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CD64 distinguishes macrophages from dendritic cells in the gut and reveals the Th1-inducing role of mesenteric lymph node macrophages during colitis

Samira Tamoutounour, Sandrine Henri, Hugues Lelouard, Béatrice de Bovis, Colin de Haar, C Janneke van der Woude, Andrea M Woltman, Yasmin Reyal, Dominique Bonnet, Dorine Sichien UGent, et al. (2012) EUROPEAN JOURNAL OF IMMUNOLOGY. 42(12). p.3150-3166
abstract
Dendritic cells (DCs) and monocyte-derived macrophages (MFs) are key components of intestinal immunity. However, the lack of surface markers differentiating MFs from DCs has hampered understanding of their respective functions. Here, we demonstrate that, using CD64 expression, MFs can be distinguished from DCs in the intestine of both mice and humans. On that basis, we revisit the phenotype of intestinal DCs in the absence of contaminating MFs and we delineate a developmental pathway in the healthy intestine that leads from newly extravasated Ly-6Chi monocytes to intestinal MFs. We determine how inflammation impacts this pathway and show that T cell-mediated colitis is associated with massive recruitment of monocytes to the intestine and the mesenteric lymph node (MLN). There, these monocytes differentiate into inflammatory MFs endowed with phagocytic activity and the ability to produce inducible nitric oxide synthase. In the MLNs, inflammatory MFs are located in the T-cell zone and trigger the induction of proinflammatory T cells. Finally, T cell-mediated colitis develops irrespective of intestinal DC migration, an unexpected finding supporting an important role for MLN-resident inflammatory MFs in the etiology of T cell-mediated colitis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MFs, REGULATORY T-CELLS, Intestine, Dendritic cells, Colitis, CD64, MONOCYTES, RESPONSES, SUBSETS, RECEPTOR, MICE, INFLAMMATION, LAMINA PROPRIA, RETINOIC-ACID, BONE-MARROW
journal title
EUROPEAN JOURNAL OF IMMUNOLOGY
Eur. J. Immunol.
volume
42
issue
12
pages
3150 - 3166
Web of Science type
Article
Web of Science id
000312547800012
JCR category
IMMUNOLOGY
JCR impact factor
4.97 (2012)
JCR rank
26/134 (2012)
JCR quartile
1 (2012)
ISSN
0014-2980
DOI
10.1002/eji.201242847
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3139405
handle
http://hdl.handle.net/1854/LU-3139405
date created
2013-02-21 16:28:40
date last changed
2016-12-19 15:43:46
@article{3139405,
  abstract     = {Dendritic cells (DCs) and monocyte-derived macrophages (MFs) are key components of intestinal immunity. However, the lack of surface markers differentiating MFs from DCs has hampered understanding of their respective functions. Here, we demonstrate that, using CD64 expression, MFs can be distinguished from DCs in the intestine of both mice and humans. On that basis, we revisit the phenotype of intestinal DCs in the absence of contaminating MFs and we delineate a developmental pathway in the healthy intestine that leads from newly extravasated Ly-6Chi monocytes to intestinal MFs. We determine how inflammation impacts this pathway and show that T cell-mediated colitis is associated with massive recruitment of monocytes to the intestine and the mesenteric lymph node (MLN). There, these monocytes differentiate into inflammatory MFs endowed with phagocytic activity and the ability to produce inducible nitric oxide synthase. In the MLNs, inflammatory MFs are located in the T-cell zone and trigger the induction of proinflammatory T cells. Finally, T cell-mediated colitis develops irrespective of intestinal DC migration, an unexpected finding supporting an important role for MLN-resident inflammatory MFs in the etiology of T cell-mediated colitis.},
  author       = {Tamoutounour, Samira and Henri, Sandrine and Lelouard, Hugues and de Bovis, B{\'e}atrice and de Haar, Colin and van der Woude, C Janneke and Woltman, Andrea M and Reyal, Yasmin and Bonnet, Dominique and Sichien, Dorine and Bain, Calum C and Mowat, Allan McI and Reis e Sousa, Caetano and Poulin, Lionel F and Malissen, Bernard and Guilliams, Martin},
  issn         = {0014-2980},
  journal      = {EUROPEAN JOURNAL OF IMMUNOLOGY},
  keyword      = {MFs,REGULATORY T-CELLS,Intestine,Dendritic cells,Colitis,CD64,MONOCYTES,RESPONSES,SUBSETS,RECEPTOR,MICE,INFLAMMATION,LAMINA PROPRIA,RETINOIC-ACID,BONE-MARROW},
  language     = {eng},
  number       = {12},
  pages        = {3150--3166},
  title        = {CD64 distinguishes macrophages from dendritic cells in the gut and reveals the Th1-inducing role of mesenteric lymph node macrophages during colitis},
  url          = {http://dx.doi.org/10.1002/eji.201242847},
  volume       = {42},
  year         = {2012},
}

Chicago
Tamoutounour, Samira, Sandrine Henri, Hugues Lelouard, Béatrice de Bovis, Colin de Haar, C Janneke van der Woude, Andrea M Woltman, et al. 2012. “CD64 Distinguishes Macrophages from Dendritic Cells in the Gut and Reveals the Th1-inducing Role of Mesenteric Lymph Node Macrophages During Colitis.” European Journal of Immunology 42 (12): 3150–3166.
APA
Tamoutounour, S., Henri, S., Lelouard, H., de Bovis, B., de Haar, C., van der Woude, C. J., Woltman, A. M., et al. (2012). CD64 distinguishes macrophages from dendritic cells in the gut and reveals the Th1-inducing role of mesenteric lymph node macrophages during colitis. EUROPEAN JOURNAL OF IMMUNOLOGY, 42(12), 3150–3166.
Vancouver
1.
Tamoutounour S, Henri S, Lelouard H, de Bovis B, de Haar C, van der Woude CJ, et al. CD64 distinguishes macrophages from dendritic cells in the gut and reveals the Th1-inducing role of mesenteric lymph node macrophages during colitis. EUROPEAN JOURNAL OF IMMUNOLOGY. 2012;42(12):3150–66.
MLA
Tamoutounour, Samira, Sandrine Henri, Hugues Lelouard, et al. “CD64 Distinguishes Macrophages from Dendritic Cells in the Gut and Reveals the Th1-inducing Role of Mesenteric Lymph Node Macrophages During Colitis.” EUROPEAN JOURNAL OF IMMUNOLOGY 42.12 (2012): 3150–3166. Print.