van Pelt, Frederik Nevens, Louis Libbrecht, and Tania Roskams. 2007. “Human Hepatic Progenitor Cells Express Vasoactive Intestinal Peptide Receptor Type 2 and Receive Nerve Endings.” Liver International 27 (3): 323–328.
APA
Cassiman, D., Sinelli, N., Bockx, I., Vander Borght, S., Petersen, B., De Vos, R., van Pelt, J., et al. (2007). Human hepatic progenitor cells express vasoactive intestinal peptide receptor type 2 and receive nerve endings. LIVER INTERNATIONAL, 27(3), 323–328.
Vancouver
1.
Cassiman D, Sinelli N, Bockx I, Vander Borght S, Petersen B, De Vos R, et al. Human hepatic progenitor cells express vasoactive intestinal peptide receptor type 2 and receive nerve endings. LIVER INTERNATIONAL. 2007;27(3):323–8.
MLA
Cassiman, David, Nicoletta Sinelli, Ilse Bockx, et al. “Human Hepatic Progenitor Cells Express Vasoactive Intestinal Peptide Receptor Type 2 and Receive Nerve Endings.” LIVER INTERNATIONAL 27.3 (2007): 323–328. Print.
@article{3183641,
  abstract     = {We recently showed that human hepatic progenitor cells (HPCs) express muscarinic acetylcholine (Ach) receptor subtype 3 and that - following liver transplantation - HPC numbers are significantly reduced. To further elaborate on this, we examined whether HPC also express receptors for vasoactive intestinal peptide (VIP), which, besides Ach, also is an important parasympathetic neurotransmitter. VIP expressing nerves are known to be present in the liver. 
We performed immunohistochemistry for VIP receptor subtypes 1 and 2 (VIPR1 and 2), on sections of normal and diseased human liver (n=17), and double staining for VIPR2 and known HPC markers. We performed RT-PCR for VIPR1 and 2 on total RNA from purified rat HPC. To document the probability of direct interaction, we also performed double immunostaining for nerve markers and HPC markers on human liver sections. 
VIPR2 immunostaining was clearly positive in HPC and reactive bile ductules on paraffin-embedded and frozen tissue sections. We could not demonstrate VIPR1 protein expression in the liver, with either of two VIPR1 antibodies tested. The presence of VIPR2 mRNA in HPC was confirmed by RT-PCR. Nerve endings were shown to abut on reactive bile ductules. 
We show here for the first time that HPC express VIPR2 and receive nerve endings. These features, and the fact that HPC numbers are influenced by the presence or absence of the autonomic innervation of the liver, suggest a direct interaction.},
  author       = {Cassiman, David and Sinelli, Nicoletta and Bockx, Ilse and Vander Borght, Sara and Petersen, Bryon and De Vos, Rita and van Pelt, Jos and Nevens, Frederik and Libbrecht, Louis and Roskams, Tania},
  issn         = {1478-3223},
  journal      = {LIVER INTERNATIONAL},
  keywords     = {parasympathetic,innervation,stem cells,HUMAN LIVER,RAT,POLYPEPTIDE,CHOLANGIOCYTES,INNERVATION,SECRETION,GROWTH,LIGHT},
  language     = {eng},
  number       = {3},
  pages        = {323--328},
  title        = {Human hepatic progenitor cells express vasoactive intestinal peptide receptor type 2 and receive nerve endings},
  url          = {http://dx.doi.org/10.1111/j.1478-3231.2006.01427.x},
  volume       = {27},
  year         = {2007},
}

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