- Author
- Pawel Tulinski, Ad C Fluit, Jos PM van Putten, Alain de Bruin, Sarah Glorieux (UGent) , Jaap A Wagenaar and Birgitta Duim
- Organization
- Abstract
- Staphylococcus aureus is an opportunistic pathogen able to colonize the upper respiratory tract and skin surfaces in mammals. Methicillin-resistant S. aureus ST398 is prevalent in pigs in Europe and North America. However, the mechanism of successful pig colonization by MRSA ST398 is poorly understood. To study MRSA colonization in pigs, an ex vivo model consisting of porcine nasal mucosa explants cultured at an air-liquid interface was evaluated. In cultured mucosa explants from the surfaces of the ventral turbinates and septum of the pig nose no changes in cell morphology and viability were observed up to 72 h. MRSA colonization on the explants was evaluated followed for three MRSA ST398 isolates for 180 minutes. The explants were incubated with 3x10(8) CFU/ml in PBS for 2 h to allow bacteria to adhere to the explants surface. Next the explants were washed and in the first 30 minutes post adhering time, a decline in the number of CFU was observed for all MRSA. Subsequently, the isolates showed either: bacterial growth, no growth, or a further reduction in bacterial numbers. The MRSA were either localized as clusters between the cilia or as single bacteria on the cilia surface. No morphological changes in the epithelium layer were observed during the incubation with MRSA. We conclude that porcine nasal mucosa explants are a valuable ex vivo model to unravel the interaction of MRSA with nasal tissue.
- Keywords
- BACTERIAL, CARRIAGE, INTERFACE, ADHERENCE, METHICILLIN-RESISTANT, RESISTANT STAPHYLOCOCCUS-AUREUS, CULTURE, HUMANS, PIGS, MICE
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-3168187
- MLA
- Tulinski, Pawel, et al. “An Ex Vivo Porcine Nasal Mucosa Explants Model to Study MRSA Colonization.” PLOS ONE, vol. 8, no. 1, 2013, doi:10.1371/journal.pone.0053783.
- APA
- Tulinski, P., Fluit, A. C., van Putten, J. P., de Bruin, A., Glorieux, S., Wagenaar, J. A., & Duim, B. (2013). An ex vivo porcine nasal mucosa explants model to study MRSA colonization. PLOS ONE, 8(1). https://doi.org/10.1371/journal.pone.0053783
- Chicago author-date
- Tulinski, Pawel, Ad C Fluit, Jos PM van Putten, Alain de Bruin, Sarah Glorieux, Jaap A Wagenaar, and Birgitta Duim. 2013. “An Ex Vivo Porcine Nasal Mucosa Explants Model to Study MRSA Colonization.” PLOS ONE 8 (1). https://doi.org/10.1371/journal.pone.0053783.
- Chicago author-date (all authors)
- Tulinski, Pawel, Ad C Fluit, Jos PM van Putten, Alain de Bruin, Sarah Glorieux, Jaap A Wagenaar, and Birgitta Duim. 2013. “An Ex Vivo Porcine Nasal Mucosa Explants Model to Study MRSA Colonization.” PLOS ONE 8 (1). doi:10.1371/journal.pone.0053783.
- Vancouver
- 1.Tulinski P, Fluit AC, van Putten JP, de Bruin A, Glorieux S, Wagenaar JA, et al. An ex vivo porcine nasal mucosa explants model to study MRSA colonization. PLOS ONE. 2013;8(1).
- IEEE
- [1]P. Tulinski et al., “An ex vivo porcine nasal mucosa explants model to study MRSA colonization,” PLOS ONE, vol. 8, no. 1, 2013.
@article{3168187, abstract = {{Staphylococcus aureus is an opportunistic pathogen able to colonize the upper respiratory tract and skin surfaces in mammals. Methicillin-resistant S. aureus ST398 is prevalent in pigs in Europe and North America. However, the mechanism of successful pig colonization by MRSA ST398 is poorly understood. To study MRSA colonization in pigs, an ex vivo model consisting of porcine nasal mucosa explants cultured at an air-liquid interface was evaluated. In cultured mucosa explants from the surfaces of the ventral turbinates and septum of the pig nose no changes in cell morphology and viability were observed up to 72 h. MRSA colonization on the explants was evaluated followed for three MRSA ST398 isolates for 180 minutes. The explants were incubated with 3x10(8) CFU/ml in PBS for 2 h to allow bacteria to adhere to the explants surface. Next the explants were washed and in the first 30 minutes post adhering time, a decline in the number of CFU was observed for all MRSA. Subsequently, the isolates showed either: bacterial growth, no growth, or a further reduction in bacterial numbers. The MRSA were either localized as clusters between the cilia or as single bacteria on the cilia surface. No morphological changes in the epithelium layer were observed during the incubation with MRSA. We conclude that porcine nasal mucosa explants are a valuable ex vivo model to unravel the interaction of MRSA with nasal tissue.}}, articleno = {{e53783}}, author = {{Tulinski, Pawel and Fluit, Ad C and van Putten, Jos PM and de Bruin, Alain and Glorieux, Sarah and Wagenaar, Jaap A and Duim, Birgitta}}, issn = {{1932-6203}}, journal = {{PLOS ONE}}, keywords = {{BACTERIAL,CARRIAGE,INTERFACE,ADHERENCE,METHICILLIN-RESISTANT,RESISTANT STAPHYLOCOCCUS-AUREUS,CULTURE,HUMANS,PIGS,MICE}}, language = {{eng}}, number = {{1}}, pages = {{7}}, title = {{An ex vivo porcine nasal mucosa explants model to study MRSA colonization}}, url = {{http://doi.org/10.1371/journal.pone.0053783}}, volume = {{8}}, year = {{2013}}, }
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