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Production of antibody derivatives in the methylotrophic yeast Pichia pastoris

Steve Schoonooghe (UGent) , Jannick Leoen (UGent) and Jurgen Haustraete (UGent)
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Abstract
New antibody derivatives are continuously being generated to interact with a range of therapeutic targets. The cost-effective and efficient production of these and other antibody derivatives is crucial for their further success. Here, we describe the construction of the expression vectors needed for heterologous expression of a Fab fragment in the yeast Pichia pastoris. The experimental conditions for lab-scale expressions are discussed, and an overview of an efficient purification strategy is presented.

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Chicago
Schoonooghe, Steve, Jannick Leoen, and Jurgen Haustraete. 2012. “Production of Antibody Derivatives in the Methylotrophic Yeast Pichia Pastoris.” In Antibody Engineering, ed. Patrick Chames, 907:325–340. Clifton, NJ, USA: Humana Press.
APA
Schoonooghe, S., Leoen, J., & Haustraete, J. (2012). Production of antibody derivatives in the methylotrophic yeast Pichia pastoris. In P. Chames (Ed.), Antibody engineering (Vol. 907, pp. 325–340). Clifton, NJ, USA: Humana Press.
Vancouver
1.
Schoonooghe S, Leoen J, Haustraete J. Production of antibody derivatives in the methylotrophic yeast Pichia pastoris. In: Chames P, editor. Antibody engineering. Clifton, NJ, USA: Humana Press; 2012. p. 325–40.
MLA
Schoonooghe, Steve, Jannick Leoen, and Jurgen Haustraete. “Production of Antibody Derivatives in the Methylotrophic Yeast Pichia Pastoris.” Antibody Engineering. Ed. Patrick Chames. Vol. 907. Clifton, NJ, USA: Humana Press, 2012. 325–340. Print.
@incollection{3129907,
  abstract     = {New antibody derivatives are continuously being generated to interact with a range of therapeutic targets. The cost-effective and efficient production of these and other antibody derivatives is crucial for their further success. Here, we describe the construction of the expression vectors needed for heterologous expression of a Fab fragment in the yeast Pichia pastoris. The experimental conditions for lab-scale expressions are discussed, and an overview of an efficient purification strategy is presented.},
  author       = {Schoonooghe, Steve and Leoen, Jannick and Haustraete, Jurgen},
  booktitle    = {Antibody engineering},
  editor       = {Chames, Patrick},
  isbn         = {9781617799747},
  issn         = {1064-3745},
  language     = {eng},
  pages        = {325--340},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Production of antibody derivatives in the methylotrophic yeast Pichia pastoris},
  url          = {http://dx.doi.org/10.1007/978-1-61779-974-7\_19},
  volume       = {907},
  year         = {2012},
}

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