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Evaluation of metaphylactic RNA interference to prevent equine herpesvirus type 1 infection in experimental herpesvirus myeloencephalopathy in horses

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Abstract
Objective-To evaluate metaphylactic RNA interference to prevent equine herpesvirus type 1 (EHV-1) infection in experimental herpesvirus myeloencephalopathy in horses and to determine whether horses infected with a neuropathogenic strain of the virus that develop equine herpesvirus myeloencephalopathy (EHM) have differences in viremia. Animals-13 seronegative horses. Procedures-EHV-1 strain Ab4 was administered intranasally on day 0, and small interfering RNAs (siRNAs [EHV-1 specific siRNAs {n = 7} or an irrelevant siRNA {6}]) were administered intranasally 24 hours before and 12, 24, 36, and 48 hours after infection. Physical and neurologic examinations, nasal swab specimens, and blood samples were collected for virus isolation and quantitative PCR assay. Data from the study were combined with data from a previous study of 14 horses. Results-No significant difference was detected in clinical variables, viremia, or detection of EHV-1 in nasal swab specimens of horses treated with the EHV-1 targeted siRNAs (sigB(3)-siOri(2)) versus controls. No significant differences in viremia were detected between horses that developed EHM and those that did not. Conclusions and Clinical Relevance-Administration of siRNAs targeted against EHV-1 around the time of EHV-1 infection was not protective with this experimental design. Horses infected with the neuropathogenic EHV-1 strain Ab4 that developed EHM did not have a more pronounced viremia.
Keywords
NASAL MUCOSAL EXPLANTS, SIRNA, NEUROLOGIC DISEASE, DNA-POLYMERASE, ADULT HORSES, EHV-1, PHARMACOKINETICS, ACYCLOVIR, VIRUS, VACCINES

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Chicago
Perkins, Gillian A, Gerlinde Van de Walle, Nicola Pusterla, Hollis N Erb, and Nikolaus Osterrieder. 2013. “Evaluation of Metaphylactic RNA Interference to Prevent Equine Herpesvirus Type 1 Infection in Experimental Herpesvirus Myeloencephalopathy in Horses.” American Journal of Veterinary Research 74 (2): 248–256.
APA
Perkins, G. A., Van de Walle, G., Pusterla, N., Erb, H. N., & Osterrieder, N. (2013). Evaluation of metaphylactic RNA interference to prevent equine herpesvirus type 1 infection in experimental herpesvirus myeloencephalopathy in horses. AMERICAN JOURNAL OF VETERINARY RESEARCH, 74(2), 248–256.
Vancouver
1.
Perkins GA, Van de Walle G, Pusterla N, Erb HN, Osterrieder N. Evaluation of metaphylactic RNA interference to prevent equine herpesvirus type 1 infection in experimental herpesvirus myeloencephalopathy in horses. AMERICAN JOURNAL OF VETERINARY RESEARCH. 2013;74(2):248–56.
MLA
Perkins, Gillian A, Gerlinde Van de Walle, Nicola Pusterla, et al. “Evaluation of Metaphylactic RNA Interference to Prevent Equine Herpesvirus Type 1 Infection in Experimental Herpesvirus Myeloencephalopathy in Horses.” AMERICAN JOURNAL OF VETERINARY RESEARCH 74.2 (2013): 248–256. Print.
@article{3153012,
  abstract     = {Objective-To evaluate metaphylactic RNA interference to prevent equine herpesvirus type 1 (EHV-1) infection in experimental herpesvirus myeloencephalopathy in horses and to determine whether horses infected with a neuropathogenic strain of the virus that develop equine herpesvirus myeloencephalopathy (EHM) have differences in viremia. 
Animals-13 seronegative horses. 
Procedures-EHV-1 strain Ab4 was administered intranasally on day 0, and small interfering RNAs (siRNAs [EHV-1 specific siRNAs \{n = 7\} or an irrelevant siRNA \{6\}]) were administered intranasally 24 hours before and 12, 24, 36, and 48 hours after infection. Physical and neurologic examinations, nasal swab specimens, and blood samples were collected for virus isolation and quantitative PCR assay. Data from the study were combined with data from a previous study of 14 horses. 
Results-No significant difference was detected in clinical variables, viremia, or detection of EHV-1 in nasal swab specimens of horses treated with the EHV-1 targeted siRNAs (sigB(3)-siOri(2)) versus controls. No significant differences in viremia were detected between horses that developed EHM and those that did not. 
Conclusions and Clinical Relevance-Administration of siRNAs targeted against EHV-1 around the time of EHV-1 infection was not protective with this experimental design. Horses infected with the neuropathogenic EHV-1 strain Ab4 that developed EHM did not have a more pronounced viremia.},
  author       = {Perkins, Gillian A and Van de Walle, Gerlinde and Pusterla, Nicola and Erb, Hollis N and Osterrieder, Nikolaus},
  issn         = {0002-9645},
  journal      = {AMERICAN JOURNAL OF VETERINARY RESEARCH},
  keyword      = {NASAL MUCOSAL EXPLANTS,SIRNA,NEUROLOGIC DISEASE,DNA-POLYMERASE,ADULT HORSES,EHV-1,PHARMACOKINETICS,ACYCLOVIR,VIRUS,VACCINES},
  language     = {eng},
  number       = {2},
  pages        = {248--256},
  title        = {Evaluation of metaphylactic RNA interference to prevent equine herpesvirus type 1 infection in experimental herpesvirus myeloencephalopathy in horses},
  url          = {http://dx.doi.org/10.2460/ajvr.74.2.248},
  volume       = {74},
  year         = {2013},
}

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