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Selective inhibition of Cx43 hemichannels by Gap19 and its impact on myocardial ischemia/reperfusion injury

Nan Wang UGent, Elke De Vuyst UGent, Raf Ponsaerts, Kerstin Boengler, Nicolás Palacios-Prado, Joris Wauman UGent, Charles P Lai, Marijke De Bock UGent, Elke Decrock UGent, Mélissa Bol UGent, et al. (2013) BASIC RESEARCH IN CARDIOLOGY. 108(1).
abstract
Connexin-43 (Cx43), a predominant cardiac connexin, forms gap junctions (GJs) that facilitate electrical cell-cell coupling and unapposed/nonjunctional hemichannels that provide a pathway for the exchange of ions and metabolites between cytoplasm and extracellular milieu. Uncontrolled opening of hemichannels in the plasma membrane may be deleterious for the myocardium and blocking hemichannels may confer cardioprotection by preventing ionic imbalance, cell swelling and loss of critical metabolites. Currently, all known hemichannel inhibitors also block GJ channels, thereby disturbing electrical cell-cell communication. Here we aimed to characterize a nonapeptide, called Gap19, derived from the cytoplasmic loop (CL) of Cx43 as a hemichannel blocker and examined its effect on hemichannel currents in cardiomyocytes and its influence in cardiac outcome after ischemia/reperfusion. We report that Gap 19 inhibits Cx43 hemichannels without blocking GJ channels or Cx40/pannexin-1 hemichannels. Hemichannel inhibition is due to the binding of Gap19 to the C-terminus (CT) thereby preventing intramolecular CT-CL interactions. The peptide inhibited Cx43 hemichannel unitary currents in both HeLa cells exogenously expressing Cx43 and acutely isolated pig ventricular cardiomyocytes. Treatment with Gap19 prevented metabolic inhibition-enhanced hemichannel openings, protected cardiomyocytes against volume overload and cell death following ischemia/reperfusion in vitro and modestly decreased the infarct size after myocardial ischemia/reperfusion in mice in vivo. We conclude that preventing Cx43 hemichannel opening with Gap19 confers limited protective effects against myocardial ischemia/reperfusion injury.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CARDIOMYOCYTE MITOCHONDRIA, MEMBRANE PERMEABILIZATION, INTERCELLULAR COMMUNICATION, OCULODENTODIGITAL DYSPLASIA, JUNCTION CHANNELS, METABOLIC INHIBITION, CONNEXIN MIMETIC PEPTIDES, Myocardial injury, Single channel, Gap junction, Hemichannel, Connexin, ISCHEMIA-REPERFUSION, CARDIAC MYOCYTES, CYTOPLASMIC LOOP
journal title
BASIC RESEARCH IN CARDIOLOGY
Basic Res. Cardiol.
volume
108
issue
1
article number
309
pages
16 pages
Web of Science type
Article
Web of Science id
000314641700001
JCR category
CARDIAC & CARDIOVASCULAR SYSTEMS
JCR impact factor
5.955 (2013)
JCR rank
13/125 (2013)
JCR quartile
1 (2013)
ISSN
0300-8428
DOI
10.1007/s00395-012-0309-x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3162333
handle
http://hdl.handle.net/1854/LU-3162333
date created
2013-03-11 14:35:13
date last changed
2016-12-21 15:41:27
@article{3162333,
