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Granulysin produced by globule leukocytes as a potential key element in the development of vaccine-induced immunity against Ostertgia ostertagi

Frederik Van Meulder UGent, Manuela Rinaldi UGent, Stefanie Van Coppernolle UGent, Edwin Claerebout UGent, Jozef Vercruysse UGent and Marc Geldhof (2012) Joint NVP/BSP scientific meeting, Abstracts.
abstract
Ostertagia ostertagi is considered the most economically important bovine parasite. An experimental host-protective vaccine against Ostertagia ostertagi was developed based on ASP-proteins derived from excretory-secretory material of the helminth. Further optimization and commercialization of this vaccine, however, requires a thorough understanding of the vaccine-induced immune response. Previous studies in which immune cell counts and cytokine transcription levels were investigated, did not detect differences between vaccinated and susceptible animals. Therefore, a broader whole-transcriptomic approach using a micro-array was applied. Interesting targets discovered by the micro-array were further analysed on a protein level. This approach revealed a significant upregulation in expression of the granule-proteins granulysin (GNLY) and granzyme B (GZMB) and the high affinity IgE-receptor 1 (FCER1A) in infected vaccinated animals compared to infected non-vaccinated animals. Moreover, these genes significantly correlated with faecal egg count reduction and worm counts. We have further shown that granulysin is produced by globule leukocytes in the abomasum and is secreted into the mucus, presumably through antibody-dependent triggering of the IgE receptor 1. Correlations between globule leukocytes and protection, and the suggestion of a protective agent being present in the mucus, have been described previously. This is however the first time to our knowledge that such an agent is actually identified. Our results, along with the knowledge that granulysin has recently been associated with immunity against a variety of helminths, render granulysin a very interesting topic for further research.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
Joint NVP/BSP scientific meeting, Abstracts
publisher
Nederlandse Vereniging voor Parasitologie (NVP) ; Belgian Society for Parasitology (BSP)
conference name
Joint NVP/BSP scientific meeting 2012 : Challenges for the control of parasites
conference location
Antwerp, Belgium
conference start
2012-10-19
conference end
2012-10-19
language
English
UGent publication?
yes
classification
C3
id
3116818
handle
http://hdl.handle.net/1854/LU-3116818
date created
2013-01-31 10:50:34
date last changed
2016-12-19 15:36:29
@inproceedings{3116818,
  abstract     = {Ostertagia ostertagi is considered the most economically important bovine parasite. An experimental host-protective vaccine against Ostertagia ostertagi was developed based on ASP-proteins derived from excretory-secretory material of the helminth. Further optimization and commercialization of this vaccine, however, requires a thorough understanding of the vaccine-induced immune response.
Previous studies in which immune cell counts and cytokine transcription levels were investigated, did not detect differences between vaccinated and susceptible animals. Therefore, a broader whole-transcriptomic approach using a micro-array was applied. Interesting targets discovered by the micro-array were further analysed on a protein level.
This approach revealed a significant upregulation in expression of the granule-proteins granulysin (GNLY) and granzyme B (GZMB) and the high affinity IgE-receptor 1 (FCER1A) in infected vaccinated animals compared to infected non-vaccinated animals. Moreover, these genes significantly correlated with faecal egg count reduction and worm counts. We have further shown that granulysin is produced by globule leukocytes in the abomasum and is secreted into the mucus, presumably through antibody-dependent triggering of the IgE receptor 1.
Correlations between globule leukocytes and protection, and the suggestion of a protective agent being present in the mucus, have been described previously. This is however the first time to our knowledge that such an agent is actually identified.
Our results, along with the knowledge that granulysin has recently been associated with immunity against a variety of helminths, render granulysin a very interesting topic for further research.},
  author       = {Van Meulder, Frederik and Rinaldi, Manuela and Van Coppernolle, Stefanie and Claerebout, Edwin and Vercruysse, Jozef and Geldhof, Marc},
  booktitle    = {Joint NVP/BSP scientific meeting, Abstracts},
  language     = {eng},
  location     = {Antwerp, Belgium},
  publisher    = {Nederlandse Vereniging voor Parasitologie (NVP) ; Belgian Society for Parasitology (BSP)},
  title        = {Granulysin produced by globule leukocytes as a potential key element in the development of vaccine-induced immunity against Ostertgia ostertagi},
  year         = {2012},
}

Chicago
Van Meulder, Frederik, Manuela Rinaldi, Stefanie Van Coppernolle, Edwin Claerebout, Jozef Vercruysse, and Marc Geldhof. 2012. “Granulysin Produced by Globule Leukocytes as a Potential Key Element in the Development of Vaccine-induced Immunity Against Ostertgia Ostertagi.” In Joint NVP/BSP Scientific Meeting, Abstracts. Nederlandse Vereniging voor Parasitologie (NVP) ; Belgian Society for Parasitology (BSP).
APA
Van Meulder, Frederik, Rinaldi, M., Van Coppernolle, S., Claerebout, E., Vercruysse, J., & Geldhof, M. (2012). Granulysin produced by globule leukocytes as a potential key element in the development of vaccine-induced immunity against Ostertgia ostertagi. Joint NVP/BSP scientific meeting, Abstracts. Presented at the Joint NVP/BSP scientific meeting 2012 : Challenges for the control of parasites, Nederlandse Vereniging voor Parasitologie (NVP) ; Belgian Society for Parasitology (BSP).
Vancouver
1.
Van Meulder F, Rinaldi M, Van Coppernolle S, Claerebout E, Vercruysse J, Geldhof M. Granulysin produced by globule leukocytes as a potential key element in the development of vaccine-induced immunity against Ostertgia ostertagi. Joint NVP/BSP scientific meeting, Abstracts. Nederlandse Vereniging voor Parasitologie (NVP) ; Belgian Society for Parasitology (BSP); 2012.
MLA
Van Meulder, Frederik, Manuela Rinaldi, Stefanie Van Coppernolle, et al. “Granulysin Produced by Globule Leukocytes as a Potential Key Element in the Development of Vaccine-induced Immunity Against Ostertgia Ostertagi.” Joint NVP/BSP Scientific Meeting, Abstracts. Nederlandse Vereniging voor Parasitologie (NVP) ; Belgian Society for Parasitology (BSP), 2012. Print.