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A green fluorescent protein-transformed Mycosphaerella fijiensis strain shows increased aggressiveness on banana

(2012) AUSTRALASIAN PLANT PATHOLOGY. 41(6). p.645-647
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Abstract
The fungal pathogen Mycosphaerella fijiensis, causal agent of black leaf streak disease of bananas and plantains, was transformed with a green fluorescent protein-carrying construct by using a restriction enzyme-mediated integration methodology. A quantitative polymerase chain reaction was adapted to estimate transgene copy number and pathogenicity assays with three banana genotypes with dissimilar reactions to M. fijiensis infection were performed to characterize the transformants. Transgene insertion varied from one to five copies per genome among four random selected transformants. All M. fijiensis strains produced typical symptoms of the black leaf streak disease on the three banana genotypes assayed. Interestingly, the GFP-18 transformant showed increased aggressiveness on susceptible 'Grande naine' and resistant 'Yangambi km5' plants demonstrating that mutation events in M. fijiensis can increase virulence.
Keywords
Genetic transformation, Black Sigatoka, Musa spp., Mycospherella fijiensis, Pathogenicity, LEAF STREAK DISEASE, INFECTION, TOOL

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Citation

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MLA
Portal Villafaña, Orelvis, Mayra Acosta-Suárez, Bárbara Ocaña, et al. “A Green Fluorescent Protein-transformed Mycosphaerella Fijiensis Strain Shows Increased Aggressiveness on Banana.” AUSTRALASIAN PLANT PATHOLOGY 41.6 (2012): 645–647. Print.
APA
Portal Villafaña, O., Acosta-Suárez, M., Ocaña, B., Schäfer, W., Jiménez, E., & Höfte, M. (2012). A green fluorescent protein-transformed Mycosphaerella fijiensis strain shows increased aggressiveness on banana. AUSTRALASIAN PLANT PATHOLOGY, 41(6), 645–647.
Chicago author-date
Portal Villafaña, Orelvis, Mayra Acosta-Suárez, Bárbara Ocaña, Wilhelm Schäfer, Elio Jiménez, and Monica Höfte. 2012. “A Green Fluorescent Protein-transformed Mycosphaerella Fijiensis Strain Shows Increased Aggressiveness on Banana.” Australasian Plant Pathology 41 (6): 645–647.
Chicago author-date (all authors)
Portal Villafaña, Orelvis, Mayra Acosta-Suárez, Bárbara Ocaña, Wilhelm Schäfer, Elio Jiménez, and Monica Höfte. 2012. “A Green Fluorescent Protein-transformed Mycosphaerella Fijiensis Strain Shows Increased Aggressiveness on Banana.” Australasian Plant Pathology 41 (6): 645–647.
Vancouver
1.
Portal Villafaña O, Acosta-Suárez M, Ocaña B, Schäfer W, Jiménez E, Höfte M. A green fluorescent protein-transformed Mycosphaerella fijiensis strain shows increased aggressiveness on banana. AUSTRALASIAN PLANT PATHOLOGY. 2012;41(6):645–7.
IEEE
[1]
O. Portal Villafaña, M. Acosta-Suárez, B. Ocaña, W. Schäfer, E. Jiménez, and M. Höfte, “A green fluorescent protein-transformed Mycosphaerella fijiensis strain shows increased aggressiveness on banana,” AUSTRALASIAN PLANT PATHOLOGY, vol. 41, no. 6, pp. 645–647, 2012.
@article{3117103,
  abstract     = {The fungal pathogen Mycosphaerella fijiensis, causal agent of black leaf streak disease of bananas and plantains, was transformed with a green fluorescent protein-carrying construct by using a restriction enzyme-mediated integration methodology. A quantitative polymerase chain reaction was adapted to estimate transgene copy number and pathogenicity assays with three banana genotypes with dissimilar reactions to M. fijiensis infection were performed to characterize the transformants. Transgene insertion varied from one to five copies per genome among four random selected transformants. All M. fijiensis strains produced typical symptoms of the black leaf streak disease on the three banana genotypes assayed. Interestingly, the GFP-18 transformant showed increased aggressiveness on susceptible 'Grande naine' and resistant 'Yangambi km5' plants demonstrating that mutation events in M. fijiensis can increase virulence.},
  author       = {Portal Villafaña, Orelvis and Acosta-Suárez, Mayra and Ocaña, Bárbara and Schäfer, Wilhelm and Jiménez, Elio and Höfte, Monica},
  issn         = {0815-3191},
  journal      = {AUSTRALASIAN PLANT PATHOLOGY},
  keywords     = {Genetic transformation,Black Sigatoka,Musa spp.,Mycospherella fijiensis,Pathogenicity,LEAF STREAK DISEASE,INFECTION,TOOL},
  language     = {eng},
  number       = {6},
  pages        = {645--647},
  title        = {A green fluorescent protein-transformed Mycosphaerella fijiensis strain shows increased aggressiveness on banana},
  url          = {http://dx.doi.org/10.1007/s13313-012-0155-1},
  volume       = {41},
  year         = {2012},
}

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