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Granulysin produced by globule leukocytes as a potential key element in the development of vaccine-induced immunity against Ostertgia ostertagi

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Abstract
Ostertagia ostertagi is considered the most economically important bovine parasite. An experimental host-protective vaccine against Ostertagia ostertagi was developed based on ASP-proteins derived from excretory-secretory material of the helminth. Further optimization and commercialization of this vaccine, however, requires a thorough understanding of the vaccine-induced immune response. Previous studies in which immune cell counts and cytokine transcription levels were investigated, did not detect differences between vaccinated and susceptible animals. Therefore, a broader whole-transcriptomic approach using a micro-array was applied. Interesting targets discovered by the micro-array were further analysed on a protein level. This approach revealed a significant upregulation in expression of the granule-proteins granulysin (GNLY) and granzyme B (GZMB) and the high affinity IgE-receptor 1 (FCER1A) in infected vaccinated animals compared to infected non-vaccinated animals. Moreover, these genes significantly correlated with faecal egg count reduction and worm counts. We have further shown that granulysin is produced by globule leukocytes in the abomasum and is secreted into the mucus, presumably through antibody-dependent triggering of the IgE receptor 1. Correlations between globule leukocytes and protection, and the suggestion of a protective agent being present in the mucus, have been described previously. This is however the first time to our knowledge that such an agent is actually identified. Our results, along with the knowledge that granulysin has recently been associated with immunity against a variety of helminths, render granulysin a very interesting topic for further research.

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Chicago
Van Meulder, Frederik, Manuela Rinaldi, Stefanie Van Coppernolle, Edwin Claerebout, Jozef Vercruysse, and Marc Geldhof. 2012. “Granulysin Produced by Globule Leukocytes as a Potential Key Element in the Development of Vaccine-induced Immunity Against Ostertgia Ostertagi.” In Joint NVP/BSP Scientific Meeting, Abstracts. Nederlandse Vereniging voor Parasitologie (NVP) ; Belgian Society for Parasitology (BSP).
APA
Van Meulder, Frederik, Rinaldi, M., Van Coppernolle, S., Claerebout, E., Vercruysse, J., & Geldhof, M. (2012). Granulysin produced by globule leukocytes as a potential key element in the development of vaccine-induced immunity against Ostertgia ostertagi. Joint NVP/BSP scientific meeting, Abstracts. Presented at the Joint NVP/BSP scientific meeting 2012 : Challenges for the control of parasites, Nederlandse Vereniging voor Parasitologie (NVP) ; Belgian Society for Parasitology (BSP).
Vancouver
1.
Van Meulder F, Rinaldi M, Van Coppernolle S, Claerebout E, Vercruysse J, Geldhof M. Granulysin produced by globule leukocytes as a potential key element in the development of vaccine-induced immunity against Ostertgia ostertagi. Joint NVP/BSP scientific meeting, Abstracts. Nederlandse Vereniging voor Parasitologie (NVP) ; Belgian Society for Parasitology (BSP); 2012.
MLA
Van Meulder, Frederik, Manuela Rinaldi, Stefanie Van Coppernolle, et al. “Granulysin Produced by Globule Leukocytes as a Potential Key Element in the Development of Vaccine-induced Immunity Against Ostertgia Ostertagi.” Joint NVP/BSP Scientific Meeting, Abstracts. Nederlandse Vereniging voor Parasitologie (NVP) ; Belgian Society for Parasitology (BSP), 2012. Print.
@inproceedings{3116818,
  abstract     = {Ostertagia ostertagi is considered the most economically important bovine parasite. An experimental host-protective vaccine against Ostertagia ostertagi was developed based on ASP-proteins derived from excretory-secretory material of the helminth. Further optimization and commercialization of this vaccine, however, requires a thorough understanding of the vaccine-induced immune response.
Previous studies in which immune cell counts and cytokine transcription levels were investigated, did not detect differences between vaccinated and susceptible animals. Therefore, a broader whole-transcriptomic approach using a micro-array was applied. Interesting targets discovered by the micro-array were further analysed on a protein level.
This approach revealed a significant upregulation in expression of the granule-proteins granulysin (GNLY) and granzyme B (GZMB) and the high affinity IgE-receptor 1 (FCER1A) in infected vaccinated animals compared to infected non-vaccinated animals. Moreover, these genes significantly correlated with faecal egg count reduction and worm counts. We have further shown that granulysin is produced by globule leukocytes in the abomasum and is secreted into the mucus, presumably through antibody-dependent triggering of the IgE receptor 1.
Correlations between globule leukocytes and protection, and the suggestion of a protective agent being present in the mucus, have been described previously. This is however the first time to our knowledge that such an agent is actually identified.
Our results, along with the knowledge that granulysin has recently been associated with immunity against a variety of helminths, render granulysin a very interesting topic for further research.},
  author       = {Van Meulder, Frederik and Rinaldi, Manuela and Van Coppernolle, Stefanie and Claerebout, Edwin and Vercruysse, Jozef and Geldhof, Marc},
  booktitle    = {Joint NVP/BSP scientific meeting, Abstracts},
  language     = {eng},
  location     = {Antwerp, Belgium},
  publisher    = {Nederlandse Vereniging voor Parasitologie (NVP) ; Belgian Society for Parasitology (BSP)},
  title        = {Granulysin produced by globule leukocytes as a potential key element in the development of vaccine-induced immunity against Ostertgia ostertagi},
  year         = {2012},
}