Advanced search
1 file | 1.15 MB

Aptamer-based molecular recognition of lysergamine, metergoline and small ergot alkaloids

Author
Organization
Abstract
Ergot alkaloids are mycotoxins produced by fungi of the genus Claviceps, which infect cereal crops and grasses. The uptake of ergot alkaloid contaminated cereal products can be lethal to humans and animals. For food safety assessment, analytical techniques are currently used to determine the presence of ergot alkaloids in food and feed samples. However, the number of samples which can be analyzed is limited, due to the cost of the equipment and the need for skilled personnel. In order to compensate for the lack of rapid tests for the detection of ergot alkaloids, the aim of this study was to develop a specific recognition element for ergot alkaloids, which could be further applied to produce a colorimetric reaction in the presence of these toxins. As recognition elements, single- stranded DNA ligands were selected by using an iterative selection procedure named SELEX, i.e., Systematic Evolution of Ligands by EXponential enrichment. After several selection cycles, the resulting aptamers were cloned and sequenced. A surface plasmon resonance analysis enabled determination of the dissociation constants of the complexes of aptamers and lysergamine. Dissociation constants in the nanomolar range were obtained with three selected aptamers. One of the selected aptamers, having a dissociation constant of 44 nM, was linked to gold nanoparticles and it was possible to produce a colorimetric reaction in the presence of lysergamine. This system could also be applied to small ergot alkaloids in an ergot contaminated flour sample.
Keywords
single-stranded nucleic acid, ergot alkaloids, surface plasmon resonance, SELEX procedure, HIGH-AFFINITY, DNA APTAMER, SELECTION, BINDING, LIGANDS, PATHOGENS, BEADS, FOOD

Downloads

  • RouahMartin 2012 IJMS 13 12 17138.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.15 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Rouah-Martin, Elsa, Jaytry Mehta, Bieke van Dorst, Sarah De Saeger, Peter Dubruel, Bert UW Maes, Filip Lemiere, et al. 2012. “Aptamer-based Molecular Recognition of Lysergamine, Metergoline and Small Ergot Alkaloids.” International Journal of Molecular Sciences 13 (12): 17138–17159.
APA
Rouah-Martin, E., Mehta, J., van Dorst, B., De Saeger, S., Dubruel, P., Maes, B. U., Lemiere, F., et al. (2012). Aptamer-based molecular recognition of lysergamine, metergoline and small ergot alkaloids. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 13(12), 17138–17159.
Vancouver
1.
Rouah-Martin E, Mehta J, van Dorst B, De Saeger S, Dubruel P, Maes BU, et al. Aptamer-based molecular recognition of lysergamine, metergoline and small ergot alkaloids. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2012;13(12):17138–59.
MLA
Rouah-Martin, Elsa, Jaytry Mehta, Bieke van Dorst, et al. “Aptamer-based Molecular Recognition of Lysergamine, Metergoline and Small Ergot Alkaloids.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 13.12 (2012): 17138–17159. Print.
@article{3125371,
  abstract     = {Ergot alkaloids are mycotoxins produced by fungi of the genus Claviceps, which infect cereal crops and grasses. The uptake of ergot alkaloid contaminated cereal products can be lethal to humans and animals. For food safety assessment, analytical techniques are currently used to determine the presence of ergot alkaloids in food and feed samples. However, the number of samples which can be analyzed is limited, due to the cost of the equipment and the need for skilled personnel. In order to compensate for the lack of rapid tests for the detection of ergot alkaloids, the aim of this study was to develop a specific recognition element for ergot alkaloids, which could be further applied to produce a colorimetric reaction in the presence of these toxins. As recognition elements, single- stranded DNA ligands were selected by using an iterative selection procedure named SELEX, i.e., Systematic Evolution of Ligands by EXponential enrichment. After several selection cycles, the resulting aptamers were cloned and sequenced. A surface plasmon resonance analysis enabled determination of the dissociation constants of the complexes of aptamers and lysergamine. Dissociation constants in the nanomolar range were obtained with three selected aptamers. One of the selected aptamers, having a dissociation constant of 44 nM, was linked to gold nanoparticles and it was possible to produce a colorimetric reaction in the presence of lysergamine. This system could also be applied to small ergot alkaloids in an ergot contaminated flour sample.},
  author       = {Rouah-Martin, Elsa and Mehta, Jaytry and van Dorst, Bieke and De Saeger, Sarah and Dubruel, Peter and Maes, Bert UW and Lemiere, Filip and Goormaghtigh, Erik and Daems, Devin and Herrebout, Wouter and van Hove, Fran\c{c}ois and Blust, Ronny and Robbens, Johan},
  issn         = {1422-0067},
  journal      = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
  keyword      = {single-stranded nucleic acid,ergot alkaloids,surface plasmon resonance,SELEX procedure,HIGH-AFFINITY,DNA APTAMER,SELECTION,BINDING,LIGANDS,PATHOGENS,BEADS,FOOD},
  language     = {eng},
  number       = {12},
  pages        = {17138--17159},
  title        = {Aptamer-based molecular recognition of lysergamine, metergoline and small ergot alkaloids},
  url          = {http://dx.doi.org/10.3390/ijms131217138},
  volume       = {13},
  year         = {2012},
}

Altmetric
View in Altmetric
Web of Science
Times cited: