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CD64 distinguishes macrophages from dendritic cells in the gut and reveals the Th1-inducing role of mesenteric lymph node macrophages during colitis

(2012) EUROPEAN JOURNAL OF IMMUNOLOGY. 42(12). p.3150-3166
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Abstract
Dendritic cells (DCs) and monocyte-derived macrophages (MFs) are key components of intestinal immunity. However, the lack of surface markers differentiating MFs from DCs has hampered understanding of their respective functions. Here, we demonstrate that, using CD64 expression, MFs can be distinguished from DCs in the intestine of both mice and humans. On that basis, we revisit the phenotype of intestinal DCs in the absence of contaminating MFs and we delineate a developmental pathway in the healthy intestine that leads from newly extravasated Ly-6Chi monocytes to intestinal MFs. We determine how inflammation impacts this pathway and show that T cell-mediated colitis is associated with massive recruitment of monocytes to the intestine and the mesenteric lymph node (MLN). There, these monocytes differentiate into inflammatory MFs endowed with phagocytic activity and the ability to produce inducible nitric oxide synthase. In the MLNs, inflammatory MFs are located in the T-cell zone and trigger the induction of proinflammatory T cells. Finally, T cell-mediated colitis develops irrespective of intestinal DC migration, an unexpected finding supporting an important role for MLN-resident inflammatory MFs in the etiology of T cell-mediated colitis.
Keywords
MFs, REGULATORY T-CELLS, Intestine, Dendritic cells, Colitis, CD64, MONOCYTES, RESPONSES, SUBSETS, RECEPTOR, MICE, INFLAMMATION, LAMINA PROPRIA, RETINOIC-ACID, BONE-MARROW

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Chicago
Tamoutounour, Samira, Sandrine Henri, Hugues Lelouard, Béatrice de Bovis, Colin de Haar, C Janneke van der Woude, Andrea M Woltman, et al. 2012. “CD64 Distinguishes Macrophages from Dendritic Cells in the Gut and Reveals the Th1-inducing Role of Mesenteric Lymph Node Macrophages During Colitis.” European Journal of Immunology 42 (12): 3150–3166.
APA
Tamoutounour, S., Henri, S., Lelouard, H., de Bovis, B., de Haar, C., van der Woude, C. J., Woltman, A. M., et al. (2012). CD64 distinguishes macrophages from dendritic cells in the gut and reveals the Th1-inducing role of mesenteric lymph node macrophages during colitis. EUROPEAN JOURNAL OF IMMUNOLOGY, 42(12), 3150–3166.
Vancouver
1.
Tamoutounour S, Henri S, Lelouard H, de Bovis B, de Haar C, van der Woude CJ, et al. CD64 distinguishes macrophages from dendritic cells in the gut and reveals the Th1-inducing role of mesenteric lymph node macrophages during colitis. EUROPEAN JOURNAL OF IMMUNOLOGY. 2012;42(12):3150–66.
MLA
Tamoutounour, Samira, Sandrine Henri, Hugues Lelouard, et al. “CD64 Distinguishes Macrophages from Dendritic Cells in the Gut and Reveals the Th1-inducing Role of Mesenteric Lymph Node Macrophages During Colitis.” EUROPEAN JOURNAL OF IMMUNOLOGY 42.12 (2012): 3150–3166. Print.
@article{3139405,
  abstract     = {Dendritic cells (DCs) and monocyte-derived macrophages (MFs) are key components of intestinal immunity. However, the lack of surface markers differentiating MFs from DCs has hampered understanding of their respective functions. Here, we demonstrate that, using CD64 expression, MFs can be distinguished from DCs in the intestine of both mice and humans. On that basis, we revisit the phenotype of intestinal DCs in the absence of contaminating MFs and we delineate a developmental pathway in the healthy intestine that leads from newly extravasated Ly-6Chi monocytes to intestinal MFs. We determine how inflammation impacts this pathway and show that T cell-mediated colitis is associated with massive recruitment of monocytes to the intestine and the mesenteric lymph node (MLN). There, these monocytes differentiate into inflammatory MFs endowed with phagocytic activity and the ability to produce inducible nitric oxide synthase. In the MLNs, inflammatory MFs are located in the T-cell zone and trigger the induction of proinflammatory T cells. Finally, T cell-mediated colitis develops irrespective of intestinal DC migration, an unexpected finding supporting an important role for MLN-resident inflammatory MFs in the etiology of T cell-mediated colitis.},
  author       = {Tamoutounour, Samira and Henri, Sandrine and Lelouard, Hugues and de Bovis, B{\'e}atrice and de Haar, Colin and van der Woude, C Janneke and Woltman, Andrea M and Reyal, Yasmin and Bonnet, Dominique and Sichien, Dorine and Bain, Calum C and Mowat, Allan McI and Reis e Sousa, Caetano and Poulin, Lionel F and Malissen, Bernard and Guilliams, Martin},
  issn         = {0014-2980},
  journal      = {EUROPEAN JOURNAL OF IMMUNOLOGY},
  keyword      = {MFs,REGULATORY T-CELLS,Intestine,Dendritic cells,Colitis,CD64,MONOCYTES,RESPONSES,SUBSETS,RECEPTOR,MICE,INFLAMMATION,LAMINA PROPRIA,RETINOIC-ACID,BONE-MARROW},
  language     = {eng},
  number       = {12},
  pages        = {3150--3166},
  title        = {CD64 distinguishes macrophages from dendritic cells in the gut and reveals the Th1-inducing role of mesenteric lymph node macrophages during colitis},
  url          = {http://dx.doi.org/10.1002/eji.201242847},
  volume       = {42},
  year         = {2012},
}

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