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Microbiotas from UC patients display altered metabolism and reduced ability of LAB to colonize mucus

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Abstract
We compared fecal microbial communities derived either from Ulcerative Colitis (UC) patients in remission (n=4) or in relapse (n=4), or from healthy subjects (n=4). These communities were used for inoculation of a dynamic in vitro gut model, which contained integrated mucin-covered microcosms. We found that the microbiota of the 'mucus' largely differed from that of the 'lumen'. This was partly due to decreased mucus-associated populations of lactic acid producing bacterial populations (LAB), as LAB originating from UC patients had a significantly decreased capacity to colonize the mucin-covered microcosms as compared to those originating from healthy subjects. We found significant differences between the metabolomes of UC patients in relapse and remission, respectively, while the metabolome of patients in remission resembled that of healthy subjects. These novel findings constitute an important contribution to the understanding of the complex etiology of UC.
Keywords
HUMAN INTESTINAL MUCUS, INFLAMMATORY-BOWEL-DISEASE, DYNAMIC GUT MODEL, ULCERATIVE-COLITIS, FECAL MICROBIOTA, RECTAL MUCOSA, INDOLIC COMPOUNDS, ACID BACTERIA, LACTOBACILLUS, COMMUNITY

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Chicago
Vigsnaes, Louise Kristine, Pieter Van den Abbeele, Karolina Sulek, Henrik Lauritz Frandsen, Casper Steenholdt, Jørn Brynskov, Joan Vermeiren, Tom Van de Wiele, and Tine Rask Licht. 2013. “Microbiotas from UC Patients Display Altered Metabolism and Reduced Ability of LAB to Colonize Mucus.” Scientific Reports 3.
APA
Vigsnaes, L. K., Van den Abbeele, P., Sulek, K., Frandsen, H. L., Steenholdt, C., Brynskov, J., Vermeiren, J., et al. (2013). Microbiotas from UC patients display altered metabolism and reduced ability of LAB to colonize mucus. SCIENTIFIC REPORTS, 3.
Vancouver
1.
Vigsnaes LK, Van den Abbeele P, Sulek K, Frandsen HL, Steenholdt C, Brynskov J, et al. Microbiotas from UC patients display altered metabolism and reduced ability of LAB to colonize mucus. SCIENTIFIC REPORTS. 2013;3.
MLA
Vigsnaes, Louise Kristine et al. “Microbiotas from UC Patients Display Altered Metabolism and Reduced Ability of LAB to Colonize Mucus.” SCIENTIFIC REPORTS 3 (2013): n. pag. Print.
@article{3165238,
  abstract     = {We compared fecal microbial communities derived either from Ulcerative Colitis (UC) patients in remission (n=4) or in relapse (n=4), or from healthy subjects (n=4). These communities were used for inoculation of a dynamic in vitro gut model, which contained integrated mucin-covered microcosms. We found that the microbiota of the 'mucus' largely differed from that of the 'lumen'. This was partly due to decreased mucus-associated populations of lactic acid producing bacterial populations (LAB), as LAB originating from UC patients had a significantly decreased capacity to colonize the mucin-covered microcosms as compared to those originating from healthy subjects. We found significant differences between the metabolomes of UC patients in relapse and remission, respectively, while the metabolome of patients in remission resembled that of healthy subjects. These novel findings constitute an important contribution to the understanding of the complex etiology of UC.},
  articleno    = {1110},
  author       = {Vigsnaes, Louise Kristine and Van den Abbeele, Pieter and Sulek, Karolina and Frandsen, Henrik Lauritz and Steenholdt, Casper and Brynskov, Jørn and Vermeiren, Joan and Van de Wiele, Tom and Licht, Tine Rask},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keywords     = {HUMAN INTESTINAL MUCUS,INFLAMMATORY-BOWEL-DISEASE,DYNAMIC GUT MODEL,ULCERATIVE-COLITIS,FECAL MICROBIOTA,RECTAL MUCOSA,INDOLIC COMPOUNDS,ACID BACTERIA,LACTOBACILLUS,COMMUNITY},
  language     = {eng},
  pages        = {10},
  title        = {Microbiotas from UC patients display altered metabolism and reduced ability of LAB to colonize mucus},
  url          = {http://dx.doi.org/10.1038/srep01110},
  volume       = {3},
  year         = {2013},
}

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