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PYRABACTIN RESISTANCE1-LIKE8 plays an important role for the regulation of abscisic acid signaling in root

(2013) PLANT PHYSIOLOGY. 161(2). p.931-941
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Abstract
Abscisic acid (ABA) signaling plays a critical role in regulating root growth and root system architecture. ABA-mediated growth promotion and root tropic response under water stress are key responses for plant survival under limiting water conditions. In this work, we have explored the role of Arabidopsis (Arabidopsis thaliana) PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS for root ABA signaling. As a result, we discovered that PYL8 plays a nonredundant role for the regulation of root ABA sensitivity. Unexpectedly, given the multigenic nature and partial functional redundancy observed in the PYR/PYL family, the single pyl8 mutant showed reduced sensitivity to ABA-mediated root growth inhibition. This effect was due to the lack of PYL8-mediated inhibition of several clade A phosphatases type 2C (PP2Cs), since PYL8 interacted in vivo with at least five PP2Cs, namely HYPERSENSITIVE TO ABA1 (HAB1), HAB2, ABA-INSENSITIVE1 (ABI1), ABI2, and PP2CA/ABA-HYPERSENSITIVE GERMINATION3 as revealed by tandem affinity purification and mass spectrometry proteomic approaches. We also discovered that PYR/PYL receptors and clade A PP2Cs are crucial for the hydrotropic response that takes place to guide root growth far from regions with low water potential. Thus, an ABA-hypersensitive pp2c quadruple mutant showed enhanced hydrotropism, whereas an ABA-insensitive sextuple pyr/pyl mutant showed reduced hydrotropic response, indicating that ABA-dependent inhibition of PP2Cs by PYR/PYLs is required for the proper perception of a moisture gradient.
Keywords
DIFFERENT PHOSPHORYLATION MECHANISMS, TANDEM AFFINITY PURIFICATION, PHOSPHATASES TYPE 2C, HYDROTROPISM, GENE-EXPRESSION, ARABIDOPSIS-THALIANA, PROTEIN PHOSPHATASES, KINASES, GROWTH MAINTENANCE, GRAVITROPISM

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MLA
Antoni, Regina, Miguel Gonzalez-Guzman, Lesia Rodriguez, et al. “PYRABACTIN RESISTANCE1-LIKE8 Plays an Important Role for the Regulation of Abscisic Acid Signaling in Root.” PLANT PHYSIOLOGY 161.2 (2013): 931–941. Print.
APA
Antoni, R., Gonzalez-Guzman, M., Rodriguez, L., Peirats-Llobet, M., Pizzio, G. A., Fernandez, M. A., De Winne, N., et al. (2013). PYRABACTIN RESISTANCE1-LIKE8 plays an important role for the regulation of abscisic acid signaling in root. PLANT PHYSIOLOGY, 161(2), 931–941.
Chicago author-date
Antoni, Regina, Miguel Gonzalez-Guzman, Lesia Rodriguez, Marta Peirats-Llobet, Gaston A Pizzio, Maria A Fernandez, Nancy De Winne, et al. 2013. “PYRABACTIN RESISTANCE1-LIKE8 Plays an Important Role for the Regulation of Abscisic Acid Signaling in Root.” Plant Physiology 161 (2): 931–941.
Chicago author-date (all authors)
Antoni, Regina, Miguel Gonzalez-Guzman, Lesia Rodriguez, Marta Peirats-Llobet, Gaston A Pizzio, Maria A Fernandez, Nancy De Winne, Geert De Jaeger, Daniela Dietrich, Malcom J Bennett, and Pedro L Rodriguez. 2013. “PYRABACTIN RESISTANCE1-LIKE8 Plays an Important Role for the Regulation of Abscisic Acid Signaling in Root.” Plant Physiology 161 (2): 931–941.
Vancouver
1.
Antoni R, Gonzalez-Guzman M, Rodriguez L, Peirats-Llobet M, Pizzio GA, Fernandez MA, et al. PYRABACTIN RESISTANCE1-LIKE8 plays an important role for the regulation of abscisic acid signaling in root. PLANT PHYSIOLOGY. 2013;161(2):931–41.
IEEE
[1]
R. Antoni et al., “PYRABACTIN RESISTANCE1-LIKE8 plays an important role for the regulation of abscisic acid signaling in root,” PLANT PHYSIOLOGY, vol. 161, no. 2, pp. 931–941, 2013.
@article{3165853,
  abstract     = {Abscisic acid (ABA) signaling plays a critical role in regulating root growth and root system architecture. ABA-mediated growth promotion and root tropic response under water stress are key responses for plant survival under limiting water conditions. In this work, we have explored the role of Arabidopsis (Arabidopsis thaliana) PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS for root ABA signaling. As a result, we discovered that PYL8 plays a nonredundant role for the regulation of root ABA sensitivity. Unexpectedly, given the multigenic nature and partial functional redundancy observed in the PYR/PYL family, the single pyl8 mutant showed reduced sensitivity to ABA-mediated root growth inhibition. This effect was due to the lack of PYL8-mediated inhibition of several clade A phosphatases type 2C (PP2Cs), since PYL8 interacted in vivo with at least five PP2Cs, namely HYPERSENSITIVE TO ABA1 (HAB1), HAB2, ABA-INSENSITIVE1 (ABI1), ABI2, and PP2CA/ABA-HYPERSENSITIVE GERMINATION3 as revealed by tandem affinity purification and mass spectrometry proteomic approaches. We also discovered that PYR/PYL receptors and clade A PP2Cs are crucial for the hydrotropic response that takes place to guide root growth far from regions with low water potential. Thus, an ABA-hypersensitive pp2c quadruple mutant showed enhanced hydrotropism, whereas an ABA-insensitive sextuple pyr/pyl mutant showed reduced hydrotropic response, indicating that ABA-dependent inhibition of PP2Cs by PYR/PYLs is required for the proper perception of a moisture gradient.},
  author       = {Antoni, Regina and Gonzalez-Guzman, Miguel and Rodriguez, Lesia and Peirats-Llobet, Marta and Pizzio, Gaston A and Fernandez, Maria A and De Winne, Nancy and De Jaeger, Geert and Dietrich, Daniela and Bennett, Malcom J and Rodriguez, Pedro L},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keywords     = {DIFFERENT PHOSPHORYLATION MECHANISMS,TANDEM AFFINITY PURIFICATION,PHOSPHATASES TYPE 2C,HYDROTROPISM,GENE-EXPRESSION,ARABIDOPSIS-THALIANA,PROTEIN PHOSPHATASES,KINASES,GROWTH MAINTENANCE,GRAVITROPISM},
  language     = {eng},
  number       = {2},
  pages        = {931--941},
  title        = {PYRABACTIN RESISTANCE1-LIKE8 plays an important role for the regulation of abscisic acid signaling in root},
  url          = {http://dx.doi.org/10.1104/pp.112.208678},
  volume       = {161},
  year         = {2013},
}

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