Advanced search
1 file | 2.26 MB Add to list

The vagal nerve stimulates activation of the hepatic progenitor cell compartment via muscarinic acetylcholine receptor type 3

(2002) AMERICAN JOURNAL OF PATHOLOGY. 161(2). p.521-530
Author
Organization
Abstract
In the rat the hepatic branch of the nervus vagus stimulates proliferation of hepatocytes after partial hepatectomy and growth of bile duct epithelial cells after bile duct ligation. We studied the effect of hepatic vagotomy on the activation of the hepatic progenitor cell compartment in human and rat liver. The number of hepatic progenitor cells and atypical reactive ductular cells in transplanted (denervated) human livers with hepatitis was significantly lower than in innervated matched control livers and the number of oval cells in vagotomized rat livers with galactosamine hepatitis was significantly lower than in livers of sham-operated rats with galactosamine hepatitis. The expression of muscarinic acetylcholine receptors (M1-M5 receptor) was studied by immunohistochemistry and reverse transcriptase-polymerase chain reaction. In human fiver, immunoreactivity for M3 receptor was observed in hepatic progenitor cells, atypical reactive ductules, intermediate hepatocyte-like cells, and bile duct epithelial cells. mRNA for the M1-M3 and the M5 receptor, but not the M4 receptor, was detected in human liver homogenates. in conclusion, the hepatic vagus branch stimulates activation of the hepatic progenitor cell compartment in diseased liver, most likely through binding of acetylcholine to the M3 receptor expressed on these cells. These findings may be of clinical importance for patients with a transplant liver.
Keywords
OVAL CELLS, CHRONIC VIRAL-HEPATITIS, RAT-LIVER, BONE-MARROW, STEM-CELLS, HYPOTHALAMIC-LESIONS, PARTIAL-HEPATECTOMY, DUCTULAR REACTION, STELLATE CELLS, GROWTH-FACTOR

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 2.26 MB

Citation

Please use this url to cite or link to this publication:

MLA
Cassiman, David, Louis Libbrecht, Nicoletta Sinelli, et al. “The Vagal Nerve Stimulates Activation of the Hepatic Progenitor Cell Compartment via Muscarinic Acetylcholine Receptor Type 3.” AMERICAN JOURNAL OF PATHOLOGY 161.2 (2002): 521–530. Print.
APA
Cassiman, D., Libbrecht, L., Sinelli, N., Desmet, V., Denef, C., & Roskams, T. (2002). The vagal nerve stimulates activation of the hepatic progenitor cell compartment via muscarinic acetylcholine receptor type 3. AMERICAN JOURNAL OF PATHOLOGY, 161(2), 521–530.
Chicago author-date
Cassiman, David, Louis Libbrecht, Nicoletta Sinelli, Valeer Desmet, Carl Denef, and Tania Roskams. 2002. “The Vagal Nerve Stimulates Activation of the Hepatic Progenitor Cell Compartment via Muscarinic Acetylcholine Receptor Type 3.” American Journal of Pathology 161 (2): 521–530.
Chicago author-date (all authors)
Cassiman, David, Louis Libbrecht, Nicoletta Sinelli, Valeer Desmet, Carl Denef, and Tania Roskams. 2002. “The Vagal Nerve Stimulates Activation of the Hepatic Progenitor Cell Compartment via Muscarinic Acetylcholine Receptor Type 3.” American Journal of Pathology 161 (2): 521–530.
Vancouver
1.
Cassiman D, Libbrecht L, Sinelli N, Desmet V, Denef C, Roskams T. The vagal nerve stimulates activation of the hepatic progenitor cell compartment via muscarinic acetylcholine receptor type 3. AMERICAN JOURNAL OF PATHOLOGY. 2002;161(2):521–30.
IEEE
[1]
D. Cassiman, L. Libbrecht, N. Sinelli, V. Desmet, C. Denef, and T. Roskams, “The vagal nerve stimulates activation of the hepatic progenitor cell compartment via muscarinic acetylcholine receptor type 3,” AMERICAN JOURNAL OF PATHOLOGY, vol. 161, no. 2, pp. 521–530, 2002.
@article{3183221,
  abstract     = {In the rat the hepatic branch of the nervus vagus stimulates proliferation of hepatocytes after partial hepatectomy and growth of bile duct epithelial cells after bile duct ligation. We studied the effect of hepatic vagotomy on the activation of the hepatic progenitor cell compartment in human and rat liver. The number of hepatic progenitor cells and atypical reactive ductular cells in transplanted (denervated) human livers with hepatitis was significantly lower than in innervated matched control livers and the number of oval cells in vagotomized rat livers with galactosamine hepatitis was significantly lower than in livers of sham-operated rats with galactosamine hepatitis. The expression of muscarinic acetylcholine receptors (M1-M5 receptor) was studied by immunohistochemistry and reverse transcriptase-polymerase chain reaction. In human fiver, immunoreactivity for M3 receptor was observed in hepatic progenitor cells, atypical reactive ductules, intermediate hepatocyte-like cells, and bile duct epithelial cells. mRNA for the M1-M3 and the M5 receptor, but not the M4 receptor, was detected in human liver homogenates. in conclusion, the hepatic vagus branch stimulates activation of the hepatic progenitor cell compartment in diseased liver, most likely through binding of acetylcholine to the M3 receptor expressed on these cells. These findings may be of clinical importance for patients with a transplant liver.},
  author       = {Cassiman, David and Libbrecht, Louis and Sinelli, Nicoletta and Desmet, Valeer and Denef, Carl and Roskams, Tania},
  issn         = {0002-9440},
  journal      = {AMERICAN JOURNAL OF PATHOLOGY},
  keywords     = {OVAL CELLS,CHRONIC VIRAL-HEPATITIS,RAT-LIVER,BONE-MARROW,STEM-CELLS,HYPOTHALAMIC-LESIONS,PARTIAL-HEPATECTOMY,DUCTULAR REACTION,STELLATE CELLS,GROWTH-FACTOR},
  language     = {eng},
  number       = {2},
  pages        = {521--530},
  title        = {The vagal nerve stimulates activation of the hepatic progenitor cell compartment via muscarinic acetylcholine receptor type 3},
  url          = {http://dx.doi.org/10.1016/S0002-9440(10)64208-3},
  volume       = {161},
  year         = {2002},
}

Altmetric
View in Altmetric
Web of Science
Times cited: