Advanced search
1 file | 550.76 KB

A novel UPLC–MS–MS method for simultaneous determination of seven uremic retention toxins with cardiovascular relevance in chronic kidney disease patients

Jente Boelaert (UGent) , Frederic Lynen (UGent) , Griet Glorieux (UGent) , Sunny Eloot (UGent) , Maria Van Landschoot (UGent) , MARIA WATERLOOS (UGent) , Patrick Sandra (UGent) and Raymond Vanholder (UGent)
Author
Organization
Project
Development of Metabolic Analytical Platforms for the Characterization of Uremic Retention Compounds
Abstract
Chronic kidney disease (CKD) is a devastating illness characterized by accumulation of uremic retention solutes in the body. The objective of this study was to develop and validate a simple, rapid, and robust UPLC-MS-MS method for simultaneous determination, in serum, of seven organic acid uremic retention toxins, namely uric acid (UA), hippuric acid (HA), indoxylsulfate (IS), p-cresylglucuronide (pCG), p-cresylsulfate (pCS), indole-3-acetic acid (IAA), and 3-carboxy-4-methyl-5-propyl-2-furanpropionic acid (CMPF). Isotopically labeled internal standards (d(5)-HA; 1,3-N-15(2)-UA, and d(5)-IAA) were used to correct for variations in sample preparation and system performance. Separation on a C18 column was followed by negative electrospray ionization and tandem mass spectrometric detection. Accuracy was below the 15 % threshold. Within-day precision varied from 0.60 to 4.54 % and between-day precision was below 13.33 % for all compounds. The applicability of the method was evaluated by analyzing 78 serum samples originating both from healthy controls and from patients at different stages of CKD. These results were compared with those obtained by use of conventional HPLC-PDA-FLD methods. A good correlation was obtained between both methods for all compounds.
Keywords
uremic retention solutes, UPLC, chronic kidney disease, INDUCTION, EXPRESSION, REMOVAL, HEMODIALYSIS, SOLUTES, P-CRESYLSULPHATE, MASS-SPECTROMETRY, LIQUID-CHROMATOGRAPHY, URIC-ACID, INDOXYL SULFATE, Tandem mass spectrometry

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 550.76 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Boelaert, Jente, Frederic Lynen, Griet Glorieux, Sunny Eloot, Maria Van Landschoot, MARIA WATERLOOS, Patrick Sandra, and Raymond Vanholder. 2013. “A Novel UPLC–MS–MS Method for Simultaneous Determination of Seven Uremic Retention Toxins with Cardiovascular Relevance in Chronic Kidney Disease Patients.” Analytical and Bioanalytical Chemistry 405 (6): 1937–1947.
APA
Boelaert, Jente, Lynen, F., Glorieux, G., Eloot, S., Van Landschoot, M., WATERLOOS, M., Sandra, P., et al. (2013). A novel UPLC–MS–MS method for simultaneous determination of seven uremic retention toxins with cardiovascular relevance in chronic kidney disease patients. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 405(6), 1937–1947.
Vancouver
1.
Boelaert J, Lynen F, Glorieux G, Eloot S, Van Landschoot M, WATERLOOS M, et al. A novel UPLC–MS–MS method for simultaneous determination of seven uremic retention toxins with cardiovascular relevance in chronic kidney disease patients. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2013;405(6):1937–47.
MLA
Boelaert, Jente, Frederic Lynen, Griet Glorieux, et al. “A Novel UPLC–MS–MS Method for Simultaneous Determination of Seven Uremic Retention Toxins with Cardiovascular Relevance in Chronic Kidney Disease Patients.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 405.6 (2013): 1937–1947. Print.
@article{3118649,
  abstract     = {Chronic kidney disease (CKD) is a devastating illness characterized by accumulation of uremic retention solutes in the body. The objective of this study was to develop and validate a simple, rapid, and robust UPLC-MS-MS method for simultaneous determination, in serum, of seven organic acid uremic retention toxins, namely uric acid (UA), hippuric acid (HA), indoxylsulfate (IS), p-cresylglucuronide (pCG), p-cresylsulfate (pCS), indole-3-acetic acid (IAA), and 3-carboxy-4-methyl-5-propyl-2-furanpropionic acid (CMPF). Isotopically labeled internal standards (d(5)-HA; 1,3-N-15(2)-UA, and d(5)-IAA) were used to correct for variations in sample preparation and system performance. Separation on a C18 column was followed by negative electrospray ionization and tandem mass spectrometric detection. Accuracy was below the 15 \% threshold. Within-day precision varied from 0.60 to 4.54 \% and between-day precision was below 13.33 \% for all compounds. The applicability of the method was evaluated by analyzing 78 serum samples originating both from healthy controls and from patients at different stages of CKD. These results were compared with those obtained by use of conventional HPLC-PDA-FLD methods. A good correlation was obtained between both methods for all compounds.},
  author       = {Boelaert, Jente and Lynen, Frederic and Glorieux, Griet and Eloot, Sunny and Van Landschoot, Maria and WATERLOOS, MARIA and Sandra, Patrick and Vanholder, Raymond},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  language     = {eng},
  number       = {6},
  pages        = {1937--1947},
  title        = {A novel UPLC--MS--MS method for simultaneous determination of seven uremic retention toxins with cardiovascular relevance in chronic kidney disease patients},
  url          = {http://dx.doi.org/10.1007/s00216-012-6636-9},
  volume       = {405},
  year         = {2013},
}

Altmetric
View in Altmetric
Web of Science
Times cited: