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Breast cancer resistance protein (BCRP/ABCG2) is expressed by progenitor cells/reactive ductules and hepatocytes and its expression pattern is influenced by disease etiology and species type: possible functional consequences

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Abstract
Breast cancer resistance protein (BCRP/ABCG2) is an ATP-binding cassette transport protein that is expressed in several organs including the liver. Previous studies have shown that ABC transport proteins play an important pathophysiological role in several liver diseases. However, to date, expression pattern and possible role of BCRP in human liver diseases and animal models have not been studied in detail. Here we investigated the expression pattern of BCRP in normal liver, chronic parenchymal and biliary human liver diseases, and parallel in different rat models of liver diseases. Expression was studied by immunohistochemistry and additionally by RT-PCR analysis in Thy-l-positive rat oval cells. Bile ducts, hepatic progenitor cells, reactive bile ductules, and blood vessel endothelium were immunoreactive for BCRP in normal liver and all types of human liver diseases and in rat models. BCRP was expressed by the canalicular membrane of hepatocytes in normal and diseased human liver, but never in rat liver. Remarkably, there was also expression of BCRP at the basolateral pole of human hepatocytes, and this was most pronounced in chronic biliary diseases. In conclusion, BCRP positivity in the progenitor cells/reactive ductules could contribute to the resistance of these cells to cytotoxic agents and xenotoxins. Basolateral hepatocytic expression in chronic biliary diseases may be an adaptive mechanism to pump bile constituents back into the sinusoidal blood. Strong differences between human and rat liver must be taken into account in future studies with animal models.
Keywords
immunohistochemistry, breast cancer resistance protein, basolateral expression, hepatic progenitor cells, multidrug resistance phenotype, human and rat liver, HEPATIC OVAL CELLS, PRIMARY BILIARY-CIRRHOSIS, DIPEPTIDYL-PEPTIDASE-IV, HUMAN LIVER-DISEASE, MARROW STEM-CELLS, RAT-LIVER, HEPATOCELLULAR TRANSPORTERS, POPULATION, LOCALIZATION, ABCG2

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MLA
Vander Borght, Sara, Louis Libbrecht, Aezarn Katoonizadeh, et al. “Breast Cancer Resistance Protein (BCRP/ABCG2) Is Expressed by Progenitor Cells/reactive Ductules and Hepatocytes and Its Expression Pattern Is Influenced by Disease Etiology and Species Type: Possible Functional Consequences.” JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 54.9 (2006): 1051–1059. Print.
APA
Vander Borght, S., Libbrecht, L., Katoonizadeh, A., van Pelt, J., Cassiman, D., Nevens, F., Van Lommel, A., et al. (2006). Breast cancer resistance protein (BCRP/ABCG2) is expressed by progenitor cells/reactive ductules and hepatocytes and its expression pattern is influenced by disease etiology and species type: possible functional consequences. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 54(9), 1051–1059.
Chicago author-date
Vander Borght, Sara, Louis Libbrecht, Aezarn Katoonizadeh, Jos van Pelt, David Cassiman, Frederik Nevens, Alfons Van Lommel, et al. 2006. “Breast Cancer Resistance Protein (BCRP/ABCG2) Is Expressed by Progenitor Cells/reactive Ductules and Hepatocytes and Its Expression Pattern Is Influenced by Disease Etiology and Species Type: Possible Functional Consequences.” Journal of Histochemistry & Cytochemistry 54 (9): 1051–1059.
Chicago author-date (all authors)
Vander Borght, Sara, Louis Libbrecht, Aezarn Katoonizadeh, Jos van Pelt, David Cassiman, Frederik Nevens, Alfons Van Lommel, Bryon E Petersen, Johan Fevery, Peter L Jansen, and Tania A Roskams. 2006. “Breast Cancer Resistance Protein (BCRP/ABCG2) Is Expressed by Progenitor Cells/reactive Ductules and Hepatocytes and Its Expression Pattern Is Influenced by Disease Etiology and Species Type: Possible Functional Consequences.” Journal of Histochemistry & Cytochemistry 54 (9): 1051–1059.
Vancouver
1.
Vander Borght S, Libbrecht L, Katoonizadeh A, van Pelt J, Cassiman D, Nevens F, et al. Breast cancer resistance protein (BCRP/ABCG2) is expressed by progenitor cells/reactive ductules and hepatocytes and its expression pattern is influenced by disease etiology and species type: possible functional consequences. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. 2006;54(9):1051–9.
IEEE
[1]
S. Vander Borght et al., “Breast cancer resistance protein (BCRP/ABCG2) is expressed by progenitor cells/reactive ductules and hepatocytes and its expression pattern is influenced by disease etiology and species type: possible functional consequences,” JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, vol. 54, no. 9, pp. 1051–1059, 2006.
@article{3183536,
  abstract     = {Breast cancer resistance protein (BCRP/ABCG2) is an ATP-binding cassette transport protein that is expressed in several organs including the liver. Previous studies have shown that ABC transport proteins play an important pathophysiological role in several liver diseases. However, to date, expression pattern and possible role of BCRP in human liver diseases and animal models have not been studied in detail. Here we investigated the expression pattern of BCRP in normal liver, chronic parenchymal and biliary human liver diseases, and parallel in different rat models of liver diseases. Expression was studied by immunohistochemistry and additionally by RT-PCR analysis in Thy-l-positive rat oval cells. Bile ducts, hepatic progenitor cells, reactive bile ductules, and blood vessel endothelium were immunoreactive for BCRP in normal liver and all types of human liver diseases and in rat models. BCRP was expressed by the canalicular membrane of hepatocytes in normal and diseased human liver, but never in rat liver. Remarkably, there was also expression of BCRP at the basolateral pole of human hepatocytes, and this was most pronounced in chronic biliary diseases. In conclusion, BCRP positivity in the progenitor cells/reactive ductules could contribute to the resistance of these cells to cytotoxic agents and xenotoxins. Basolateral hepatocytic expression in chronic biliary diseases may be an adaptive mechanism to pump bile constituents back into the sinusoidal blood. Strong differences between human and rat liver must be taken into account in future studies with animal models.},
  author       = {Vander Borght, Sara and Libbrecht, Louis and Katoonizadeh, Aezarn and van Pelt, Jos and Cassiman, David and Nevens, Frederik and Van Lommel, Alfons and Petersen, Bryon E and Fevery, Johan and Jansen, Peter L and Roskams, Tania A},
  issn         = {0022-1554},
  journal      = {JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY},
  keywords     = {immunohistochemistry,breast cancer resistance protein,basolateral expression,hepatic progenitor cells,multidrug resistance phenotype,human and rat liver,HEPATIC OVAL CELLS,PRIMARY BILIARY-CIRRHOSIS,DIPEPTIDYL-PEPTIDASE-IV,HUMAN LIVER-DISEASE,MARROW STEM-CELLS,RAT-LIVER,HEPATOCELLULAR TRANSPORTERS,POPULATION,LOCALIZATION,ABCG2},
  language     = {eng},
  number       = {9},
  pages        = {1051--1059},
  title        = {Breast cancer resistance protein (BCRP/ABCG2) is expressed by progenitor cells/reactive ductules and hepatocytes and its expression pattern is influenced by disease etiology and species type: possible functional consequences},
  url          = {http://dx.doi.org/10.1369/jhc.5A6912.2006},
  volume       = {54},
  year         = {2006},
}

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