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Brassinosteroids suppress rice defense against root-knot nematodes through antagonism with the jasmonate pathway

Kamrun Nahar (UGent) , Tina Kyndt (UGent) , Bettina Hause, Monica Höfte (UGent) and Godelieve Gheysen (UGent)
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Abstract
The importance of phytohormone balance is increasingly recognized as central to the outcome of plant pathogen interactions. Next to their well-known developmental role, brassinosteroids (BR) were recently found to be involved in plant innate immunity. In this study, we examined the role of BR in rice (Oryza sativa) innate immunity during infection with the root-knot nematode Meloidogyne graminicola, and we studied the inter-relationship with the jasmonate (JA) pathway. Exogenous epibrassinolide (BL) supply at low concentrations induced susceptibility in the roots whereas high concentrations of BL enforced systemic defense against this nematode. Upon high exogenous BL supply on the shoot, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) confirmed a strong feedback inhibitory effect, leading to reduced BR biosynthesis in the root. Moreover, we demonstrate that the immune suppressive effect of BR is at least partly due to negative cross-talk with the JA pathway. Mutants in the BR biosynthesis or signaling pathway accumulate slightly higher levels of the immediate JA-precursor 12-oxo-phytodienoic acid, and qRT-PCR data showed that the BR and JA pathway are mutually antagonistic in rice roots. Collectively, these results suggest that the balance between the BR and JA pathway is an effective regulator of the outcome of the rice M. graminicola interaction.
Keywords
GENE-EXPRESSION, PLANT IMMUNE-RESPONSES, HORMONE CROSSTALK, FUNGAL-INFECTION, SALICYLIC-ACID, ABSCISIC-ACID, BIOSYNTHESIS, ARABIDOPSIS, RESISTANCE, GROWTH

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MLA
Nahar, Kamrun, Tina Kyndt, Bettina Hause, et al. “Brassinosteroids Suppress Rice Defense Against Root-knot Nematodes Through Antagonism with the Jasmonate Pathway.” MOLECULAR PLANT-MICROBE INTERACTIONS 26.1 (2013): 106–115. Print.
APA
Nahar, K., Kyndt, T., Hause, B., Höfte, M., & Gheysen, G. (2013). Brassinosteroids suppress rice defense against root-knot nematodes through antagonism with the jasmonate pathway. MOLECULAR PLANT-MICROBE INTERACTIONS, 26(1), 106–115.
Chicago author-date
Nahar, Kamrun, Tina Kyndt, Bettina Hause, Monica Höfte, and Godelieve Gheysen. 2013. “Brassinosteroids Suppress Rice Defense Against Root-knot Nematodes Through Antagonism with the Jasmonate Pathway.” Molecular Plant-microbe Interactions 26 (1): 106–115.
Chicago author-date (all authors)
Nahar, Kamrun, Tina Kyndt, Bettina Hause, Monica Höfte, and Godelieve Gheysen. 2013. “Brassinosteroids Suppress Rice Defense Against Root-knot Nematodes Through Antagonism with the Jasmonate Pathway.” Molecular Plant-microbe Interactions 26 (1): 106–115.
Vancouver
1.
Nahar K, Kyndt T, Hause B, Höfte M, Gheysen G. Brassinosteroids suppress rice defense against root-knot nematodes through antagonism with the jasmonate pathway. MOLECULAR PLANT-MICROBE INTERACTIONS. 2013;26(1):106–15.
IEEE
[1]
K. Nahar, T. Kyndt, B. Hause, M. Höfte, and G. Gheysen, “Brassinosteroids suppress rice defense against root-knot nematodes through antagonism with the jasmonate pathway,” MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 26, no. 1, pp. 106–115, 2013.
@article{3117133,
  abstract     = {The importance of phytohormone balance is increasingly recognized as central to the outcome of plant pathogen interactions. Next to their well-known developmental role, brassinosteroids (BR) were recently found to be involved in plant innate immunity. In this study, we examined the role of BR in rice (Oryza sativa) innate immunity during infection with the root-knot nematode Meloidogyne graminicola, and we studied the inter-relationship with the jasmonate (JA) pathway. Exogenous epibrassinolide (BL) supply at low concentrations induced susceptibility in the roots whereas high concentrations of BL enforced systemic defense against this nematode. Upon high exogenous BL supply on the shoot, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) confirmed a strong feedback inhibitory effect, leading to reduced BR biosynthesis in the root. Moreover, we demonstrate that the immune suppressive effect of BR is at least partly due to negative cross-talk with the JA pathway. Mutants in the BR biosynthesis or signaling pathway accumulate slightly higher levels of the immediate JA-precursor 12-oxo-phytodienoic acid, and qRT-PCR data showed that the BR and JA pathway are mutually antagonistic in rice roots. Collectively, these results suggest that the balance between the BR and JA pathway is an effective regulator of the outcome of the rice M. graminicola interaction.},
  author       = {Nahar, Kamrun and Kyndt, Tina and Hause, Bettina and Höfte, Monica and Gheysen, Godelieve},
  issn         = {0894-0282},
  journal      = {MOLECULAR PLANT-MICROBE INTERACTIONS},
  keywords     = {GENE-EXPRESSION,PLANT IMMUNE-RESPONSES,HORMONE CROSSTALK,FUNGAL-INFECTION,SALICYLIC-ACID,ABSCISIC-ACID,BIOSYNTHESIS,ARABIDOPSIS,RESISTANCE,GROWTH},
  language     = {eng},
  number       = {1},
  pages        = {106--115},
  title        = {Brassinosteroids suppress rice defense against root-knot nematodes through antagonism with the jasmonate pathway},
  url          = {http://dx.doi.org/10.1094/MPMI-05-12-0108-FI},
  volume       = {26},
  year         = {2013},
}

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