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Brassinosteroid production and signaling differentially control cell division and expansion in the leaf

(2013) NEW PHYTOLOGIST. 197(2). p.490-502
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Brassinosteroid (BR) hormones control plant growth through acting on both cell expansion and division. Here, we examined the role of BRs in leaf growth using the Arabidopsis BR-deficient mutant constitutive photomorphogenesis and dwarfism (cpd). We show that the reduced size of cpd leaf blades is a result of a decrease in cell size and number, as well as in venation length and complexity. Kinematic growth analysis and tissue-specific marker gene expression revealed that the leaf phenotype of cpd is associated with a prolonged cell division phase and delayed differentiation. cpd-leaf-rescue experiments and leaf growth analysis of BR biosynthesis and signaling gain-of-function mutants showed that BR production and BR receptor-dependent signaling differentially control the balance between cell division and expansion in the leaf. Investigation of cell cycle markers in leaves of cpd revealed the accumulation of mitotic proteins independent of transcription. This correlated with an increase in cyclin-dependent kinase activity, suggesting a role for BRs in control of mitosis.
Keywords
PLASMA-MEMBRANE, AUXIN TRANSPORT, SHOOT GROWTH, SIZE CONTROL, PLANT DEVELOPMENT, DEVELOPING LEAVES, Arabidopsis, brassinosteroids, cell division, cpd, leaf, ARABIDOPSIS-THALIANA, GENE-EXPRESSION, INSENSITIVE1 RECEPTOR, MUTANT

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Citation

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MLA
Zhiponova, Miroslava, Isabelle Vanhoutte, Véronique Boudolf, et al. “Brassinosteroid Production and Signaling Differentially Control Cell Division and Expansion in the Leaf.” NEW PHYTOLOGIST 197.2 (2013): 490–502. Print.
APA
Zhiponova, M., Vanhoutte, I., Boudolf, V., Betti, C., Dhondt, S., Coppens, F., Mylle, E., et al. (2013). Brassinosteroid production and signaling differentially control cell division and expansion in the leaf. NEW PHYTOLOGIST, 197(2), 490–502.
Chicago author-date
Zhiponova, Miroslava, Isabelle Vanhoutte, Véronique Boudolf, Camilla Betti, Stijn Dhondt, Frederik Coppens, Evelien Mylle, et al. 2013. “Brassinosteroid Production and Signaling Differentially Control Cell Division and Expansion in the Leaf.” New Phytologist 197 (2): 490–502.
Chicago author-date (all authors)
Zhiponova, Miroslava, Isabelle Vanhoutte, Véronique Boudolf, Camilla Betti, Stijn Dhondt, Frederik Coppens, Evelien Mylle, Sara Maes, Mary-Paz González-García, Ana I Caño-Delgado, Dirk Inzé, Gerrit TS Beemster, Lieven De Veylder, and Eugenia Russinova. 2013. “Brassinosteroid Production and Signaling Differentially Control Cell Division and Expansion in the Leaf.” New Phytologist 197 (2): 490–502.
Vancouver
1.
Zhiponova M, Vanhoutte I, Boudolf V, Betti C, Dhondt S, Coppens F, et al. Brassinosteroid production and signaling differentially control cell division and expansion in the leaf. NEW PHYTOLOGIST. 2013;197(2):490–502.
IEEE
[1]
M. Zhiponova et al., “Brassinosteroid production and signaling differentially control cell division and expansion in the leaf,” NEW PHYTOLOGIST, vol. 197, no. 2, pp. 490–502, 2013.
@article{3131768,
  abstract     = {Brassinosteroid (BR) hormones control plant growth through acting on both cell expansion and division. Here, we examined the role of BRs in leaf growth using the Arabidopsis BR-deficient mutant constitutive photomorphogenesis and dwarfism (cpd).
We show that the reduced size of cpd leaf blades is a result of a decrease in cell size and number, as well as in venation length and complexity. Kinematic growth analysis and tissue-specific marker gene expression revealed that the leaf phenotype of cpd is associated with a prolonged cell division phase and delayed differentiation.
cpd-leaf-rescue experiments and leaf growth analysis of BR biosynthesis and signaling gain-of-function mutants showed that BR production and BR receptor-dependent signaling differentially control the balance between cell division and expansion in the leaf.
Investigation of cell cycle markers in leaves of cpd revealed the accumulation of mitotic proteins independent of transcription. This correlated with an increase in cyclin-dependent kinase activity, suggesting a role for BRs in control of mitosis.},
  author       = {Zhiponova, Miroslava and Vanhoutte, Isabelle and Boudolf, Véronique and Betti, Camilla and Dhondt, Stijn and Coppens, Frederik and Mylle, Evelien and Maes, Sara and González-García, Mary-Paz and Caño-Delgado, Ana I and Inzé, Dirk and Beemster, Gerrit TS and De Veylder, Lieven and Russinova, Eugenia},
  issn         = {0028-646X},
  journal      = {NEW PHYTOLOGIST},
  keywords     = {PLASMA-MEMBRANE,AUXIN TRANSPORT,SHOOT GROWTH,SIZE CONTROL,PLANT DEVELOPMENT,DEVELOPING LEAVES,Arabidopsis,brassinosteroids,cell division,cpd,leaf,ARABIDOPSIS-THALIANA,GENE-EXPRESSION,INSENSITIVE1 RECEPTOR,MUTANT},
  language     = {eng},
  number       = {2},
  pages        = {490--502},
  title        = {Brassinosteroid production and signaling differentially control cell division and expansion in the leaf},
  url          = {http://dx.doi.org/10.1111/nph.12036},
  volume       = {197},
  year         = {2013},
}

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