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Pichia surface display: a tool for screening single domain antibodies

Kristof De Schutter (UGent) and Nico Callewaert (UGent)
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Abstract
Yeast surface display is being employed as an efficient tool for the isolation and engineering of traditional antibody fragments, both scFv and Fab, as well as single domain antibodies. Here we describe the protocols for a yeast surface display system developed in the methylothrophic yeast Pichia pastoris , the most commonly used yeast species for protein production. In this system the immune or maturated library of single domain antibodies is fused to the C-terminal domain of the Saccharomyces cerevisiae alpha-agglutinin gene ( SAG1 ) and expressed on the surface of P. pastoris cells. Labeling with ligands enables rapid and quantitative analysis in conjunction with isolation of single domain antibodies with the desired characteristics.

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MLA
De Schutter, Kristof, and Nico Callewaert. “Pichia Surface Display: A Tool for Screening Single Domain Antibodies.” Single Domain Antibodies : Methods and Protocols, edited by Dirk Saerens and Serge Muyldermans, vol. 911, Humana Press, 2012, pp. 125–34, doi:10.1007/978-1-61779-968-6_8.
APA
De Schutter, K., & Callewaert, N. (2012). Pichia surface display: a tool for screening single domain antibodies. In D. Saerens & S. Muyldermans (Eds.), Single domain antibodies : methods and protocols (Vol. 911, pp. 125–134). https://doi.org/10.1007/978-1-61779-968-6_8
Chicago author-date
De Schutter, Kristof, and Nico Callewaert. 2012. “Pichia Surface Display: A Tool for Screening Single Domain Antibodies.” In Single Domain Antibodies : Methods and Protocols, edited by Dirk Saerens and Serge Muyldermans, 911:125–34. Clifton, NJ, USA: Humana Press. https://doi.org/10.1007/978-1-61779-968-6_8.
Chicago author-date (all authors)
De Schutter, Kristof, and Nico Callewaert. 2012. “Pichia Surface Display: A Tool for Screening Single Domain Antibodies.” In Single Domain Antibodies : Methods and Protocols, ed by. Dirk Saerens and Serge Muyldermans, 911:125–134. Clifton, NJ, USA: Humana Press. doi:10.1007/978-1-61779-968-6_8.
Vancouver
1.
De Schutter K, Callewaert N. Pichia surface display: a tool for screening single domain antibodies. In: Saerens D, Muyldermans S, editors. Single domain antibodies : methods and protocols. Clifton, NJ, USA: Humana Press; 2012. p. 125–34.
IEEE
[1]
K. De Schutter and N. Callewaert, “Pichia surface display: a tool for screening single domain antibodies,” in Single domain antibodies : methods and protocols, vol. 911, D. Saerens and S. Muyldermans, Eds. Clifton, NJ, USA: Humana Press, 2012, pp. 125–134.
@incollection{3129880,
  abstract     = {{Yeast surface display is being employed as an efficient tool for the isolation and engineering of traditional antibody fragments, both scFv and Fab, as well as single domain antibodies. Here we describe the protocols for a yeast surface display system developed in the methylothrophic yeast Pichia pastoris , the most commonly used yeast species for protein production. In this system the immune or maturated library of single domain antibodies is fused to the C-terminal domain of the Saccharomyces cerevisiae alpha-agglutinin gene ( SAG1 ) and expressed on the surface of P. pastoris cells. Labeling with ligands enables rapid and quantitative analysis in conjunction with isolation of single domain antibodies with the desired characteristics.}},
  author       = {{De Schutter, Kristof and Callewaert, Nico}},
  booktitle    = {{Single domain antibodies : methods and protocols}},
  editor       = {{Saerens, Dirk and Muyldermans, Serge}},
  isbn         = {{9781617799686}},
  issn         = {{1064-3745}},
  language     = {{eng}},
  pages        = {{125--134}},
  publisher    = {{Humana Press}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Pichia surface display: a tool for screening single domain antibodies}},
  url          = {{http://doi.org/10.1007/978-1-61779-968-6_8}},
  volume       = {{911}},
  year         = {{2012}},
}

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