  abstract     = {Connexin-43 (Cx43), a predominant cardiac connexin, forms gap junctions (GJs) that facilitate electrical cell-cell coupling and unapposed/nonjunctional hemichannels that provide a pathway for the exchange of ions and metabolites between cytoplasm and extracellular milieu. Uncontrolled opening of hemichannels in the plasma membrane may be deleterious for the myocardium and blocking hemichannels may confer cardioprotection by preventing ionic imbalance, cell swelling and loss of critical metabolites. Currently, all known hemichannel inhibitors also block GJ channels, thereby disturbing electrical cell-cell communication. Here we aimed to characterize a nonapeptide, called Gap19, derived from the cytoplasmic loop (CL) of Cx43 as a hemichannel blocker and examined its effect on hemichannel currents in cardiomyocytes and its influence in cardiac outcome after ischemia/reperfusion. We report that Gap 19 inhibits Cx43 hemichannels without blocking GJ channels or Cx40/pannexin-1 hemichannels. Hemichannel inhibition is due to the binding of Gap19 to the C-terminus (CT) thereby preventing intramolecular CT-CL interactions. The peptide inhibited Cx43 hemichannel unitary currents in both HeLa cells exogenously expressing Cx43 and acutely isolated pig ventricular cardiomyocytes. Treatment with Gap19 prevented metabolic inhibition-enhanced hemichannel openings, protected cardiomyocytes against volume overload and cell death following ischemia/reperfusion in vitro and modestly decreased the infarct size after myocardial ischemia/reperfusion in mice in vivo. We conclude that preventing Cx43 hemichannel opening with Gap19 confers limited protective effects against myocardial ischemia/reperfusion injury.},
  articleno    = {309},
  author       = {Wang, Nan and De Vuyst, Elke and Ponsaerts, Raf and Boengler, Kerstin and Palacios-Prado, Nicol{\'a}s and Wauman, Joris and Lai, Charles P and De Bock, Marijke and Decrock, Elke and Bol, M{\'e}lissa and Vinken, Mathieu and Rogiers, Vera and Tavernier, Jan and Evans, W Howard and Naus, Christian C and Bukauskas, Feliksas F and Sipido, Karin R and Heusch, Gerd and Schulz, Rainer and Bultynck, Geert and Leybaert, Luc},
  issn         = {0300-8428},
  journal      = {BASIC RESEARCH IN CARDIOLOGY},
  keyword      = {CARDIOMYOCYTE MITOCHONDRIA,MEMBRANE PERMEABILIZATION,INTERCELLULAR COMMUNICATION,OCULODENTODIGITAL DYSPLASIA,JUNCTION CHANNELS,METABOLIC INHIBITION,CONNEXIN MIMETIC PEPTIDES,Myocardial injury,Single channel,Gap junction,Hemichannel,Connexin,ISCHEMIA-REPERFUSION,CARDIAC MYOCYTES,CYTOPLASMIC LOOP},
  language     = {eng},
  number       = {1},
  pages        = {16},
  title        = {Selective inhibition of Cx43 hemichannels by Gap19 and its impact on myocardial ischemia/reperfusion injury},
  url          = {http://dx.doi.org/10.1007/s00395-012-0309-x},
  volume       = {108},
  year         = {2013},
}

Chicago
Wang, Nan, Elke De Vuyst, Raf Ponsaerts, Kerstin Boengler, Nicolás Palacios-Prado, Joris Wauman, Charles P Lai, et al. 2013. “Selective Inhibition of Cx43 Hemichannels by Gap19 and Its Impact on Myocardial Ischemia/reperfusion Injury.” Basic Research in Cardiology 108 (1).
APA
Wang, N., De Vuyst, E., Ponsaerts, R., Boengler, K., Palacios-Prado, N., Wauman, J., Lai, C. P., et al. (2013). Selective inhibition of Cx43 hemichannels by Gap19 and its impact on myocardial ischemia/reperfusion injury. BASIC RESEARCH IN CARDIOLOGY, 108(1).
Vancouver
1.
Wang N, De Vuyst E, Ponsaerts R, Boengler K, Palacios-Prado N, Wauman J, et al. Selective inhibition of Cx43 hemichannels by Gap19 and its impact on myocardial ischemia/reperfusion injury. BASIC RESEARCH IN CARDIOLOGY. 2013;108(1).
MLA
Wang, Nan, Elke De Vuyst, Raf Ponsaerts, et al. “Selective Inhibition of Cx43 Hemichannels by Gap19 and Its Impact on Myocardial Ischemia/reperfusion Injury.” BASIC RESEARCH IN CARDIOLOGY 108.1 (2013): n. pag. Print.