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Clinical procedure for colon carcinoma tissue sampling directly affects the cancer marker-capacity of VEGF family members

Sarah Pringels, Nancy Van Damme (UGent) , Bram De Craene (UGent) , Piet Pattyn (UGent) , Wim Ceelen (UGent) , Marc Peeters and Johan Grooten (UGent)
(2012) BMC CANCER. 12.
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Abstract
Background: mRNA levels of members of the Vascular Endothelial Growth Factor family (VEGF-A, -B, -C, -D, Placental Growth Factor/PlGF) have been investigated as tissue-based markers of colon cancer. These studies, which used specimens obtained by surgical resection or colonoscopic biopsy, yielded contradictory results. We studied the effect of the sampling method on the marker accuracy of VEGF family members. Methods: Comparative RT-qPCR analysis was performed on healthy colon and colon carcinoma samples obtained by biopsy (n = 38) or resection (n = 39) to measure mRNA expression levels of individual VEGF family members. mRNA levels of genes encoding the eicosanoid enzymes cyclooxygenase 2 (COX2) and 5-lipoxygenase (5-LOX) and of genes encoding the hypoxia markers glucose transporter 1 (GLUT-1) and carbonic anhydrase IX (CAIX) were included as markers for cellular stress and hypoxia. Results: Expression levels of COX2, 5-LOX, GLUT-1 and CAIX revealed the occurrence in healthy colon resection samples of hypoxic cellular stress and a concurrent increment of basal expression levels of VEGF family members. This increment abolished differential expression of VEGF-B and VEGF-C in matched carcinoma resection samples and created a surgery-induced underexpression of VEGF-D. VEGF-A and PlGF showed strong overexpression in carcinoma samples regardless of the sampling method. Conclusions: Sampling-induced hypoxia in resection samples but not in biopsy samples affects the marker-reliability of VEGF family members. Therefore, biopsy samples provide a more accurate report on VEGF family mRNA levels. Furthermore, this limited expression analysis proposes VEGF-A and PlGF as reliable, sampling procedure insensitive mRNA-markers for molecular diagnosis of colon cancer.
Keywords
Colon cancer, Sampling procedure, VEGF family members, Biomarker, Hypoxic stress, ENDOTHELIAL GROWTH-FACTOR, LYMPH-NODE METASTASIS, COLORECTAL-CANCER, TUMOR-ANGIOGENESIS, CELL-PROLIFERATION, PROSTAGLANDIN E-2, HYPOXIA, EXPRESSION, PROGRESSION, CYCLOOXYGENASE-2

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Chicago
Pringels, Sarah, Nancy Van Damme, Bram De Craene, Piet Pattyn, Wim Ceelen, Marc Peeters, and Johan Grooten. 2012. “Clinical Procedure for Colon Carcinoma Tissue Sampling Directly Affects the Cancer Marker-capacity of VEGF Family Members.” Bmc Cancer 12.
APA
Pringels, S., Van Damme, N., De Craene, B., Pattyn, P., Ceelen, W., Peeters, M., & Grooten, J. (2012). Clinical procedure for colon carcinoma tissue sampling directly affects the cancer marker-capacity of VEGF family members. BMC CANCER, 12.
Vancouver
1.
Pringels S, Van Damme N, De Craene B, Pattyn P, Ceelen W, Peeters M, et al. Clinical procedure for colon carcinoma tissue sampling directly affects the cancer marker-capacity of VEGF family members. BMC CANCER. 2012;12.
MLA
Pringels, Sarah, Nancy Van Damme, Bram De Craene, et al. “Clinical Procedure for Colon Carcinoma Tissue Sampling Directly Affects the Cancer Marker-capacity of VEGF Family Members.” BMC CANCER 12 (2012): n. pag. Print.
@article{3127231,
  abstract     = {Background: mRNA levels of members of the Vascular Endothelial Growth Factor family (VEGF-A, -B, -C, -D, Placental Growth Factor/PlGF) have been investigated as tissue-based markers of colon cancer. These studies, which used specimens obtained by surgical resection or colonoscopic biopsy, yielded contradictory results. We studied the effect of the sampling method on the marker accuracy of VEGF family members.
Methods: Comparative RT-qPCR analysis was performed on healthy colon and colon carcinoma samples obtained by biopsy (n = 38) or resection (n = 39) to measure mRNA expression levels of individual VEGF family members. mRNA levels of genes encoding the eicosanoid enzymes cyclooxygenase 2 (COX2) and 5-lipoxygenase (5-LOX) and of genes encoding the hypoxia markers glucose transporter 1 (GLUT-1) and carbonic anhydrase IX (CAIX) were included as markers for cellular stress and hypoxia.
Results: Expression levels of COX2, 5-LOX, GLUT-1 and CAIX revealed the occurrence in healthy colon resection samples of hypoxic cellular stress and a concurrent increment of basal expression levels of VEGF family members. This increment abolished differential expression of VEGF-B and VEGF-C in matched carcinoma resection samples and created a surgery-induced underexpression of VEGF-D. VEGF-A and PlGF showed strong overexpression in carcinoma samples regardless of the sampling method.
Conclusions: Sampling-induced hypoxia in resection samples but not in biopsy samples affects the marker-reliability of VEGF family members. Therefore, biopsy samples provide a more accurate report on VEGF family mRNA levels. Furthermore, this limited expression analysis proposes VEGF-A and PlGF as reliable, sampling procedure insensitive mRNA-markers for molecular diagnosis of colon cancer.},
  articleno    = {515},
  author       = {Pringels, Sarah and Van Damme, Nancy and De Craene, Bram and Pattyn, Piet and Ceelen, Wim and Peeters, Marc and Grooten, Johan},
  issn         = {1471-2407},
  journal      = {BMC CANCER},
  keyword      = {Colon cancer,Sampling procedure,VEGF family members,Biomarker,Hypoxic stress,ENDOTHELIAL GROWTH-FACTOR,LYMPH-NODE METASTASIS,COLORECTAL-CANCER,TUMOR-ANGIOGENESIS,CELL-PROLIFERATION,PROSTAGLANDIN E-2,HYPOXIA,EXPRESSION,PROGRESSION,CYCLOOXYGENASE-2},
  language     = {eng},
  pages        = {9},
  title        = {Clinical procedure for colon carcinoma tissue sampling directly affects the cancer marker-capacity of VEGF family members},
  url          = {http://dx.doi.org/10.1186/1471-2407-12-515},
  volume       = {12},
  year         = {2012},
}

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