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Prebiotic approach alleviates hepatic steatosis: implication of fatty acid oxidative and cholesterol synthesis pathways

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Abstract
Scope : Recent data suggest that gut microbiota contributes to the regulation of host lipid metabolism. We report how fermentable dietary fructo-oligosaccharides (FOS) control hepatic steatosis induced by n-3 PUFA depletion, which leads to hepatic alterations similar to those observed in non-alcoholic fatty liver disease patients. Methods and results : C57Bl/6J mice fed an n-3 PUFA-depleted diet for 3 months were supplemented with FOS during the last 10 days of treatment. FOS-treated mice exhibited higher caecal Bifidobacterium spp. and lower Roseburia spp. content. Microarray analysis of hepatic mRNA revealed that FOS supplementation reduced hepatic triglyceride accumulation through a proliferator-activated receptor a-stimulation of fatty acid oxidation and lessened cholesterol accumulation by inhibiting sterol regulatory element binding protein 2-dependent cholesterol synthesis. Cultured precision-cut liver slices confirmed the inhibition of fatty acid oxidation. FOS effects were related to a decreased hepatic micro-RNA33 expression and to an increased colonic glucagon-like peptide 1 production. Conclusions : The changes in gut microbiota composition by n-3 PUFA-depletion and prebiotics modulate hepatic steatosis by changing gene expression in the liver, a phenomenon that could implicate micro-RNA and gut-derived hormones. Our data underline the advantage of targeting the gut microbiota by colonic nutrients in the management of liver disease.
Keywords
micro-RNA 33, Gut microbiota, n-3-PUFA, Peroxisome proliferator-activated receptor, Sterol regulatory element binding protein, CONJUGATED LINOLEIC-ACID, GLUCAGON-LIKE PEPTIDE-1, INULIN-TYPE FRUCTANS, ZUCKER FA/FA RATS, LIVER-DISEASE, GUT MICROBIOTA, LIPID-METABOLISM, DIETARY FRUCTANS, GENE-EXPRESSION, PPAR-ALPHA

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Citation

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Chicago
Pachikian, Barbara D, Ahmed Essaghir, Jean-Baptitste Demoulin, Emilie Catry, Aaudrey M Neyrinck, Evelyne M Dewulf, Florence M Sohet, et al. 2013. “Prebiotic Approach Alleviates Hepatic Steatosis: Implication of Fatty Acid Oxidative and Cholesterol Synthesis Pathways.” Molecular Nutrition & Food Research 57 (2): 347–359.
APA
Pachikian, B. D., Essaghir, A., Demoulin, J.-B., Catry, E., Neyrinck, A. M., Dewulf, E. M., Sohet, F. M., et al. (2013). Prebiotic approach alleviates hepatic steatosis: implication of fatty acid oxidative and cholesterol synthesis pathways. MOLECULAR NUTRITION & FOOD RESEARCH, 57(2), 347–359.
Vancouver
1.
Pachikian BD, Essaghir A, Demoulin J-B, Catry E, Neyrinck AM, Dewulf EM, et al. Prebiotic approach alleviates hepatic steatosis: implication of fatty acid oxidative and cholesterol synthesis pathways. MOLECULAR NUTRITION & FOOD RESEARCH. 2013;57(2):347–59.
MLA
Pachikian, Barbara D, Ahmed Essaghir, Jean-Baptitste Demoulin, et al. “Prebiotic Approach Alleviates Hepatic Steatosis: Implication of Fatty Acid Oxidative and Cholesterol Synthesis Pathways.” MOLECULAR NUTRITION & FOOD RESEARCH 57.2 (2013): 347–359. Print.
@article{3165195,
  abstract     = {Scope : Recent data suggest that gut microbiota contributes to the regulation of host lipid metabolism. We report how fermentable dietary fructo-oligosaccharides (FOS) control hepatic steatosis induced by n-3 PUFA depletion, which leads to hepatic alterations similar to those observed in non-alcoholic fatty liver disease patients.
Methods and results : C57Bl/6J mice fed an n-3 PUFA-depleted diet for 3 months were supplemented with FOS during the last 10 days of treatment. FOS-treated mice exhibited higher caecal Bifidobacterium spp. and lower Roseburia spp. content. Microarray analysis of hepatic mRNA revealed that FOS supplementation reduced hepatic triglyceride accumulation through a proliferator-activated receptor a-stimulation of fatty acid oxidation and lessened cholesterol accumulation by inhibiting sterol regulatory element binding protein 2-dependent cholesterol synthesis. Cultured precision-cut liver slices confirmed the inhibition of fatty acid oxidation. FOS effects were related to a decreased hepatic micro-RNA33 expression and to an increased colonic glucagon-like peptide 1 production.
Conclusions : The changes in gut microbiota composition by n-3 PUFA-depletion and prebiotics modulate hepatic steatosis by changing gene expression in the liver, a phenomenon that could implicate micro-RNA and gut-derived hormones. Our data underline the advantage of targeting the gut microbiota by colonic nutrients in the management of liver disease.},
  author       = {Pachikian, Barbara D and Essaghir, Ahmed and Demoulin, Jean-Baptitste and Catry, Emilie and Neyrinck, Aaudrey M and Dewulf, Evelyne M and Sohet, Florence M and Portois, Laurence and Clerbaux, Laure-Alix and Carpentier, Yvon A and Possemiers, Sam and Bommer, Guido T and Cani, Patrice D and Delzenne, Nathalie M},
  issn         = {1613-4125},
  journal      = {MOLECULAR NUTRITION \& FOOD RESEARCH},
  language     = {eng},
  number       = {2},
  pages        = {347--359},
  title        = {Prebiotic approach alleviates hepatic steatosis: implication of fatty acid oxidative and cholesterol synthesis pathways},
  url          = {http://dx.doi.org/10.1002/mnfr.201200364},
  volume       = {57},
  year         = {2013},
}

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