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Inducible system for lateral roots in Arabidopsis thaliana and maize

Leentje Jansen (UGent) , Boris Parizot (UGent) and Tom Beeckman (UGent)
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Abstract
The study of biological processes contributing to plant growth can be complicated by the small number of cells involved and by the brief and sudden appearance of some crucial developmental steps. Given such troublesome circumstances, methods to monitor the timing or to increase the number of concerned cells can be of great advantage to researchers. Lateral root initiation is a process taking place endogenously in a discrete number of cells of the parent root. It represents the onset of the formation of a new meristem and provides the below ground part of the plant the ability to react on environmental conditions such as nutrient and water availability. Insights into the underlying mechanisms of this developmental event can be of major importance to provide means of improving tolerance to nutrient and water de fi cient conditions. The exact timing and site of lateral root initiation are, however, impossible to predict, hampering exhaustive studies of the sequence of events directing this process. Here, we present a method to synchronize the induction of lateral roots in Arabidopsis thaliana and maize. By initially preventing the formation of laterals in young seedlings and subsequently inducing lateral root initiation, this method not only allows controlling the process in time but also enlarges significantly the population of cells involved, opening up the way to systems biology approaches.
Keywords
Lateral roots, Maize, Arabidopsis thaliana, Auxin, Paper rolls

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Chicago
Jansen, Leentje, Boris Parizot, and Tom Beeckman. 2013. “Inducible System for Lateral Roots in Arabidopsis Thaliana and Maize.” In Plant Organogenesis : Methods and Protocols, ed. Ive De Smet, 959:149–158. New York, NY, USA: Humana Press.
APA
Jansen, L., Parizot, B., & Beeckman, T. (2013). Inducible system for lateral roots in Arabidopsis thaliana and maize. In Ive De Smet (Ed.), Plant organogenesis : methods and protocols (Vol. 959, pp. 149–158). New York, NY, USA: Humana Press.
Vancouver
1.
Jansen L, Parizot B, Beeckman T. Inducible system for lateral roots in Arabidopsis thaliana and maize. In: De Smet I, editor. Plant organogenesis : methods and protocols. New York, NY, USA: Humana Press; 2013. p. 149–58.
MLA
Jansen, Leentje, Boris Parizot, and Tom Beeckman. “Inducible System for Lateral Roots in Arabidopsis Thaliana and Maize.” Plant Organogenesis : Methods and Protocols. Ed. Ive De Smet. Vol. 959. New York, NY, USA: Humana Press, 2013. 149–158. Print.
@incollection{3165935,
  abstract     = {The study of biological processes contributing to plant growth can be complicated by the small number of cells involved and by the brief and sudden appearance of some crucial developmental steps. Given such troublesome circumstances, methods to monitor the timing or to increase the number of concerned cells can be of great advantage to researchers. Lateral root initiation is a process taking place endogenously in a discrete number of cells of the parent root. It represents the onset of the formation of a new meristem and provides the below ground part of the plant the ability to react on environmental conditions such as nutrient and water availability. Insights into the underlying mechanisms of this developmental event can be of major importance to provide means of improving tolerance to nutrient and water de fi cient conditions. The exact timing and site of lateral root initiation are, however, impossible to predict, hampering exhaustive studies of the sequence of events directing this process. Here, we present a method to synchronize the induction of lateral roots in Arabidopsis thaliana and maize. By initially preventing the formation of laterals in young seedlings and subsequently inducing lateral root initiation, this method not only allows controlling the process in time but also enlarges significantly the population of cells involved, opening up the way to systems biology approaches.},
  author       = {Jansen, Leentje and Parizot, Boris and Beeckman, Tom},
  booktitle    = {Plant organogenesis : methods and protocols},
  editor       = {De Smet, Ive},
  isbn         = {9781627032216},
  issn         = {1064-3745},
  keyword      = {Lateral roots,Maize,Arabidopsis thaliana,Auxin,Paper rolls},
  language     = {eng},
  pages        = {149--158},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Inducible system for lateral roots in Arabidopsis thaliana and maize},
  url          = {http://dx.doi.org/10.1007/978-1-62703-221-6\_9},
  volume       = {959},
  year         = {2013},
}

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