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Effect of organic acids on Salmonella colonization and shedding in weaned piglets in a seeder model

Joris Michiels UGent, Joris Missotten, Geertrui Rasschaert, Noël Dierick UGent, Marc Heyndrickx UGent and Stefaan De Smet UGent (2012) JOURNAL OF FOOD PROTECTION. 75(11). p.1974-1983
abstract
Piglets (n = 128) weaned at 21 days of age were used in a 35-day seeder model to evaluate the effects of dietary additives differing in active ingredients, chemical, and physical formulation, and dose on Salmonella colonization and shedding and intestinal microbial populations. Treatments were a negative control (basal diet), the positive control (challenged, basal diet), and six treatments similar to the positive control but supplemented with the following active ingredients (dose excluding essential oils or natural extracts): triglycerides with butyric acid (1.30 g kg(-1)); formic and citric acids and essential oils (2.44 g kg(-1)); coated formic, coated sorbic, and benzoic acids (2.70 g kg(-1)); salts of formic, sorbic, acetic, and propionic acids, their free acids, and natural extracts (2.92 g kg(-1)); triglycerides with caproic and caprylic acids and coated oregano oil (1.80 g kg(-1)); and caproic, caprylic, lauric, and lactic acids (1.91 g kg(-1)). On day 6, half the piglets (seeder pigs) in each group were orally challenged with a Salmonella Typhimurium nalidixic acid-resistant strain (4 x 10(9) and 1.2 x 10(9) log CFU per pig in replicate experiments 1 and 2, respectively). Two days later, they were transferred to pens with an equal number of contact pigs. Salmonella shedding was determined 2 days after challenge exposure and then on a weekly basis. On day 34 or 35, piglets were euthanized to sample tonsils, ileocecal lymph nodes, and ileal and cecal digesta contents. The two additives, both containing short-chain fatty acids and one of them also containing benzoic acid and the other one also containing essential oils, and supplemented at more than 2.70 g kg(-1), showed evidence of reducing Salmonella fecal shedding and numbers of coliforms and Salmonella in cecal digesta. However, colonization of tonsils and ileocecal lymph nodes by Salmonella was not affected. Supplementing butyric acid and medium-chain fatty acids at the applied dose failed to inhibit Salmonella contamination in the current experimental setup.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
EXOGENOUS LIPOLYTIC ENZYMES, CHAIN FATTY-ACIDS, ENTERICA SEROVAR TYPHIMURIUM, GROWTH-PERFORMANCE, IN-VITRO, NUTRITIONAL ANTIBIOTICS, LACTIC-ACID, GUT FLORA, PIGS, FEED
journal title
JOURNAL OF FOOD PROTECTION
J. Food Prot.
volume
75
issue
11
pages
1974 - 1983
Web of Science type
Article
Web of Science id
000311014200008
JCR category
FOOD SCIENCE & TECHNOLOGY
JCR impact factor
1.832 (2012)
JCR rank
44/123 (2012)
JCR quartile
2 (2012)
ISSN
0362-028X
DOI
10.4315/0362-028X.JFP-12-210
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3148169
handle
http://hdl.handle.net/1854/LU-3148169
date created
2013-02-27 10:40:33
date last changed
2016-12-19 15:45:31
@article{3148169,
  abstract     = {Piglets (n = 128) weaned at 21 days of age were used in a 35-day seeder model to evaluate the effects of dietary additives differing in active ingredients, chemical, and physical formulation, and dose on Salmonella colonization and shedding and intestinal microbial populations. Treatments were a negative control (basal diet), the positive control (challenged, basal diet), and six treatments similar to the positive control but supplemented with the following active ingredients (dose excluding essential oils or natural extracts): triglycerides with butyric acid (1.30 g kg(-1)); formic and citric acids and essential oils (2.44 g kg(-1)); coated formic, coated sorbic, and benzoic acids (2.70 g kg(-1)); salts of formic, sorbic, acetic, and propionic acids, their free acids, and natural extracts (2.92 g kg(-1)); triglycerides with caproic and caprylic acids and coated oregano oil (1.80 g kg(-1)); and caproic, caprylic, lauric, and lactic acids (1.91 g kg(-1)). On day 6, half the piglets (seeder pigs) in each group were orally challenged with a Salmonella Typhimurium nalidixic acid-resistant strain (4 x 10(9) and 1.2 x 10(9) log CFU per pig in replicate experiments 1 and 2, respectively). Two days later, they were transferred to pens with an equal number of contact pigs. Salmonella shedding was determined 2 days after challenge exposure and then on a weekly basis. On day 34 or 35, piglets were euthanized to sample tonsils, ileocecal lymph nodes, and ileal and cecal digesta contents. The two additives, both containing short-chain fatty acids and one of them also containing benzoic acid and the other one also containing essential oils, and supplemented at more than 2.70 g kg(-1), showed evidence of reducing Salmonella fecal shedding and numbers of coliforms and Salmonella in cecal digesta. However, colonization of tonsils and ileocecal lymph nodes by Salmonella was not affected. Supplementing butyric acid and medium-chain fatty acids at the applied dose failed to inhibit Salmonella contamination in the current experimental setup.},
  author       = {Michiels, Joris and Missotten, Joris and Rasschaert, Geertrui and Dierick, No{\"e}l and Heyndrickx, Marc and De Smet, Stefaan},
  issn         = {0362-028X},
  journal      = {JOURNAL OF FOOD PROTECTION},
  keyword      = {EXOGENOUS LIPOLYTIC ENZYMES,CHAIN FATTY-ACIDS,ENTERICA SEROVAR TYPHIMURIUM,GROWTH-PERFORMANCE,IN-VITRO,NUTRITIONAL ANTIBIOTICS,LACTIC-ACID,GUT FLORA,PIGS,FEED},
  language     = {eng},
  number       = {11},
  pages        = {1974--1983},
  title        = {Effect of organic acids on Salmonella colonization and shedding in weaned piglets in a seeder model},
  url          = {http://dx.doi.org/10.4315/0362-028X.JFP-12-210},
  volume       = {75},
  year         = {2012},
}

Chicago
Michiels, Joris, Joris Missotten, Geertrui Rasschaert, Noël Dierick, Marc Heyndrickx, and Stefaan De Smet. 2012. “Effect of Organic Acids on Salmonella Colonization and Shedding in Weaned Piglets in a Seeder Model.” Journal of Food Protection 75 (11): 1974–1983.
APA
Michiels, J., Missotten, J., Rasschaert, G., Dierick, N., Heyndrickx, M., & De Smet, S. (2012). Effect of organic acids on Salmonella colonization and shedding in weaned piglets in a seeder model. JOURNAL OF FOOD PROTECTION, 75(11), 1974–1983.
Vancouver
1.
Michiels J, Missotten J, Rasschaert G, Dierick N, Heyndrickx M, De Smet S. Effect of organic acids on Salmonella colonization and shedding in weaned piglets in a seeder model. JOURNAL OF FOOD PROTECTION. 2012;75(11):1974–83.
MLA
Michiels, Joris, Joris Missotten, Geertrui Rasschaert, et al. “Effect of Organic Acids on Salmonella Colonization and Shedding in Weaned Piglets in a Seeder Model.” JOURNAL OF FOOD PROTECTION 75.11 (2012): 1974–1983. Print.