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Isolation and characterisation of an antifolate insensitive (afi1) mutant of Arabidopsis thaliana

Oscar Navarrete UGent, Jeroen Van Daele, Christophe Stove UGent, Willy Lambert, Serguei Storojenko UGent and Dominique Van Der Straeten UGent (2013) PLANT BIOLOGY. 15(1). p.37-44
abstract
Antifolates can impair the synthesis and/or function of folates in living organisms. Mechanisms of resistance or tolerance to antifolates have been mainly described in plants using the drug methotrexate. In this work, the antifolate trimethoprim (TMP) was used with the aim of revealing a novel mechanism of resistance. EMS mutagenised seeds from Arabidopsis were screened to isolate individuals insensitive to TMP. Genetic analysis revealed a homozygous recessive mutation that segregates with the phenotype of tolerance to 50 mu m TMP. Mapping analysis localised the mutation at the end of the short arm of chromosome 3. Preliminary characterisation demonstrated up-regulation of several genes from the folate biosynthetic pathway in the TMP insensitive mutant, and a slight increase in total folate content in the mutant as compared with the Col-0 control. Moreover, sequence analysis of the DHFR (dihydrofolate reductase) genes, which encode a known target for resistance to antifolates, did not reveal any changes. This study is the first report of a stable mutant insensitive (afi1) to the antifolate trimethoprim in plants, and suggests the existence of a novel mechanism of resistance to antifolates.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
mapping, trimethoprim, folate biosynthesis, EMS mutant, Arabidopsis thaliana, Antifolate, RESISTANT CELL-LINES, REDUCTASE THYMIDYLATE SYNTHASE, DIHYDROFOLATE-REDUCTASE, METHOTREXATE-RESISTANCE, FOLATE ANALOGS, FOLIC-ACID, TRIMETHOPRIM, METABOLISM, MECHANISM, SELECTION
journal title
PLANT BIOLOGY
Plant Biol.
volume
15
issue
1
pages
37 - 44
Web of Science type
Article
Web of Science id
000311848100005
JCR category
PLANT SCIENCES
JCR impact factor
2.405 (2013)
JCR rank
57/199 (2013)
JCR quartile
2 (2013)
ISSN
1435-8603
DOI
10.1111/j.1438-8677.2012.00602.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3140373
handle
http://hdl.handle.net/1854/LU-3140373
date created
2013-02-22 14:55:32
date last changed
2016-12-19 15:43:35
@article{3140373,
  abstract     = {Antifolates can impair the synthesis and/or function of folates in living organisms. Mechanisms of resistance or tolerance to antifolates have been mainly described in plants using the drug methotrexate. In this work, the antifolate trimethoprim (TMP) was used with the aim of revealing a novel mechanism of resistance. EMS mutagenised seeds from Arabidopsis were screened to isolate individuals insensitive to TMP. Genetic analysis revealed a homozygous recessive mutation that segregates with the phenotype of tolerance to 50 mu m TMP. Mapping analysis localised the mutation at the end of the short arm of chromosome 3. Preliminary characterisation demonstrated up-regulation of several genes from the folate biosynthetic pathway in the TMP insensitive mutant, and a slight increase in total folate content in the mutant as compared with the Col-0 control. Moreover, sequence analysis of the DHFR (dihydrofolate reductase) genes, which encode a known target for resistance to antifolates, did not reveal any changes. This study is the first report of a stable mutant insensitive (afi1) to the antifolate trimethoprim in plants, and suggests the existence of a novel mechanism of resistance to antifolates.},
  author       = {Navarrete, Oscar and Van Daele, Jeroen and Stove, Christophe and Lambert, Willy and Storojenko, Serguei and Van Der Straeten, Dominique},
  issn         = {1435-8603},
  journal      = {PLANT BIOLOGY},
  keyword      = {mapping,trimethoprim,folate biosynthesis,EMS mutant,Arabidopsis thaliana,Antifolate,RESISTANT CELL-LINES,REDUCTASE THYMIDYLATE SYNTHASE,DIHYDROFOLATE-REDUCTASE,METHOTREXATE-RESISTANCE,FOLATE ANALOGS,FOLIC-ACID,TRIMETHOPRIM,METABOLISM,MECHANISM,SELECTION},
  language     = {eng},
  number       = {1},
  pages        = {37--44},
  title        = {Isolation and characterisation of an antifolate insensitive (afi1) mutant of Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1111/j.1438-8677.2012.00602.x},
  volume       = {15},
  year         = {2013},
}

Chicago
Navarrete, Oscar, Jeroen Van Daele, Christophe Stove, Willy Lambert, Serguei Storojenko, and Dominique Van Der Straeten. 2013. “Isolation and Characterisation of an Antifolate Insensitive (afi1) Mutant of Arabidopsis Thaliana.” Plant Biology 15 (1): 37–44.
APA
Navarrete, O., Van Daele, J., Stove, C., Lambert, W., Storojenko, S., & Van Der Straeten, D. (2013). Isolation and characterisation of an antifolate insensitive (afi1) mutant of Arabidopsis thaliana. PLANT BIOLOGY, 15(1), 37–44.
Vancouver
1.
Navarrete O, Van Daele J, Stove C, Lambert W, Storojenko S, Van Der Straeten D. Isolation and characterisation of an antifolate insensitive (afi1) mutant of Arabidopsis thaliana. PLANT BIOLOGY. 2013;15(1):37–44.
MLA
Navarrete, Oscar, Jeroen Van Daele, Christophe Stove, et al. “Isolation and Characterisation of an Antifolate Insensitive (afi1) Mutant of Arabidopsis Thaliana.” PLANT BIOLOGY 15.1 (2013): 37–44. Print.