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Establishment of an isotope dilution LC-MS/MS method revealing kinetics and distribution of co-occurring mycotoxins in rats

(2012) ANALYTICAL METHODS. 4(11). p.3708-3717
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Abstract
An isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) method with a fast sample preparation using homemade clean-up cartridges was developed for simultaneous determination of co-occurring mycotoxins exemplified with aflatoxin B1 (AFB1) and T-2 toxin (T-2) in representative biomatrices of rat plasma, heart, liver, kidney, spleen, lung and brain in a total run time of 7 min. The established approach using stable internal standards of [C-13(17)]-AFB1 and [C-13(24)]-T-2 was extensively validated by determining the specificity, linearity (R-2 >= 0.9990), sensitivity (lower limit of quantitation at 0.05 ng mL(-1)), accuracy (70.9-107.7%), precision (RSD <= 14.2%) and stability (>= 70.8%). Based on this methodological advance, the subsequent kinetics and tissue distribution after oral administration of 0.5 mg kg(-1) b.w. of both AFB1 and T-2 in rats were thoroughly studied. As revealed, both AFB1 and T-2 were rapidly eliminated with the half-life time (t(1/2)) in plasma of 8.44 +/- 4.02 h and 8.12 +/- 4.05 h, respectively. Moreover, AFB1 accumulated in all organs where the highest concentration was observed in liver (1.34 mu g kg(-1)), followed by kidney (0.76 mu g kg(-1)). Notably, only low levels of T-2 were observed in spleen (0.70 mu g kg(-1)) and in liver (0.15 mu g kg(-1)). The achieved data as supporting evidence would substantially promote the practical application of the proposed LC-MS/MS method for in vivo toxicokinetics and toxicity studies of co-occurring mycotoxins imitating natural incidence in rat system.
Keywords
T-2 TOXIN, TRADITIONAL CHINESE MEDICINES, AFLATOXIN B-1, METABOLIC PATHWAYS, LAYING HENS, TRICHOTHECENES, OCHRATOXIN, CLEARANCE, TOXICITY, TANDEM MASS-SPECTROMETRY, LC -MS/MS, co-occurring mycotoxins, Rat, Isotope dilution

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Citation

Please use this url to cite or link to this publication:

Chicago
Han, Zheng, Zhiyong Zhao, Suquan Song, Gang Liu, Jianxin Shi, Jingbo Zhang, Yucai Liao, et al. 2012. “Establishment of an Isotope Dilution LC-MS/MS Method Revealing Kinetics and Distribution of Co-occurring Mycotoxins in Rats.” Analytical Methods 4 (11): 3708–3717.
APA
Han, Z., Zhao, Z., Song, S., Liu, G., Shi, J., Zhang, J., Liao, Y., et al. (2012). Establishment of an isotope dilution LC-MS/MS method revealing kinetics and distribution of co-occurring mycotoxins in rats. ANALYTICAL METHODS, 4(11), 3708–3717.
Vancouver
1.
Han Z, Zhao Z, Song S, Liu G, Shi J, Zhang J, et al. Establishment of an isotope dilution LC-MS/MS method revealing kinetics and distribution of co-occurring mycotoxins in rats. ANALYTICAL METHODS. 2012;4(11):3708–17.
MLA
Han, Zheng, Zhiyong Zhao, Suquan Song, et al. “Establishment of an Isotope Dilution LC-MS/MS Method Revealing Kinetics and Distribution of Co-occurring Mycotoxins in Rats.” ANALYTICAL METHODS 4.11 (2012): 3708–3717. Print.
@article{3125386,
  abstract     = {An isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) method with a fast sample preparation using homemade clean-up cartridges was developed for simultaneous determination of co-occurring mycotoxins exemplified with aflatoxin B1 (AFB1) and T-2 toxin (T-2) in representative biomatrices of rat plasma, heart, liver, kidney, spleen, lung and brain in a total run time of 7 min. The established approach using stable internal standards of [C-13(17)]-AFB1 and [C-13(24)]-T-2 was extensively validated by determining the specificity, linearity (R-2 {\textrangle}= 0.9990), sensitivity (lower limit of quantitation at 0.05 ng mL(-1)), accuracy (70.9-107.7\%), precision (RSD {\textlangle}= 14.2\%) and stability ({\textrangle}= 70.8\%). Based on this methodological advance, the subsequent kinetics and tissue distribution after oral administration of 0.5 mg kg(-1) b.w. of both AFB1 and T-2 in rats were thoroughly studied. As revealed, both AFB1 and T-2 were rapidly eliminated with the half-life time (t(1/2)) in plasma of 8.44 +/- 4.02 h and 8.12 +/- 4.05 h, respectively. Moreover, AFB1 accumulated in all organs where the highest concentration was observed in liver (1.34 mu g kg(-1)), followed by kidney (0.76 mu g kg(-1)). Notably, only low levels of T-2 were observed in spleen (0.70 mu g kg(-1)) and in liver (0.15 mu g kg(-1)). The achieved data as supporting evidence would substantially promote the practical application of the proposed LC-MS/MS method for in vivo toxicokinetics and toxicity studies of co-occurring mycotoxins imitating natural incidence in rat system.},
  author       = {Han, Zheng and Zhao, Zhiyong and Song, Suquan and Liu, Gang and Shi, Jianxin and Zhang, Jingbo and Liao, Yucai and Zhang, Dabing and Wu, Yongjiang and De Saeger, Sarah and Wu, Aibo},
  issn         = {1759-9660},
  journal      = {ANALYTICAL METHODS},
  keyword      = {T-2 TOXIN,TRADITIONAL CHINESE MEDICINES,AFLATOXIN B-1,METABOLIC PATHWAYS,LAYING HENS,TRICHOTHECENES,OCHRATOXIN,CLEARANCE,TOXICITY,TANDEM MASS-SPECTROMETRY,LC -MS/MS,co-occurring mycotoxins,Rat,Isotope dilution},
  language     = {eng},
  number       = {11},
  pages        = {3708--3717},
  title        = {Establishment of an isotope dilution LC-MS/MS method revealing kinetics and distribution of co-occurring mycotoxins in rats},
  url          = {http://dx.doi.org/10.1039/c2ay25891a},
  volume       = {4},
  year         = {2012},
}

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