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Phylogeography and virulence structure of the powdery mildew population on its 'new' host triticale

Veronique Troch (UGent) , Kris Audenaert (UGent) , Boris Bekaert (UGent) , Monica Höfte (UGent) and Geert Haesaert (UGent)
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Abstract
Background: Powdery mildew, caused by the obligate biotrophic fungus Blumeria graminis, is a major problem in cereal production as it can reduce quality and yield. B. graminis has evolved eight distinct formae speciales (f.sp.) which display strict host specialization. In the last decade, powdery mildew has emerged on triticale, the artificial intergeneric hybrid between wheat and rye. This emergence is probably triggered by a host range expansion of the wheat powdery mildew B. graminis f.sp. tritici. To gain more precise information about the evolutionary processes that led to this host range expansion, we pursued a combined pathological and genetic approach. Results: B. graminis isolates were sampled from triticale, wheat and rye from different breeding regions in Europe. Pathogenicity tests showed that isolates collected from triticale are highly pathogenic on most of the tested triticale cultivars. Moreover, these isolates were also able to infect several wheat cultivars (their previous hosts), although a lower aggressiveness was observed compared to isolates collected from wheat. Phylogenetic analysis of nuclear gene regions identified two statistically significant clades, which to a certain extent correlated with pathogenicity. No differences in virulence profiles were found among the sampled regions, but the distribution of genetic variation demonstrated to be geography dependent. A multilocus haplotype network showed that haplotypes pathogenic on triticale are distributed at different sites in the network, but always clustered at or near the tips of the network. Conclusions: This study reveals a genetic structure in B. graminis with population differentiation according to geography and host specificity. In addition, evidence is brought forward demonstrating that the host range expansion of wheat isolates to the new host triticale occurred recently and multiple times at different locations in Europe.
Keywords
ERYSIPHE-GRAMINIS, MAGNAPORTHE-ORYZAE, BLUMERIA-GRAMINIS, F SP HORDEI, Nephron-sparing surgery, Oncologic outcomes, Robotic partial nephrectomy, Renal cell carcinoma, Partial nephrectomy, CLUSTAL-W, WHEAT, FUNGUS, EXPANSION, RESISTANCE, DOMESTICATION

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Chicago
Troch, Veronique, Kris Audenaert, Boris Bekaert, Monica Höfte, and Geert Haesaert. 2012. “Phylogeography and Virulence Structure of the Powdery Mildew Population on Its ‘New’ Host Triticale.” Bmc Evolutionary Biology 12.
APA
Troch, V., Audenaert, K., Bekaert, B., Höfte, M., & Haesaert, G. (2012). Phylogeography and virulence structure of the powdery mildew population on its “new” host triticale. BMC EVOLUTIONARY BIOLOGY, 12.
Vancouver
1.
Troch V, Audenaert K, Bekaert B, Höfte M, Haesaert G. Phylogeography and virulence structure of the powdery mildew population on its “new” host triticale. BMC EVOLUTIONARY BIOLOGY. 2012;12.
MLA
Troch, Veronique, Kris Audenaert, Boris Bekaert, et al. “Phylogeography and Virulence Structure of the Powdery Mildew Population on Its ‘New’ Host Triticale.” BMC EVOLUTIONARY BIOLOGY 12 (2012): n. pag. Print.
@article{3117096,
  abstract     = {Background: Powdery mildew, caused by the obligate biotrophic fungus Blumeria graminis, is a major problem in cereal production as it can reduce quality and yield. B. graminis has evolved eight distinct formae speciales (f.sp.) which display strict host specialization. In the last decade, powdery mildew has emerged on triticale, the artificial intergeneric hybrid between wheat and rye. This emergence is probably triggered by a host range expansion of the wheat powdery mildew B. graminis f.sp. tritici. To gain more precise information about the evolutionary processes that led to this host range expansion, we pursued a combined pathological and genetic approach. 
Results: B. graminis isolates were sampled from triticale, wheat and rye from different breeding regions in Europe. Pathogenicity tests showed that isolates collected from triticale are highly pathogenic on most of the tested triticale cultivars. Moreover, these isolates were also able to infect several wheat cultivars (their previous hosts), although a lower aggressiveness was observed compared to isolates collected from wheat. Phylogenetic analysis of nuclear gene regions identified two statistically significant clades, which to a certain extent correlated with pathogenicity. No differences in virulence profiles were found among the sampled regions, but the distribution of genetic variation demonstrated to be geography dependent. A multilocus haplotype network showed that haplotypes pathogenic on triticale are distributed at different sites in the network, but always clustered at or near the tips of the network. 
Conclusions: This study reveals a genetic structure in B. graminis with population differentiation according to geography and host specificity. In addition, evidence is brought forward demonstrating that the host range expansion of wheat isolates to the new host triticale occurred recently and multiple times at different locations in Europe.},
  articleno    = {76},
  author       = {Troch, Veronique and Audenaert, Kris and Bekaert, Boris and H{\"o}fte, Monica and Haesaert, Geert},
  issn         = {1471-2148},
  journal      = {BMC EVOLUTIONARY BIOLOGY},
  keyword      = {ERYSIPHE-GRAMINIS,MAGNAPORTHE-ORYZAE,BLUMERIA-GRAMINIS,F SP HORDEI,Nephron-sparing surgery,Oncologic outcomes,Robotic partial nephrectomy,Renal cell carcinoma,Partial nephrectomy,CLUSTAL-W,WHEAT,FUNGUS,EXPANSION,RESISTANCE,DOMESTICATION},
  language     = {eng},
  pages        = {12},
  title        = {Phylogeography and virulence structure of the powdery mildew population on its 'new' host triticale},
  url          = {http://dx.doi.org/10.1186/1471-2148-12-76},
  volume       = {12},
  year         = {2012},
}

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