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Molecular characterization of Salmonella Enteritidis : comparison of an optimized multi-locus variable-number of tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis

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Abstract
Salmonella Enteritidis (SE) is a genetically homogenous serovar, which makes optimal subtype discrimination crucial for epidemiological research. This study describes the development and evaluation of an optimized multiple-locus variable number tandem-repeat assay (MLVA) for characterization of SE. The typeability and discriminatory power of this MLVA was determined on a selected collection of 60 SE isolates and compared with pulsed-field gel electrophoresis (PFGE) using restriction enzymes XbaI, NotI, or SfiI. In addition, the estimated Wallace coefficient (W) was calculated to assess the congruence of the typing methods. Selection of epidemiologically unrelated isolates and more related isolates (originating from layer farms) was also based on the given phage type (PT). When targeting six loci, MLVA generated 16 profiles, while PFGE produced 10, 9, and 16 pulsotypes using XbaI, NotI, and SfiI, respectively, for the entire strain collection. For the epidemiologically unrelated isolates, MLVA had the highest discriminatory power and showed good discrimination between isolates from different layer farms and among isolates from the same layer farm. MLVA performed together with PT showed higher discriminatory power compared to PFGE using one restriction enzyme together with PT. Results showed that combining PT with the optimized MLVA presented here provides a rapid typing tool with good discriminatory power for characterizing SE isolates of various origins and isolates originating from the same layer farm.
Keywords
SCHEME, PLASMID, DIVERSITY, TYPHIMURIUM, WALLACE COEFFICIENT, EUROPEAN COUNTRIES, SEROVAR ENTERITIDIS, ENTERICA SEROTYPE ENTERITIDIS, PHAGE TYPES 1, MICROBIAL TYPING METHODS

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Chicago
Dewaele, Isabelle, Geertrui Rasschaert, Sophie Bertrand, Christa Wildemauwe, Pierre Wattiau, Hein Imberechts, Lieve Herman, Richard Ducatelle, Koen De Reu, and Marc Heyndrickx. 2012. “Molecular Characterization of Salmonella Enteritidis : Comparison of an Optimized Multi-locus Variable-number of Tandem Repeat Analysis (MLVA) and Pulsed-field Gel Electrophoresis.” Foodborne Pathogens and Disease 9 (10): 885–895.
APA
Dewaele, Isabelle, Rasschaert, G., Bertrand, S., Wildemauwe, C., Wattiau, P., Imberechts, H., Herman, L., et al. (2012). Molecular characterization of Salmonella Enteritidis : comparison of an optimized multi-locus variable-number of tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis. FOODBORNE PATHOGENS AND DISEASE, 9(10), 885–895.
Vancouver
1.
Dewaele I, Rasschaert G, Bertrand S, Wildemauwe C, Wattiau P, Imberechts H, et al. Molecular characterization of Salmonella Enteritidis : comparison of an optimized multi-locus variable-number of tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis. FOODBORNE PATHOGENS AND DISEASE. 2012;9(10):885–95.
MLA
Dewaele, Isabelle, Geertrui Rasschaert, Sophie Bertrand, et al. “Molecular Characterization of Salmonella Enteritidis : Comparison of an Optimized Multi-locus Variable-number of Tandem Repeat Analysis (MLVA) and Pulsed-field Gel Electrophoresis.” FOODBORNE PATHOGENS AND DISEASE 9.10 (2012): 885–895. Print.
@article{3133760,
  abstract     = {Salmonella Enteritidis (SE) is a genetically homogenous serovar, which makes optimal subtype discrimination crucial for epidemiological research. This study describes the development and evaluation of an optimized multiple-locus variable number tandem-repeat assay (MLVA) for characterization of SE. The typeability and discriminatory power of this MLVA was determined on a selected collection of 60 SE isolates and compared with pulsed-field gel electrophoresis (PFGE) using restriction enzymes XbaI, NotI, or SfiI. In addition, the estimated Wallace coefficient (W) was calculated to assess the congruence of the typing methods. Selection of epidemiologically unrelated isolates and more related isolates (originating from layer farms) was also based on the given phage type (PT). When targeting six loci, MLVA generated 16 profiles, while PFGE produced 10, 9, and 16 pulsotypes using XbaI, NotI, and SfiI, respectively, for the entire strain collection. For the epidemiologically unrelated isolates, MLVA had the highest discriminatory power and showed good discrimination between isolates from different layer farms and among isolates from the same layer farm. MLVA performed together with PT showed higher discriminatory power compared to PFGE using one restriction enzyme together with PT. Results showed that combining PT with the optimized MLVA presented here provides a rapid typing tool with good discriminatory power for characterizing SE isolates of various origins and isolates originating from the same layer farm.},
  author       = {Dewaele, Isabelle and Rasschaert, Geertrui and Bertrand, Sophie and Wildemauwe, Christa and Wattiau, Pierre and Imberechts, Hein and Herman, Lieve and Ducatelle, Richard and De Reu, Koen and Heyndrickx, Marc},
  issn         = {1535-3141},
  journal      = {FOODBORNE PATHOGENS AND DISEASE},
  keyword      = {SCHEME,PLASMID,DIVERSITY,TYPHIMURIUM,WALLACE COEFFICIENT,EUROPEAN COUNTRIES,SEROVAR ENTERITIDIS,ENTERICA SEROTYPE ENTERITIDIS,PHAGE TYPES 1,MICROBIAL TYPING METHODS},
  language     = {eng},
  number       = {10},
  pages        = {885--895},
  title        = {Molecular characterization of Salmonella Enteritidis : comparison of an optimized multi-locus variable-number of tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis},
  url          = {http://dx.doi.org/10.1089/fpd.2012.1199},
  volume       = {9},
  year         = {2012},
}

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