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Transmission of primary resistance mutation K103N in a cluster of Belgian young patients from different risk groups

J Ruelle, M Ingels, K Jnaoui, N Ausselet, A Sasse, Chris Verhofstede UGent and P Goubau (2012) JOURNAL OF THE INTERNATIONAL AIDS SOCIETY. 15(suppl. 4).
abstract
Background: We analysed the distribution of an HIV-1 subtype B strain resistant to efavirenz and nevirapine among incident infections in the Belgian population. Method: The Belgian AIDS reference laboratories searched their databases for HIV-1 subtype B sequences harbouring the K103N mutation in the reverse transcriptase (RT) or the C67S and V77I mutations in the protease (PR). We included the earliest RT sequence available of drug-naïve patients as well as sequences related to treatment failure. Fifty sequences were aligned omitting the codon 103 and submitted to phylogenetic analysis. Epidemiological data were collected through the Institute of Public Health national database. In addition, three sequences from the cluster were analysed by deep sequencing using the Roche GS Junior platform. Results: Phylogenetic analysis revealed the presence of a 24 virus sequences cluster. All except one of those sequences resulted from patients who were ARV-naïve at the time of sampling, and 21 had the K103N mutation. Two thirds of the clustered patients were infected through homosexual or bisexual contacts while the others were heterosexuals. No case was related to migrants contaminated abroad. Fifteen of the clustered patients were diagnosed between January 2011 and June 2012; 87% of them were aged between 20 and 29 at the time of diagnosis. Interestingly, 60% of them reside in the province of Namur. Deep sequencing analysis of 3 individuals sampled near seroconversion revealed no other resistance mutations at a frequency > 1% than those already picked up by Sanger sequencing (RT A98S, K103N; PR V77I), except the RT V90I. Conclusion: We identified a transmission cluster of drug resistant HIV-1 variants mainly including homo- and heterosexual young adults. Most individuals are of Belgian origin and are living around the city of Namur (Belgium). The K103N mutation had no apparent impact on transmission fitness as its spread raised during the last years. These observations may impact on local prevention and ARV prophylaxis strategies.
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author
organization
year
type
conference (meetingAbstract)
publication status
published
subject
in
JOURNAL OF THE INTERNATIONAL AIDS SOCIETY
J. Int. AIDS Soc.
volume
15
issue
suppl. 4
article number
18201
conference name
11th International congress on Drugs Therapy in HIV Infection
conference location
Glasgow, Scotland, UK
conference start
2012-11-11
conference end
2012-11-15
JCR category
INFECTIOUS DISEASES
JCR impact factor
3.936 (2012)
JCR rank
17/69 (2012)
JCR quartile
1 (2012)
ISSN
1758-2652
DOI
10.7448/IAS.15.6.18201
language
English
UGent publication?
yes
classification
C3
copyright statement
I have retained and own the full copyright for this publication
id
3141450
handle
http://hdl.handle.net/1854/LU-3141450
date created
2013-02-25 10:17:08
date last changed
2017-10-26 14:29:39
@inproceedings{3141450,
  abstract     = {Background: We analysed the distribution of an HIV-1 subtype B strain resistant to efavirenz and nevirapine among incident infections in the Belgian population.
Method: The Belgian AIDS reference laboratories searched their databases for HIV-1 subtype B sequences harbouring the K103N mutation in the reverse transcriptase (RT) or the C67S and V77I mutations in the protease (PR). We included the earliest RT sequence available of drug-na{\"i}ve patients as well as sequences related to treatment failure. Fifty sequences were aligned omitting the codon 103 and submitted to phylogenetic analysis. Epidemiological data were collected through the Institute of Public Health national database. In addition, three sequences from the cluster were analysed by deep sequencing using the Roche GS Junior platform.
Results: Phylogenetic analysis revealed the presence of a 24 virus sequences cluster. All except one of those sequences resulted from patients who were ARV-na{\"i}ve at the time of sampling, and 21 had the K103N mutation. Two thirds of the clustered patients were infected through homosexual or bisexual contacts while the others were heterosexuals. No case was related to migrants contaminated abroad. Fifteen of the clustered patients were diagnosed between January 2011 and June 2012; 87\% of them were aged between 20 and 29 at the time of diagnosis. Interestingly, 60\% of them reside in the province of Namur. Deep sequencing analysis of 3 individuals sampled near seroconversion revealed no other resistance mutations at a frequency {\textrangle} 1\% than those already picked up by Sanger sequencing (RT A98S, K103N; PR V77I), except the RT V90I.
Conclusion: We identified a transmission cluster of drug resistant HIV-1 variants mainly including homo- and heterosexual young adults. Most individuals are of Belgian origin and are living around the city of Namur (Belgium). The K103N mutation had no apparent impact on transmission fitness as its spread raised during the last years. These observations may impact on local prevention and ARV prophylaxis strategies.},
  articleno    = {18201},
  author       = {Ruelle, J and Ingels, M and Jnaoui, K and Ausselet, N and Sasse, A and Verhofstede, Chris and Goubau, P},
  booktitle    = {JOURNAL OF THE INTERNATIONAL AIDS SOCIETY},
  issn         = {1758-2652},
  language     = {eng},
  location     = {Glasgow, Scotland, UK},
  number       = {suppl. 4},
  title        = {Transmission of primary resistance mutation K103N in a cluster of Belgian young patients from different risk groups},
  url          = {http://dx.doi.org/10.7448/IAS.15.6.18201},
  volume       = {15},
  year         = {2012},
}

Chicago
Ruelle, J, M Ingels, K Jnaoui, N Ausselet, A Sasse, Chris Verhofstede, and P Goubau. 2012. “Transmission of Primary Resistance Mutation K103N in a Cluster of Belgian Young Patients from Different Risk Groups.” In Journal of the International Aids Society. Vol. 15.
APA
Ruelle, J, Ingels, M., Jnaoui, K., Ausselet, N., Sasse, A., Verhofstede, C., & Goubau, P. (2012). Transmission of primary resistance mutation K103N in a cluster of Belgian young patients from different risk groups. JOURNAL OF THE INTERNATIONAL AIDS SOCIETY (Vol. 15). Presented at the 11th International congress on Drugs Therapy in HIV Infection.
Vancouver
1.
Ruelle J, Ingels M, Jnaoui K, Ausselet N, Sasse A, Verhofstede C, et al. Transmission of primary resistance mutation K103N in a cluster of Belgian young patients from different risk groups. JOURNAL OF THE INTERNATIONAL AIDS SOCIETY. 2012.
MLA
Ruelle, J, M Ingels, K Jnaoui, et al. “Transmission of Primary Resistance Mutation K103N in a Cluster of Belgian Young Patients from Different Risk Groups.” Journal of the International Aids Society. Vol. 15. 2012. Print.